BioBrick integrative base vector for E. coli
Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
This part is the standard BioBricks prefix.
Forward primer for sequencing/amplifying BioBrick parts (VF2)
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
{FRT} recombination site for flp recombinase in BBb
chloramphenicol resistance cassette (rev)
Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator).
see <partinfo>BBa_P1004</partinfo> for detailed information.
Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
See <partinfo>BBa_J61001</partinfo> for detailed information.
Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin).
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev
Reverse complement of <partinfo>BBa_K300997</partinfo>
Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev
Reverse complement of <partinfo>BBa_K300996</partinfo>.
This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end.
See <partinfo>BBa_J72004</partinfo> for more information.
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
This part is the standard BioBricks suffix.
Reverse BioBrick primer annealing site (VR binding site)
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
Conversion of the iGEM parts registry to SBOL2.1