BBa_K299317
1
BBa_K299317
J23100[promoter].K299107[TxbaIG].J61117[RBS] No b-brick scars between
2010-08-08T11:00:00Z
2015-05-08T01:11:50Z
Registry. It is J61117 with part of its original vector.
http://partsregistry.org/wiki/index.php?title=Part:BBa_J61117
J23100[promoter].K299107[xbaIG].J61107[RBS] =
Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
In this case the physical DNA mathes the registry sequence after assembly part J61117 is in registry as AAAGACATGAGT, The functional DNA of this RBS (part J61117) is AAAGACATGAGT*T*, when suffix is added, the first T of the suffix gives 'AAAGACATGAGTT' sequence.
This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector
with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.
The original Anderson RBSes and vector are described here.
http://partsregistry.org/wiki/index.php?title=Part:BBa_J61117
false
false
_284_419_
0
4959
9
Not in stock
false
false
Anna Olchowik
component2076951
1
BBa_J61117
component2076950
1
BBa_K299107
component2076949
1
BBa_J23100
annotation2076950
1
BBa_K299107
range2076950
1
36
43
annotation2076949
1
BBa_J23100
range2076949
1
1
35
annotation2076951
1
BBa_J61117
range2076951
1
44
55
BBa_J61117
1
BBa_J61117
Ribosome Binding Site Family Member
2007-04-23T11:00:00Z
2015-08-31T01:59:49Z
N/A
{{JCA_Arkin_RBSFamily}}
false
false
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K299107
1
BBa_K299107
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)
2010-08-08T11:00:00Z
2015-05-08T01:11:50Z
Anderson's RBS library (original vector).
This is just to obtain accurate sequences while working with Anderson's RBS library in original vector.
false
false
_284_419_
0
4959
9
Not in stock
false
none
false
Anna Olchowik
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_J61117_sequence
1
aaagacatgagt
BBa_K299107_sequence
1
ttctagag
BBa_K299317_sequence
1
ttgacggctagctcagtcctaggtacagtgctagcttctagagaaagacatgagt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z