BBa_K299000
1
BBa_K299000
RBS measurement device J23100 J61100 GFP
2010-07-18T11:00:00Z
2015-05-08T01:11:49Z
registry
Anderson's RBS under J23100 synthetic promoter followed by GFP_double_terminator (I13401=E0040+B0015)
false
false
_419_
0
4959
9
It's complicated
true
J23100 used here is part of the Anderon RBS biobrick J61100.
(Anderson RBSes comes in modified vectors)
false
Anna Olchowik
component2077273
1
BBa_J23100
component2077280
1
BBa_B0010
component2077282
1
BBa_K299801
component2077279
1
BBa_K299801
component2077276
1
BBa_K299802
component2077283
1
BBa_B0012
component2077274
1
BBa_K299107
component2077275
1
BBa_J61100
component2077278
1
BBa_E0040
annotation2077274
1
BBa_K299107
range2077274
1
36
43
annotation2077282
1
BBa_K299801
range2077282
1
870
877
annotation2077275
1
BBa_J61100
range2077275
1
44
55
annotation2077276
1
BBa_K299802
range2077276
1
56
61
annotation2077280
1
BBa_B0010
range2077280
1
790
869
annotation2077279
1
BBa_K299801
range2077279
1
782
789
annotation2077278
1
BBa_E0040
range2077278
1
62
781
annotation2077283
1
BBa_B0012
range2077283
1
878
918
annotation2077273
1
BBa_J23100
range2077273
1
1
35
BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
{{JCA_Arkin_RBSFamily}}
false
true
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K299801
1
BBa_K299801
standard biobrick scar (if next sequence does NOT start with ATG)
2010-08-10T11:00:00Z
2015-05-08T01:11:50Z
false
false
_284_419_
0
4959
9
Not in stock
false
false
Anna Olchowik
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K299107
1
BBa_K299107
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)
2010-08-08T11:00:00Z
2015-05-08T01:11:50Z
Anderson's RBS library (original vector).
This is just to obtain accurate sequences while working with Anderson's RBS library in original vector.
false
false
_284_419_
0
4959
9
Not in stock
false
none
false
Anna Olchowik
BBa_K299802
1
BBa_K299802
Aactag - like a biobrick scar but first T changed to A
2010-08-10T11:00:00Z
2015-05-08T01:11:50Z
This is a virtual part useful to anyone working with Anderson's RBS library.
First nucleotide of the biobrick suffix is varies in the library, some RBSes have T, wchich results in a regular suffix, but other hace c,G or A. This Modified scar is to help in sillico assembly of the constructs that use Anderson's RBSes.
false
false
_284_419_
0
4959
9
Not in stock
false
false
Anna Olchowik
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K299000_sequence
1
ttgacggctagctcagtcctaggtacagtgctagcttctagagaaagaggggacaaactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K299801_sequence
1
tactagag
BBa_J61100_sequence
1
aaagaggggaca
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K299802_sequence
1
aactag
BBa_K299107_sequence
1
ttctagag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z