BBa_K299813
type: ComponentDefinition
persistent identity: BBa K299813
display id: BBa_K299813
version: 1
was generated by: iGEM to SBOL conversion
description: AraC regulated cell invasion device
created: 2010-10-12T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _419_
m user id: 0
owner id: 7677
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Michał Lower
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0040
component: BBa_B0030
component: BBa_K177026
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
component: BBa_K177010
component: BBa_R0080
sequence annotation: BBa_R0080
sequence annotation: BBa_B0032
sequence annotation: BBa_K177010
sequence annotation: BBa_K177026
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K299813_sequence/1

BBa_E0040
type: Component
persistent identity: component2085833
display id: component2085833
version: 1
top level: BBa_K299813
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2085830
display id: component2085830
version: 1
top level: BBa_K299813
definition: BBa_B0030
access: public

BBa_K177026
type: Component
persistent identity: component2085828
display id: component2085828
version: 1
top level: BBa_K299813
definition: BBa_K177026
access: public

BBa_B0032
type: Component
persistent identity: component2085825
display id: component2085825
version: 1
top level: BBa_K299813
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component2085836
display id: component2085836
version: 1
top level: BBa_K299813
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2085834
display id: component2085834
version: 1
top level: BBa_K299813
definition: BBa_B0010
access: public

BBa_K177010
type: Component
persistent identity: component2085823
display id: component2085823
version: 1
top level: BBa_K299813
definition: BBa_K177010
access: public

BBa_B0032
type: Component
persistent identity: component2085820
display id: component2085820
version: 1
top level: BBa_K299813
definition: BBa_B0032
access: public

BBa_R0080
type: Component
persistent identity: component2085818
display id: component2085818
version: 1
top level: BBa_K299813
definition: AraC
access: public

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2085818
display id: annotation2085818
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085818/range2085818/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K299813/annotation2085818/range2085818/1
type: Range
persistent identity: range2085818
display id: range2085818
version: 1
top level: BBa_K299813
direction: direction/reverse
start: 1
end: 149
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2085820
display id: annotation2085820
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085820/range2085820/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K299813/annotation2085820/range2085820/1
type: Range
persistent identity: range2085820
display id: range2085820
version: 1
top level: BBa_K299813
direction: direction/forward
start: 158
end: 170
orientation: inline

BBa_K177010
type: SequenceAnnotation
persistent identity: annotation2085823
display id: annotation2085823
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085823/range2085823/1
component: BBa_K177010

https://synbiohub.org/public/igem/BBa_K299813/annotation2085823/range2085823/1
type: Range
persistent identity: range2085823
display id: range2085823
version: 1
top level: BBa_K299813
direction: direction/reverse
start: 179
end: 2686
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2085825
display id: annotation2085825
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085825/range2085825/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K299813/annotation2085825/range2085825/1
type: Range
persistent identity: range2085825
display id: range2085825
version: 1
top level: BBa_K299813
direction: direction/forward
start: 2695
end: 2707
orientation: inline

BBa_K177026
type: SequenceAnnotation
persistent identity: annotation2085828
display id: annotation2085828
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085828/range2085828/1
component: BBa_K177026

https://synbiohub.org/public/igem/BBa_K299813/annotation2085828/range2085828/1
type: Range
persistent identity: range2085828
display id: range2085828
version: 1
top level: BBa_K299813
direction: direction/reverse
start: 2714
end: 4303
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2085830
display id: annotation2085830
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085830/range2085830/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K299813/annotation2085830/range2085830/1
type: Range
persistent identity: range2085830
display id: range2085830
version: 1
top level: BBa_K299813
direction: direction/forward
start: 4312
end: 4326
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2085833
display id: annotation2085833
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085833/range2085833/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K299813/annotation2085833/range2085833/1
type: Range
persistent identity: range2085833
display id: range2085833
version: 1
top level: BBa_K299813
direction: direction/reverse
start: 4333
end: 5052
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085834
display id: annotation2085834
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085834/range2085834/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K299813/annotation2085834/range2085834/1
type: Range
persistent identity: range2085834
display id: range2085834
version: 1
top level: BBa_K299813
direction: direction/forward
start: 5061
end: 5140
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085836
display id: annotation2085836
version: 1
top level: BBa_K299813
location: https://synbiohub.org/public/igem/BBa_K299813/annotation2085836/range2085836/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K299813/annotation2085836/range2085836/1
type: Range
persistent identity: range2085836
display id: range2085836
version: 1
top level: BBa_K299813
direction: direction/forward
start: 5149
end: 5189
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

BBa_K177010
type: ComponentDefinition
persistent identity: BBa K177010
display id: BBa_K177010
version: 1
was generated by: iGEM to SBOL conversion
description: invasine locus from yersinia
created: 2009-07-06T11:00:00Z
modified: 2015-05-08T01:11:05Z
mutable provenance: Genomic DNA from Yersinia
owned by: james
owned by: myers
mutable description: enable bacteria to enter a cell
discontinued: false
dominant: false
group u list: _284_
m user id: 0
owner id: 4696
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Kamila Ornoch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K177010_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2010434
display id: annotation2010434
version: 1
top level: BBa_K177010
location: https://synbiohub.org/public/igem/BBa_K177010/annotation2010434/range2010434/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K177010/annotation2010434/range2010434/1
type: Range
persistent identity: range2010434
display id: range2010434
version: 1
top level: BBa_K177010
direction: direction/reverse
start: 1
end: 2826
orientation: inline

BBa_K177026
type: ComponentDefinition
persistent identity: BBa K177026
display id: BBa_K177026
version: 1
was generated by: iGEM to SBOL conversion
description: listeriolysin O 399G->C - locus from Listeria monocytogenes
created: 2009-07-13T11:00:00Z
modified: 2015-05-08T01:11:05Z
mutable provenance: Listeria monocytogenes
owned by: james
owned by: myers
mutable description: Hemolytic protein - enables escape from endosome of a mammalian cell, following endocytosis.
discontinued: false
dominant: false
experience: experience/Works
group u list: _284_
m user id: 0
owner id: 4696
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: sequence after mutagenesis: 399G->C
star: false
creator: Kamila Ornoch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K177026_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2011266
display id: annotation2011266
version: 1
top level: BBa_K177026
location: https://synbiohub.org/public/igem/BBa_K177026/annotation2011266/range2011266/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K177026/annotation2011266/range2011266/1
type: Range
persistent identity: range2011266
display id: range2011266
version: 1
top level: BBa_K177026
direction: direction/forward
start: 1
end: 1590
orientation: inline

https://synbiohub.org/public/igem/BBa_K299813_sequence/1
type: Sequence
persistent identity: BBa K299813 sequence
display id: BBa_K299813_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagtcacacaggaaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K177010_sequence/1
type: Sequence
persistent identity: BBa K177010 sequence
display id: BBa_K177010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K177026_sequence/1
type: Sequence
persistent identity: BBa K177026 sequence
display id: BBa_K177026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z