BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_I719005
1
pT7
T7 Promoter
2007-10-23T11:00:00Z
2015-08-31T04:07:53Z
---
Released HQ 2013
Just a T7 Promoter
false
false
_128_
0
2097
9
In stock
true
None
true
Imperial 2007
BBa_K299806
1
BBa_K299806
minC cell division inhibitor
2010-10-15T11:00:00Z
2015-05-08T01:11:50Z
Cloned by PCR amplification from E. coli BL21 genome
MinC is a cell division inhibitor. It prevents the FtsZ polymerization and septal ring formation. Thus it inhibits cell division only but leaves other functions (ie. metabolism and cell growth) unchanged.
false
false
_419_
0
7677
9
In stock
true
MinC under too strong RBS and strong promoter inhibits cell divisions in uncontrolled manner. Thus no transformant are generated using following construct: pT7-B0034-minC in BL21.
false
Michał Lower
annotation2087487
1
minC
range2087487
1
1
696
BBa_K299807
1
BBa_K299807
minC under pT7 and B0032
2010-10-15T11:00:00Z
2015-05-08T01:11:50Z
ligation of K299806 and K299507
Construct for testing minC cell division inhibitor protein. This construct was used for MinC performance measurements.
false
false
_419_
0
7677
9
It's complicated
true
false
Michał Lower
component2087493
1
BBa_K299806
component2087488
1
BBa_I719005
component2087490
1
BBa_B0032
annotation2087493
1
BBa_K299806
range2087493
1
51
746
annotation2087490
1
BBa_B0032
range2087490
1
32
44
annotation2087488
1
BBa_I719005
range2087488
1
1
23
BBa_I719005_sequence
1
taatacgactcactatagggaga
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K299806_sequence
1
atgtcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaacctaaggttatccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaacgtcagtgcactggaagacccggtaaactggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggcgtaagtggctgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaaaagcgccacgtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagataccccggtgcgttccggtcagcgtatttatgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaattgattgccgatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaacgcaaatattttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagcagaattttatggcaaagcggcgcgactgcagttagtcgaaaacgctttgaccgttcaaccgttaaattga
BBa_K299807_sequence
1
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgtcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaacctaaggttatccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaacgtcagtgcactggaagacccggtaaactggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggcgtaagtggctgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaaaagcgccacgtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagataccccggtgcgttccggtcagcgtatttatgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaattgattgccgatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaacgcaaatattttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagcagaattttatggcaaagcggcgcgactgcagttagtcgaaaacgctttgaccgttcaaccgttaaattga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z