LOCUS       BBa_K300000             2171 bp    DNA     linear       25-Nov-2024
DEFINITION  BioBrick integrative base vector for E. coli
ACCESSION   BBa_K300000
VERSION     BBa_K300000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Suffix
     misc_feature    2..7
                     /label=SpeI
     misc_feature    16..21
                     /label=PstI
     misc_feature    9..16
                     /label=NotI
     misc_feature    1..21
                     /label=BBa_G00001
     stem_loop       32..63
                     /label=stem loop
     misc_feature    25..64
                     /label=identical to E. coli MG1655 between tonB and yciA
     terminator      22..90
                     /label=BBa_B0054
     primer_bind     91..110
                     /label=VR
     misc_feature    91..110
                     /label=BBa_G00102
     stem_loop       124..143
                     /label=stem_loop
     terminator      111..151
                     /label=BBa_B0062
     misc_feature    152..187
                     /label=BBa_J72001
     misc_feature    188..194
                     /label=BBa_K300994
     misc_feature    195..600
                     /label=BBa_K300998
     misc_feature    601..608
                     /label=BBa_G0001
     misc_feature    609..649
                     /label=B0012
     misc_feature    658..737
                     /label=B0010
     terminator      609..737
                     /label=BBa_B0025
     misc_feature    738..745
                     /label=BBa_G0001
     CDS             746..1408
                     /label=cmR(rev)
     misc_feature    746..1514
                     /label=BBa_K300999
     misc_feature    1515..1521
                     /label=BBa_K300995
     misc_feature    1522..1557
                     /label=BBa_J72001
     misc_feature    1558..1563
                     /label=NheI site
     misc_feature    1558..1563
                     /label=BBa_B0045
     misc_feature    1564..1973
                     /label=BBa_K300991
     misc_feature    1974..1979
                     /label=NheI site
     misc_feature    1974..1979
                     /label=BBa_B0045
     stem_loop       1988..2022
                     /label=stem_loop
     terminator      1980..2051
                     /label=BBa_B0053
     primer_bind     2052..2071
                     /label=VF2
     primer          2052..2071
                     /label=BBa_G00100
     stem_loop       2107..2128
                     /label=stem loop from bacteriophage T3
     misc_feature    2099..2137
                     /label=identical to bacteriophage T3
     terminator      2072..2149
                     /label=BBa_B0055
     misc_feature    2150..2155
                     /label=EcoRI site
     misc_feature    2165..2170
                     /label=XbaI site
     misc_feature    2150..2171
                     /label=Prefix
     misc_feature    2156..2163
                     /label=NotI site
     misc_feature    2150..2171
                     /label=BBa_G00000
ORIGIN
        1 tactagtagc ggccgctgca gattagcaga aagtcaaaag cctccgaccg gaggcttttg
       61 actaaaactt cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa
      121 aaatccttag ctttcgctaa ggatgatttc tgcgaagttc ctatactttc tagagaatag
      181 gaacttccct agtagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg
      241 tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg
      301 ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc
      361 tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa
      421 gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt
      481 ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt
      541 gagtgacaca ggaacactta acggctgaca tgggaattag ccatgggccc gtgcgaatca
      601 ctctagtata taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag
      661 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
      721 ttttatttga tgcctggctc tagtattatt acgccccgcc ctgccactca tcacagtact
      781 gttgtaattc attaagcatg cggccgacat ggaagccatc acaaacggca tgatgaacct
      841 ggatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atcgtgaaaa
      901 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
      961 agggattggc tgagacaaaa aacatattct caataaaccc tttagggaaa taggccaggt
     1021 tttcaccgta acacgccaca tcttgcgaat atatgtggag aaactgccgg aaatcgtcgt
     1081 ggtattcact ccagagggac gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     1141 ggtgaacact atcccagatc accagctcac cgtctttcat ggccatacga aactccgggt
     1201 gagcgttcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     1261 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     1321 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     1381 tggtatatcc cgtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     1441 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1501 cgtgcccgat caacctctag ggcgaagttc ctatactttc tagagaatag gaacttcgct
     1561 agccatgggc ccgtgcgaat cagaaataat ctcgatatca tctcttttct tgccgagcgc
     1621 cttcctgagc tttgtgtcgg tgatcattga atgggtacac atttttgttt ttgggtacac
     1681 aaaagtgtac acaaagttgc ccactcaaag ctacacgcaa tgtaacacta gttcgcagag
     1741 tgttatggtt tacatccttg aaagcctgct ggataagggt ttagcgtaac agaacgtttt
     1801 tacgcggaat tgttcgtaat atgccaaatg acaatttaag aaagtgttct aatttattag
     1861 aaatttgcac ttaaatcaaa aagttacgga caattcaacc accaatcaat aaattaaagg
     1921 gcacattaaa gtacacaata tttgtgccct tctctgtttc tttcccgtta tcagctagct
     1981 ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
     2041 tacttcgcgt ttgccacctg acgtctaaga aaaggaatat tcagcaattt gcccgtgccg
     2101 aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gctacgtaag aattcgcggc
     2161 cgcttctaga g
//