BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_K299804 1 BBa_K299804 Cactag - like a biobrick scar but first T changed to C. 2010-08-10T11:00:00Z 2015-05-08T01:11:50Z Use only if the next part starts with ATG. This is a virtual part useful to anyone working with Anderson's RBS library. First nucleotide of the biobrick suffix is varies in the library. Some RBSes have T, wchich results in a regular suffix, but other hace c,G or A. This modified scar is to help in sillico assembly of the constructs that use Anderson's RBSes. This modified scar is suitable only if the next part starts with ATG false false _284_419_ 0 4959 9 Not in stock false false Anna Olchowik BBa_K299801 1 BBa_K299801 standard biobrick scar (if next sequence does NOT start with ATG) 2010-08-10T11:00:00Z 2015-05-08T01:11:50Z false false _284_419_ 0 4959 9 Not in stock false false Anna Olchowik BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K299026 1 BBa_K299026 RBS measurement device pT7 J61101 GFP 2010-10-15T11:00:00Z 2015-05-08T01:11:50Z This part contains RBS J61101 from the Anderson's collection. In the physical DNA after J61101 in composite parts there is Cactag sequence instead of the biobrick scar Tactag. J61101 was originally designed in a way that its last nucleotide "C" is also first nucleotide of the suffix (wchich should be T)- it modifies the suffix. The sequence of J61101 in the registry does not include its last nucleotide. If it were included the registry sequence would show the additional nucleotide, now it shows T instead of C. The seguence of the J612101 does not match its physical DNA To go around this problem in out bricks we used scarless assembly option and manually included the scars: BBa_K299800 DNA standard biobrick scar (if next part starts with ATG) BBa_K299801 DNA standard biobrick scar (if next sequence does NOT start with ATG) BBa_K299802 DNA Aactag - like a biobrick scar but first T changed to A BBa_K299803 DNA Gactag - like a biobrick scar but first T changed to G BBa_K299804 DNA Cactag - like a biobrick scar but first T changed to C. This brick consists of the following subparts: I719005[T7promoter] BBa_K299801[standard scar] BBa_J61101[RBS] BBa_K299804[Cactag] BBa_E0040[GFP] BBa_K299801[standard scar] BBa_B0010[terminator] BBa_K299801[standard scar] BBa_B0012[terminator] false false _284_419_ 0 4959 9 It's complicated true false Anna Olchowik component2087733 1 BBa_B0012 component2087726 1 BBa_K299804 component2087724 1 BBa_K299801 component2087730 1 BBa_B0010 component2087729 1 BBa_K299801 component2087728 1 BBa_E0040 component2087723 1 BBa_I719005 component2087725 1 BBa_J61101 component2087732 1 BBa_K299801 annotation2087733 1 BBa_B0012 range2087733 1 866 906 annotation2087726 1 BBa_K299804 range2087726 1 44 49 annotation2087728 1 BBa_E0040 range2087728 1 50 769 annotation2087729 1 BBa_K299801 range2087729 1 770 777 annotation2087732 1 BBa_K299801 range2087732 1 858 865 annotation2087725 1 BBa_J61101 range2087725 1 32 43 annotation2087730 1 BBa_B0010 range2087730 1 778 857 annotation2087723 1 BBa_I719005 range2087723 1 1 23 annotation2087724 1 BBa_K299801 range2087724 1 24 31 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K299804_sequence 1 cactag BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K299026_sequence 1 taatacgactcactatagggagatactagagaaagacaggacccactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J61101_sequence 1 aaagacaggacc BBa_K299801_sequence 1 tactagag BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z