BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_K299811
1
BBa_K299811
B0032 Listeriolysin
2010-10-12T11:00:00Z
2015-05-08T01:11:50Z
false
false
_419_
0
7677
9
It's complicated
true
false
Michał Lower
component2085760
1
BBa_B0032
component2085763
1
BBa_K177026
annotation2085760
1
BBa_B0032
range2085760
1
1
13
annotation2085763
1
BBa_K177026
range2085763
1
20
1609
BBa_K177026
1
BBa_K177026
listeriolysin O 399G->C - locus from Listeria monocytogenes
2009-07-13T11:00:00Z
2015-05-08T01:11:05Z
Listeria monocytogenes
Hemolytic protein - enables escape from endosome of a mammalian cell, following endocytosis.
false
false
_284_
0
4696
9
It's complicated
true
sequence after mutagenesis: 399G->C
false
Kamila Ornoch
annotation2011266
1
protein
range2011266
1
1
1590
BBa_K299811_sequence
1
tcacacaggaaagtactagatgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataa
BBa_K177026_sequence
1
atgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataa
BBa_B0032_sequence
1
tcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z