LOCUS BBa_K299813 5189 bp DNA linear 05-Nov-2024 DEFINITION AraC regulated cell invasion device ACCESSION BBa_K299813 VERSION BBa_K299813.1 FEATURES Location/Qualifiers misc_feature 4..29 /label=c-amp2 misc_feature 6..44 /label=araO1 misc_feature 43..72 /label=c-amp1 misc_feature 73..101 /label=ara1 and ara2 promoter 113..118 /label=-35 promoter 136..141 /label=-10 promoter 1..149 /label=BBa_R0080 RBS 158..170 /label=RBS-3\Weak RBS 164..167 /label=RBS misc_feature 158..170 /label=BBa_B0032 RBS 158..170 /label=BBa_B0032 CDS 179..3004 /label=protein CDS 179..2686 /label=BBa_K177010 RBS 2695..2707 /label=RBS-3\Weak RBS 2701..2704 /label=RBS misc_feature 2695..2707 /label=BBa_B0032 RBS 2695..2707 /label=BBa_B0032 CDS 2714..4303 /label=protein CDS 2714..4303 /label=BBa_K177026 RBS 4312..4326 /label=RBS-1\Strong RBS 4319..4323 /label=RBS misc_feature 4312..4326 /label=BBa_B0030 RBS 4312..4326 /label=BBa_B0030 CDS 4333..5052 /label=GFP protein CDS 4333..5052 /label=BBa_E0040 stem_loop 5072..5115 /label=stem_loop misc_feature 5061..5140 /label=BBa_B0010 terminator 5061..5140 /label=BBa_B0010 stem_loop 5156..5175 /label=T7 TE misc_feature 5182..5182 /label=stop polyA_site 5176..5189 /label=polya misc_feature 5149..5189 /label=BBa_B0012 terminator 5149..5189 /label=BBa_B0012 ORIGIN 1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt 61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt 121 tttatcgcaa ctctctactg tttctccatt actagagtca cacaggaaag tactagagct 181 attgaggctc cgcacacagc ggatacgccg tagccgcaga tgttggtatg tcgccggtgg 241 tcatatccat agtgacaaaa tctgtactgg tctttttagt ccaatagtta cccgatggcc 301 actcagcgct atcataggtt gccaaacttc cccactctcc ccacagagtc ccgtctggtg 361 aacgcgaacc attactggcg cgtgcggact cgatcagcgc agtaaaatca gtgcgctggc 421 aatttcgact cgcctcgaca cttgattgca gtgacatggt gccggagaac acccacaaat 481 taggtttgaa ttgatatgtt gccgtataac tcgggaattt tttacttttt gccgtcacag 541 tgacggtgct accataggtt ttataggcaa tattgacttt accctgatta tcaaccggcg 601 cactggccgc ataggatgat gtccagtcat attgagtatt attcgccaca tcgtcattca 661 tcaccaattg gaacgtggct ttattaaagg tagttttcgg gaagccttta tctgtggcaa 721 attgctcacc attcacctta atgccggtta tcttcggtac tgggtacagg gtgattcttt 781 tctgcaacaa gtctagtgat tcccccccca cctgcggcgt aatatcgaca tctcctacgg 841 aatttcccac cacactggcg gtatagttgt cagcattttc ggttaccgaa ctaatagtta 901 ccggaacacc actttgtata aattcaagat ctgttatccc actgacaaac tcattatttt 961 tattacgcgg aacaaaggtg agtatcgact gcatggagcc atcggcaaca atatcagatg 1021 gagaaacgtt aaaacttgag tttgtgacat ctggcggcag cacggcaaat cgtacatcaa 1081 cagtagcctg ttgcccctga atatctaaag tgacgactga caccccaaca gtgaagctag 1141 ttaatgcaat gcttatcaca ccctgtgcat cggttttttt tgtgacttta ctgggaagcg 1201 caccattact ggtggttatc accccctctt gctcggcaac cggcgtacta tcaatattag 1261 tcactgtaaa tttcaccgtg atgggagtcc tgccatcagc aagcgcacca tcatcagtca 1321 cttccgaggt aataacgaac tgaggttgct gcacaataac ggtcaacgta gctggattag 1381 aactattgcc gtggttatcg atagccgtgg cgctgagggt ataggtattg accgccgctt 1441 cgctctcgcc ggttttcccc acagtacgac tcgctggtgc catcggttta taaggcggta 1501 agaccacatt gtaatctgtt gcactgacgg gtattaattt gccgccggca gcaacccatt 1561 gcgcatcatt ccagagaata cgttgtagtg cagattgaga ctgtatttgt gcactaacgg 1621 aataaacctg ccccggcagg ccggagagta cggcgggtga aaatgccaat ttgatagtat 1681 tctgtttttg gtattccaga acaatatttg gattgcgctc aaccagatta taacggctct 1741 cagccagtaa acgagttccg gccaccactg cggggctaaa ctgcgaacga aaactttcgc 1801 cgaggcgata atccatttgt aagttccatt gtatttcatg ctgccgactt tttcccattc 1861 gttgatcgac ccccagtgta atgaagggga tcggcgtata aataagccct gtggtcaccg 1921 cataagggtt actttgcagg ttatctttac caaataaagc cacccgctca ccacggtact 1981 gctcatattt taatttcccg cccaattgtg gcagcgcagg taaatacgct ttgacgtgaa 2041 tgtcgccccc gcttgccggg cgctcattat agtccgcgaa atcacgagat tgatgccaac 2101 cattgaggcg aaaataacca ttggccgata attgtaaata atcagtccag gcttctgcac 2161 ccacgccaat acgatgattg tgcccggtca tatcattgtc atagaaagtg ttaaagccat 2221 acatccaact ttgttggaag gtacgcaccc cagcgccgat attaagggta ttgcgactgt 2281 ctttatttct tatacccaac tgactaaaaa agacatatga agcagagtca taccaaggca 2341 acagccaatc aagagaactt tctttgaggg agaacttttt atcaaagtta acattgacct 2401 gagttgtccc aaatctattt aaccagtgtt ttacttcctg atttgccgcg tcatttacca 2461 ttgacctggt gatattggat gcattacccg ataccgccag actggttccg gtactgatta 2521 tcttattgaa agcctcatta tgttcagcgg ggttctctaa cgcttctgaa ttaaaataat 2581 gttgctgtga gaacccataa gtaaatattc caaatattaa accgatcgat aatattagcc 2641 tgctaattat tttagtcaca gttagcgtat taaaaaatga atacattact agagtcacac 2701 aggaaagtac tagatgaaaa aaataatgct agtttttatt acacttatat tagttagtct 2761 accaattgcg caacaaactg aagcaaagga tgcatctgca ttcaataaag aaaatttaat 2821 ttcatccata gcaccaccag catctccgcc tgcaagtcct aagacgccaa tcgaaaagaa 2881 acacgcggat gaaatcgata agtatataca aggattggat tacaataaaa acaatgtatt 2941 agtataccac ggagatgcag tgacaaatgt gccgccaaga aaaggttata aagatggaaa 3001 tgaatatatc gttgtggaga aaaagaagaa atccatcaat caaaataatg cagatatcca 3061 agttgtgaat gcaatttcga gcctaacata tccaggtgct ctcgtgaaag ccaattcgga 3121 attagtagaa aatcaacccg atgttcttcc tgtcaaacgt gattcattaa cacttagcat 3181 tgatttgcca ggaatgacta atcaagacaa taaaattgtt gtaaaaaatg ctactaaatc 3241 gaacgttaac aacgcagtaa atacattagt ggaaagatgg aatgaaaaat atgctcaagc 3301 ttatccaaat gtaagtgcaa aaattgatta tgatgacgaa atggcttaca gtgaatcaca 3361 attaattgca aaatttggta cggcatttaa agctgtaaat aatagcttga atgtaaactt 3421 cggcgcaatc agtgaaggga aaatgcaaga agaagtcatt agttttaaac aaatttacta 3481 taacgtgaat gttaatgaac ctacaagacc ttccagattt ttcggcaaag ctgttactaa 3541 agagcagttg caagcgcttg gagtgaatgc agaaaatcct cctgcatata tctcaagtgt 3601 ggcatatggc cgtcaagttt atttgaaatt atcaactaat tcccatagta ccaaagtaaa 3661 agctgctttt gacgctgccg taagtgggaa atctgtctca ggtgatgtag aactgacaaa 3721 tatcatcaaa aattcttcct tcaaagccgt aatttacggt ggctccgcaa aagatgaagt 3781 tcaaatcatc gacggtaacc tcggagactt acgagatatt ttgaaaaaag gtgctacttt 3841 taaccgggaa acaccaggag ttcccattgc ctatacaaca aacttcttaa aagacaatga 3901 attagctgtt attaaaaaca actcagaata tattgaaaca acttcaaaag cttatacaga 3961 tggaaaaatc aacatcgatc actctggagg atacgttgct caattcaaca tctcttggga 4021 tgaaataaat tatgatcctg aaggtaacga aattgttcaa cataaaaact ggagcgaaaa 4081 caataaaagt aagctagctc atttcacatc gtccatctat ttgccaggta acgcaagaaa 4141 tattaatgtt tacgctaaag aatgcactgg tttagcttgg gaatggtgga gaacggtaat 4201 tgatgaccgg aacctaccgc ttgtgaaaaa tagaaatatc tccatctggg gcactacact 4261 ttatccgaaa tatagtaata gtgtagataa tccaatcgaa taatactaga gattaaagag 4321 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 4381 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 4441 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 4501 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat 4561 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga 4621 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt 4681 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt 4741 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa 4801 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt 4861 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca 4921 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac 4981 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 5041 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 5101 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 5161 accttcgggt gggcctttct gcgtttata //