BBa_K299201 1 BBa_K299201 J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between** 2010-08-08T11:00:00Z 2015-05-08T01:11:50Z Registry. It is J61101 with part of its original vector. http://partsregistry.org/wiki/index.php?title=Part:BBa_J61101 J23100[promoter].K299107[xbaIG].J61101[RBS] = Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar. Also be careful part J61101 is in registry as AAAGACAGGACCC, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is: AAAGACAGGACCC**a** (and then comes rest of the suffix: ctagt) It means thatThe physical sequence is different than the record in registry for J61101, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway. This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed. The original Anderson RBSes and vector are described here. http://partsregistry.org/wiki/index.php?title=Part:BBa_J61101 false false _284_419_ 0 4959 9 Not in stock false false Anna Olchowik component2076937 1 BBa_J23100 component2076939 1 BBa_J61101 component2076938 1 BBa_K299107 annotation2076938 1 BBa_K299107 range2076938 1 44 51 annotation2076937 1 BBa_J23100 range2076937 1 1 35 annotation2076939 1 BBa_J61101 range2076939 1 60 71 BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K299107 1 BBa_K299107 T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest) 2010-08-08T11:00:00Z 2015-05-08T01:11:50Z Anderson's RBS library (original vector). This is just to obtain accurate sequences while working with Anderson's RBS library in original vector. false false _284_419_ 0 4959 9 Not in stock false none false Anna Olchowik BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_J61101_sequence 1 aaagacaggacc BBa_K299201_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagttctagagtactagagaaagacaggacc BBa_K299107_sequence 1 ttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z