BBa_K300994 1 scar SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - Reverse complement of <partinfo>BBa_K300996</partinfo>. false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300996</partinfo> false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni BBa_K300999 1 CmR chloramphenicol resistance cassette (rev) 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z <partinfo>BBa_P1004</partinfo> Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator). false false _420_ 0 2621 9 Not in stock false see <partinfo>BBa_P1004</partinfo> for detailed information. false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni annotation2081291 1 cmR(rev) range2081291 1 1 663 BBa_B0062 1 BBa_B0062 Terminator (Reverse B0052) 2004-01-29T12:00:00Z 2015-08-31T04:07:21Z Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon. false false _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318637 1 stem_loop range318637 1 14 33 BBa_J72001 1 FRT {FRT} recombination site for flp recombinase in BBb 2008-07-14T11:00:00Z 2015-05-08T01:08:26Z Later Later false false _171_ 0 483 171 Not in stock false N/A false John Anderson BBa_B0054 1 BBa_B0054 Transcriptional terminator 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1747359 1 stem loop range1747359 1 11 42 annotation1783342 1 identical to E. coli MG1655 between tonB and yciA range1783342 1 4 43 BBa_K300991 1 phi80 attP phi80 phage attP integration site 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end. false false _420_ 0 2621 9 Not in stock true See <partinfo>BBa_J72004</partinfo> for more information. false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni BBa_B0053 1 BBa_B0053 Terminator (His) 2004-01-29T12:00:00Z 2015-08-31T04:07:20Z Terminator from the E.coli His operon false true _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318602 1 stem_loop range318602 1 9 43 BBa_G00102 1 VR Reverse BioBrick primer annealing site (VR binding site) 2006-02-03T12:00:00Z 2015-08-31T04:07:28Z This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1961229 1 VR range1961229 1 1 20 BBa_B0055 1 BBa_B0055 -- No description -- 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed upstream of multiple cloning site in new pSB series plasmids. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783311 1 identical to bacteriophage T3 range1783311 1 28 66 annotation1783301 1 stem loop from bacteriophage T3 range1783301 1 36 57 BBa_B0045 1 NheI NheI restriction enzyme site (XbaI, SpeI compatible overhang) 2006-03-13T12:00:00Z 2015-08-31T04:07:20Z NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1822724 1 NheI site range1822724 1 1 6 BBa_G0001 1 scar XbaI/SpeI mixed site, reverse 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI XbaI/SpeI mixed site, reverse false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0025 1 BBa_B0025 double terminator (B0015), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false true _1_ 0 24 7 In stock false true Caitlin Conboy annotation369702 1 B0012 range369702 1 1 41 annotation369703 1 B0010 range369703 1 50 129 BBa_G00000 1 Prefix BioBrick cloning site prefix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks prefix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1934224 1 XbaI site range1934224 1 16 21 annotation1961228 1 NotI site range1961228 1 7 14 annotation1934225 1 Prefix range1934225 1 1 22 annotation1934223 1 EcoRI site range1934223 1 1 6 BBa_K300998 1 R6K ori [R6K] Origin of replication (rev) 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z See <partinfo>BBa_J61001</partinfo> for detailed information. Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin). false false _420_ 0 2621 9 Not in stock false <partinfo>BBa_J61001</partinfo> false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni BBa_K300000 1 BBa_K300000 BioBrick integrative base vector for E. coli 2010-10-02T11:00:00Z 2015-05-08T01:11:51Z - - false false _420_ 0 2621 9 Not in stock true - false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi component2081557 1 BBa_K300991 component2081544 1 BBa_K300994 component2081563 1 BBa_G00100 component2081553 1 BBa_K300995 component2081535 1 BBa_G00001 component2081571 1 BBa_G00000 component2081556 1 BBa_B0045 component2081554 1 BBa_J72001 component2081546 1 BBa_G0001 component2081542 1 BBa_B0062 component2081545 1 BBa_K300998 component2081540 1 BBa_G00102 component2081543 1 BBa_J72001 component2081552 1 BBa_K300999 component2081566 1 BBa_B0055 component2081561 1 BBa_B0053 component2081559 1 BBa_B0045 component2081550 1 BBa_G0001 component2081549 1 BBa_B0025 component2081538 1 BBa_B0054 annotation2081566 1 BBa_B0055 range2081566 1 2072 2149 annotation2081546 1 BBa_G0001 range2081546 1 601 608 annotation2081549 1 BBa_B0025 range2081549 1 609 737 annotation2081559 1 BBa_B0045 range2081559 1 1974 1979 annotation2081545 1 BBa_K300998 range2081545 1 195 600 annotation2081571 1 BBa_G00000 range2081571 1 2150 2171 annotation2081563 1 BBa_G00100 range2081563 1 2052 2071 annotation2081543 1 BBa_J72001 range2081543 1 152 187 annotation2081557 1 BBa_K300991 range2081557 1 1564 1973 annotation2081542 1 BBa_B0062 range2081542 1 111 151 annotation2081556 1 BBa_B0045 range2081556 1 1558 1563 annotation2081544 1 BBa_K300994 range2081544 1 188 194 annotation2081554 1 BBa_J72001 range2081554 1 1522 1557 annotation2081553 1 BBa_K300995 range2081553 1 1515 1521 annotation2081552 1 BBa_K300999 range2081552 1 746 1514 annotation2081535 1 BBa_G00001 range2081535 1 1 21 annotation2081540 1 BBa_G00102 range2081540 1 91 110 annotation2081538 1 BBa_B0054 range2081538 1 22 90 annotation2081561 1 BBa_B0053 range2081561 1 1980 2051 annotation2081550 1 BBa_G0001 range2081550 1 738 745 BBa_G00100 1 VF2 Forward primer for sequencing/amplifying BioBrick parts (VF2) 2004-05-24T11:00:00Z 2015-08-31T04:07:28Z Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors. false false _11_1_ 0 60 7 Not in stock false false Tom Knight annotation1934505 1 VF2 range1934505 1 1 20 BBa_K300995 1 scar AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - Reverse complement of <partinfo>BBa_K300997</partinfo> false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300997</partinfo> false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni BBa_G00001 1 Suffix BioBrick cloning site suffix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks suffix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1821544 1 Suffix range1821544 1 1 21 annotation1934226 1 SpeI range1934226 1 2 7 annotation1934228 1 NotI range1934228 1 9 16 annotation1934227 1 PstI range1934227 1 16 21 BBa_B0045_sequence 1 gctagc BBa_K300995_sequence 1 ctctagg BBa_J72001_sequence 1 gcgaagttcctatactttctagagaataggaacttc BBa_K300998_sequence 1 gcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatca BBa_B0062_sequence 1 cagataaaaaaaatccttagctttcgctaaggatgatttct BBa_B0025_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg BBa_B0055_sequence 1 aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa BBa_B0054_sequence 1 attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_G00000_sequence 1 gaattcgcggccgcttctagag BBa_K300991_sequence 1 catgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatca BBa_G00001_sequence 1 tactagtagcggccgctgcag BBa_K300999_sequence 1 ttattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac BBa_B0053_sequence 1 tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt BBa_K300000_sequence 1 tactagtagcggccgctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatactttctagagaataggaacttccctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattcgcggccgcttctagag BBa_G00100_sequence 1 tgccacctgacgtctaagaa BBa_G0001_sequence 1 ctctagta BBa_K300994_sequence 1 cctagta BBa_G00102_sequence 1 gctcactcaaaggcggtaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z