BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
{{JCA_Arkin_RBSFamily}}
false
true
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K299300
1
BBa_K299300
J23100[promoter].K299107[TxbaIG].J61100[RBS] No b-brick scars between
2010-08-08T11:00:00Z
2015-05-08T01:11:50Z
Registry. It is J61100 with part of its original vector.
http://partsregistry.org/wiki/index.php?title=Part:BBa_J61100
J23100[promoter].K299107[xbaIG].J61101[RBS] =
Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
Also be careful part J61100 is in registry as AAAGAGGGGACAA, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:
AAAGAGGGGACAA**a** (and then comes the rest of the suffix: ctagt)
It means that the physical sequence is different to the record in registry for J61100, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.
This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector
with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.
The original Anderson RBSes and vector are described here.
http://partsregistry.org/wiki/index.php?title=Part:BBa_J61100
false
true
_284_419_
0
4959
9
Not in stock
false
false
Anna Olchowik
component2076943
1
BBa_J23100
component2076944
1
BBa_K299107
component2076945
1
BBa_J61100
annotation2076944
1
BBa_K299107
range2076944
1
36
43
annotation2076945
1
BBa_J61100
range2076945
1
44
55
annotation2076943
1
BBa_J23100
range2076943
1
1
35
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K299107
1
BBa_K299107
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)
2010-08-08T11:00:00Z
2015-05-08T01:11:50Z
Anderson's RBS library (original vector).
This is just to obtain accurate sequences while working with Anderson's RBS library in original vector.
false
false
_284_419_
0
4959
9
Not in stock
false
none
false
Anna Olchowik
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_J61100_sequence
1
aaagaggggaca
BBa_K299300_sequence
1
ttgacggctagctcagtcctaggtacagtgctagcttctagagaaagaggggaca
BBa_K299107_sequence
1
ttctagag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z