##gff-version 3
##sequence-region BBa_K300000 1 2171
BBa_K300000	.	terminator	2072	2149	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_K300000	.	stem_loop	2107	2128	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_K300000	.	sequence_feature	2099	2137	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_K300000	.	sequence_feature	195	600	.	+	0	ID=BBa_K300998;Name=BBa_K300998
BBa_K300000	.	sequence_feature	601	608	.	+	0	ID=BBa_G0001;Name=BBa_G0001
BBa_K300000	.	sequence_feature	1974	1979	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300000	.	sequence_feature	1974	1979	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300000	.	sequence_feature	2150	2171	.	+	0	ID=BBa_G00000;Name=BBa_G00000
BBa_K300000	.	sequence_feature	2156	2163	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
BBa_K300000	.	sequence_feature	2165	2170	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
BBa_K300000	.	engineered_region	2150	2171	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
BBa_K300000	.	sequence_feature	2150	2155	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
BBa_K300000	.	primer	2052	2071	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_K300000	.	primer_binding_site	2052	2071	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
BBa_K300000	.	engineered_region	746	1514	.	+	0	ID=BBa_K300999;Name=BBa_K300999
BBa_K300000	.	CDS	746	1408	.	+	0	ID=annotation2081291;Name=cmR(rev);Parent=BBa_K300999
BBa_K300000	.	sequence_feature	152	187	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_K300000	.	sequence_feature	1564	1973	.	+	0	ID=BBa_K300991;Name=BBa_K300991
BBa_K300000	.	sequence_feature	188	194	.	+	0	ID=BBa_K300994;Name=BBa_K300994
BBa_K300000	.	sequence_feature	1515	1521	.	+	0	ID=BBa_K300995;Name=BBa_K300995
BBa_K300000	.	sequence_feature	1522	1557	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_K300000	.	sequence_feature	1558	1563	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300000	.	sequence_feature	1558	1563	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300000	.	sequence_feature	91	110	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_K300000	.	primer_binding_site	91	110	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_K300000	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
BBa_K300000	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
BBa_K300000	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
BBa_K300000	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
BBa_K300000	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
BBa_K300000	.	terminator	609	737	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_K300000	.	engineered_region	658	737	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_K300000	.	engineered_region	609	649	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_K300000	.	terminator	22	90	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_K300000	.	stem_loop	32	63	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_K300000	.	sequence_feature	25	64	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_K300000	.	sequence_feature	738	745	.	+	0	ID=BBa_G0001;Name=BBa_G0001
BBa_K300000	.	terminator	1980	2051	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_K300000	.	stem_loop	1988	2022	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_K300000	.	terminator	111	151	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_K300000	.	stem_loop	124	143	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
>BBa_K300000
tactagtagcggccgctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttgggg
ttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagt
tcctatactttctagagaataggaacttccctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttc
acgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctc
tcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagca
acttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaac
ggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacagga
acacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccg
aaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactg
agcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattc
attaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcg
ccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactgg
tgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcacc
gtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaac
gtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggcca
tacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctt
tacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctca
aaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttag
ctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttac
gtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaat
cagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtaca
catttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcag
agtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgt
aatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattc
aaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagc
tagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgc
cacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtga
gttgattgctacgtaagaattcgcggccgcttctagag