LOCUS       BBa_K299816             3458 bp    DNA     linear       22-Nov-2024
DEFINITION  control construct - invasin + GFP under J23102
ACCESSION   BBa_K299816
VERSION     BBa_K299816.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             65..2890
                     /label=protein
     CDS             65..2572
                     /label=BBa_K177010
     RBS             2581..2595
                     /label=RBS-1\Strong
     RBS             2588..2592
                     /label=RBS
     misc_feature    2581..2595
                     /label=BBa_B0030
     RBS             2581..2595
                     /label=BBa_B0030
     CDS             2602..3321
                     /label=GFP protein
     CDS             2602..3321
                     /label=BBa_E0040
     stem_loop       3341..3384
                     /label=stem_loop
     misc_feature    3330..3409
                     /label=BBa_B0010
     terminator      3330..3409
                     /label=BBa_B0010
     stem_loop       3425..3444
                     /label=T7 TE
     misc_feature    3451..3451
                     /label=stop
     polyA_site      3445..3458
                     /label=polya
     misc_feature    3418..3458
                     /label=BBa_B0012
     terminator      3418..3458
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagtcacaca ggaaagtact
       61 agagctattg aggctccgca cacagcggat acgccgtagc cgcagatgtt ggtatgtcgc
      121 cggtggtcat atccatagtg acaaaatctg tactggtctt tttagtccaa tagttacccg
      181 atggccactc agcgctatca taggttgcca aacttcccca ctctccccac agagtcccgt
      241 ctggtgaacg cgaaccatta ctggcgcgtg cggactcgat cagcgcagta aaatcagtgc
      301 gctggcaatt tcgactcgcc tcgacacttg attgcagtga catggtgccg gagaacaccc
      361 acaaattagg tttgaattga tatgttgccg tataactcgg gaatttttta ctttttgccg
      421 tcacagtgac ggtgctacca taggttttat aggcaatatt gactttaccc tgattatcaa
      481 ccggcgcact ggccgcatag gatgatgtcc agtcatattg agtattattc gccacatcgt
      541 cattcatcac caattggaac gtggctttat taaaggtagt tttcgggaag cctttatctg
      601 tggcaaattg ctcaccattc accttaatgc cggttatctt cggtactggg tacagggtga
      661 ttcttttctg caacaagtct agtgattccc cccccacctg cggcgtaata tcgacatctc
      721 ctacggaatt tcccaccaca ctggcggtat agttgtcagc attttcggtt accgaactaa
      781 tagttaccgg aacaccactt tgtataaatt caagatctgt tatcccactg acaaactcat
      841 tatttttatt acgcggaaca aaggtgagta tcgactgcat ggagccatcg gcaacaatat
      901 cagatggaga aacgttaaaa cttgagtttg tgacatctgg cggcagcacg gcaaatcgta
      961 catcaacagt agcctgttgc ccctgaatat ctaaagtgac gactgacacc ccaacagtga
     1021 agctagttaa tgcaatgctt atcacaccct gtgcatcggt tttttttgtg actttactgg
     1081 gaagcgcacc attactggtg gttatcaccc cctcttgctc ggcaaccggc gtactatcaa
     1141 tattagtcac tgtaaatttc accgtgatgg gagtcctgcc atcagcaagc gcaccatcat
     1201 cagtcacttc cgaggtaata acgaactgag gttgctgcac aataacggtc aacgtagctg
     1261 gattagaact attgccgtgg ttatcgatag ccgtggcgct gagggtatag gtattgaccg
     1321 ccgcttcgct ctcgccggtt ttccccacag tacgactcgc tggtgccatc ggtttataag
     1381 gcggtaagac cacattgtaa tctgttgcac tgacgggtat taatttgccg ccggcagcaa
     1441 cccattgcgc atcattccag agaatacgtt gtagtgcaga ttgagactgt atttgtgcac
     1501 taacggaata aacctgcccc ggcaggccgg agagtacggc gggtgaaaat gccaatttga
     1561 tagtattctg tttttggtat tccagaacaa tatttggatt gcgctcaacc agattataac
     1621 ggctctcagc cagtaaacga gttccggcca ccactgcggg gctaaactgc gaacgaaaac
     1681 tttcgccgag gcgataatcc atttgtaagt tccattgtat ttcatgctgc cgactttttc
     1741 ccattcgttg atcgaccccc agtgtaatga aggggatcgg cgtataaata agccctgtgg
     1801 tcaccgcata agggttactt tgcaggttat ctttaccaaa taaagccacc cgctcaccac
     1861 ggtactgctc atattttaat ttcccgccca attgtggcag cgcaggtaaa tacgctttga
     1921 cgtgaatgtc gcccccgctt gccgggcgct cattatagtc cgcgaaatca cgagattgat
     1981 gccaaccatt gaggcgaaaa taaccattgg ccgataattg taaataatca gtccaggctt
     2041 ctgcacccac gccaatacga tgattgtgcc cggtcatatc attgtcatag aaagtgttaa
     2101 agccatacat ccaactttgt tggaaggtac gcaccccagc gccgatatta agggtattgc
     2161 gactgtcttt atttcttata cccaactgac taaaaaagac atatgaagca gagtcatacc
     2221 aaggcaacag ccaatcaaga gaactttctt tgagggagaa ctttttatca aagttaacat
     2281 tgacctgagt tgtcccaaat ctatttaacc agtgttttac ttcctgattt gccgcgtcat
     2341 ttaccattga cctggtgata ttggatgcat tacccgatac cgccagactg gttccggtac
     2401 tgattatctt attgaaagcc tcattatgtt cagcggggtt ctctaacgct tctgaattaa
     2461 aataatgttg ctgtgagaac ccataagtaa atattccaaa tattaaaccg atcgataata
     2521 ttagcctgct aattatttta gtcacagtta gcgtattaaa aaatgaatac attactagag
     2581 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
     2641 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     2701 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     2761 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     2821 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     2881 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     2941 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     3001 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     3061 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     3121 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     3181 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     3241 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     3301 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     3361 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3421 cactggctca ccttcgggtg ggcctttctg cgtttata
//