LOCUS BBa_K299815 5075 bp DNA linear 08-Feb-2026 DEFINITION invasion device constitutively expressed ACCESSION BBa_K299815 VERSION BBa_K299815.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23102 RBS 44..56 /label=RBS-3\Weak RBS 50..53 /label=RBS misc_feature 44..56 /label=BBa_B0032 RBS 44..56 /label=BBa_B0032 CDS 65..2890 /label=protein CDS 65..2572 /label=BBa_K177010 RBS 2581..2593 /label=RBS-3\Weak RBS 2587..2590 /label=RBS misc_feature 2581..2593 /label=BBa_B0032 RBS 2581..2593 /label=BBa_B0032 CDS 2600..4189 /label=protein CDS 2600..4189 /label=BBa_K177026 RBS 4198..4212 /label=RBS-1\Strong RBS 4205..4209 /label=RBS misc_feature 4198..4212 /label=BBa_B0030 RBS 4198..4212 /label=BBa_B0030 CDS 4219..4938 /label=GFP protein CDS 4219..4938 /label=BBa_E0040 stem_loop 4958..5001 /label=stem_loop misc_feature 4947..5026 /label=BBa_B0010 terminator 4947..5026 /label=BBa_B0010 stem_loop 5042..5061 /label=T7 TE misc_feature 5068..5068 /label=stop polyA_site 5062..5075 /label=polya misc_feature 5035..5075 /label=BBa_B0012 terminator 5035..5075 /label=BBa_B0012 ORIGIN 1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagtcacaca ggaaagtact 61 agagctattg aggctccgca cacagcggat acgccgtagc cgcagatgtt ggtatgtcgc 121 cggtggtcat atccatagtg acaaaatctg tactggtctt tttagtccaa tagttacccg 181 atggccactc agcgctatca taggttgcca aacttcccca ctctccccac agagtcccgt 241 ctggtgaacg cgaaccatta ctggcgcgtg cggactcgat cagcgcagta aaatcagtgc 301 gctggcaatt tcgactcgcc tcgacacttg attgcagtga catggtgccg gagaacaccc 361 acaaattagg tttgaattga tatgttgccg tataactcgg gaatttttta ctttttgccg 421 tcacagtgac ggtgctacca taggttttat aggcaatatt gactttaccc tgattatcaa 481 ccggcgcact ggccgcatag gatgatgtcc agtcatattg agtattattc gccacatcgt 541 cattcatcac caattggaac gtggctttat taaaggtagt tttcgggaag cctttatctg 601 tggcaaattg ctcaccattc accttaatgc cggttatctt cggtactggg tacagggtga 661 ttcttttctg caacaagtct agtgattccc cccccacctg cggcgtaata tcgacatctc 721 ctacggaatt tcccaccaca ctggcggtat agttgtcagc attttcggtt accgaactaa 781 tagttaccgg aacaccactt tgtataaatt caagatctgt tatcccactg acaaactcat 841 tatttttatt acgcggaaca aaggtgagta tcgactgcat ggagccatcg gcaacaatat 901 cagatggaga aacgttaaaa cttgagtttg tgacatctgg cggcagcacg gcaaatcgta 961 catcaacagt agcctgttgc ccctgaatat ctaaagtgac gactgacacc ccaacagtga 1021 agctagttaa tgcaatgctt atcacaccct gtgcatcggt tttttttgtg actttactgg 1081 gaagcgcacc attactggtg gttatcaccc cctcttgctc ggcaaccggc gtactatcaa 1141 tattagtcac tgtaaatttc accgtgatgg gagtcctgcc atcagcaagc gcaccatcat 1201 cagtcacttc cgaggtaata acgaactgag gttgctgcac aataacggtc aacgtagctg 1261 gattagaact attgccgtgg ttatcgatag ccgtggcgct gagggtatag gtattgaccg 1321 ccgcttcgct ctcgccggtt ttccccacag tacgactcgc tggtgccatc ggtttataag 1381 gcggtaagac cacattgtaa tctgttgcac tgacgggtat taatttgccg ccggcagcaa 1441 cccattgcgc atcattccag agaatacgtt gtagtgcaga ttgagactgt atttgtgcac 1501 taacggaata aacctgcccc ggcaggccgg agagtacggc gggtgaaaat gccaatttga 1561 tagtattctg tttttggtat tccagaacaa tatttggatt gcgctcaacc agattataac 1621 ggctctcagc cagtaaacga gttccggcca ccactgcggg gctaaactgc gaacgaaaac 1681 tttcgccgag gcgataatcc atttgtaagt tccattgtat ttcatgctgc cgactttttc 1741 ccattcgttg atcgaccccc agtgtaatga aggggatcgg cgtataaata agccctgtgg 1801 tcaccgcata agggttactt tgcaggttat ctttaccaaa taaagccacc cgctcaccac 1861 ggtactgctc atattttaat ttcccgccca attgtggcag cgcaggtaaa tacgctttga 1921 cgtgaatgtc gcccccgctt gccgggcgct cattatagtc cgcgaaatca cgagattgat 1981 gccaaccatt gaggcgaaaa taaccattgg ccgataattg taaataatca gtccaggctt 2041 ctgcacccac gccaatacga tgattgtgcc cggtcatatc attgtcatag aaagtgttaa 2101 agccatacat ccaactttgt tggaaggtac gcaccccagc gccgatatta agggtattgc 2161 gactgtcttt atttcttata cccaactgac taaaaaagac atatgaagca gagtcatacc 2221 aaggcaacag ccaatcaaga gaactttctt tgagggagaa ctttttatca aagttaacat 2281 tgacctgagt tgtcccaaat ctatttaacc agtgttttac ttcctgattt gccgcgtcat 2341 ttaccattga cctggtgata ttggatgcat tacccgatac cgccagactg gttccggtac 2401 tgattatctt attgaaagcc tcattatgtt cagcggggtt ctctaacgct tctgaattaa 2461 aataatgttg ctgtgagaac ccataagtaa atattccaaa tattaaaccg atcgataata 2521 ttagcctgct aattatttta gtcacagtta gcgtattaaa aaatgaatac attactagag 2581 tcacacagga aagtactaga tgaaaaaaat aatgctagtt tttattacac ttatattagt 2641 tagtctacca attgcgcaac aaactgaagc aaaggatgca tctgcattca ataaagaaaa 2701 tttaatttca tccatagcac caccagcatc tccgcctgca agtcctaaga cgccaatcga 2761 aaagaaacac gcggatgaaa tcgataagta tatacaagga ttggattaca ataaaaacaa 2821 tgtattagta taccacggag atgcagtgac aaatgtgccg ccaagaaaag gttataaaga 2881 tggaaatgaa tatatcgttg tggagaaaaa gaagaaatcc atcaatcaaa ataatgcaga 2941 tatccaagtt gtgaatgcaa tttcgagcct aacatatcca ggtgctctcg tgaaagccaa 3001 ttcggaatta gtagaaaatc aacccgatgt tcttcctgtc aaacgtgatt cattaacact 3061 tagcattgat ttgccaggaa tgactaatca agacaataaa attgttgtaa aaaatgctac 3121 taaatcgaac gttaacaacg cagtaaatac attagtggaa agatggaatg aaaaatatgc 3181 tcaagcttat ccaaatgtaa gtgcaaaaat tgattatgat gacgaaatgg cttacagtga 3241 atcacaatta attgcaaaat ttggtacggc atttaaagct gtaaataata gcttgaatgt 3301 aaacttcggc gcaatcagtg aagggaaaat gcaagaagaa gtcattagtt ttaaacaaat 3361 ttactataac gtgaatgtta atgaacctac aagaccttcc agatttttcg gcaaagctgt 3421 tactaaagag cagttgcaag cgcttggagt gaatgcagaa aatcctcctg catatatctc 3481 aagtgtggca tatggccgtc aagtttattt gaaattatca actaattccc atagtaccaa 3541 agtaaaagct gcttttgacg ctgccgtaag tgggaaatct gtctcaggtg atgtagaact 3601 gacaaatatc atcaaaaatt cttccttcaa agccgtaatt tacggtggct ccgcaaaaga 3661 tgaagttcaa atcatcgacg gtaacctcgg agacttacga gatattttga aaaaaggtgc 3721 tacttttaac cgggaaacac caggagttcc cattgcctat acaacaaact tcttaaaaga 3781 caatgaatta gctgttatta aaaacaactc agaatatatt gaaacaactt caaaagctta 3841 tacagatgga aaaatcaaca tcgatcactc tggaggatac gttgctcaat tcaacatctc 3901 ttgggatgaa ataaattatg atcctgaagg taacgaaatt gttcaacata aaaactggag 3961 cgaaaacaat aaaagtaagc tagctcattt cacatcgtcc atctatttgc caggtaacgc 4021 aagaaatatt aatgtttacg ctaaagaatg cactggttta gcttgggaat ggtggagaac 4081 ggtaattgat gaccggaacc taccgcttgt gaaaaataga aatatctcca tctggggcac 4141 tacactttat ccgaaatata gtaatagtgt agataatcca atcgaataat actagagatt 4201 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 4261 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 4321 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 4381 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 4441 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 4501 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 4561 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 4621 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 4681 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 4741 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 4801 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 4861 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 4921 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 4981 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 5041 tggctcacct tcgggtgggc ctttctgcgt ttata //