BBa_K300989 1 KanMX(lox) [LoxP-KanMX-LoxP] excisable marker for yeast 2010-10-02T11:00:00Z 2015-05-08T01:11:52Z pUG6 vector (GenBank: AF298793.1). This cassette is to be used in yeast. It is composed by the kanMX gene from the E. coli transposon Tn903 that confers the resistance to the aminoglycoside antibiotic G418 to yeast (commonly used at 200 ug/ml working concentration). This gene is expressed by the TEF2 promoter/RBS and terminated by the TEF2 transcriptional terminator from Ashbya gossypii filamentous fungus. The cassette is flanked by two loxP sites and for this reason it can be excised with Cre/loxP mediated marker removal recombination procedure. ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to select positive integrants in the yeast genome and then it can be eliminated through Cre/loxP procedure. This enables the engineering of yeast genome in multiple steps, always using the same dominant selection marker. false false _420_ 0 2621 9 Not in stock true The original sequence of loxP-KanMX-loxP from pUG6 vector has two NsiI restriction sites in kanR(Tn903) coding sequence and they have been removed in this part by changing codons. The TEF2 promoter has a XbaI and a PstI restriction sites, which have not been removed to avoid the change of the promoter strength. For this reason, this part is not fully compatible with RFC10 BioBrick Standard Assembly, but it can be assembled by using EcoRI-SpeI enzymes. false Lorenzo Pasotti, Giacomo Zambianchi, Federica Sampietro, Paolo Magni annotation2081364 1 T->C range2081364 1 975 975 annotation2081366 1 BBa_J61046 (loxP) range2081366 1 1508 1541 annotation2081361 1 TEF2 (A. gossypii)+RBS range2081361 1 35 432 annotation2081362 1 kanR(Tn903) range2081362 1 433 1242 annotation2081367 1 kanMX cassette range2081367 1 35 1507 annotation2081365 1 TEF2 terminator (A. gossypii) range2081365 1 1243 1507 annotation2081363 1 A->T range2081363 1 708 708 annotation2081360 1 BBa_J61046 (loxP) range2081360 1 1 34 BBa_G00000 1 Prefix BioBrick cloning site prefix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks prefix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1934225 1 Prefix range1934225 1 1 22 annotation1934223 1 EcoRI site range1934223 1 1 6 annotation1934224 1 XbaI site range1934224 1 16 21 annotation1961228 1 NotI site range1961228 1 7 14 BBa_K300990 1 SbfI SbfI restriction site 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - This site can also be cut with PstI restriction enzyme. false false _420_ 0 2621 9 Not in stock false Restriction site sequence false Lorenzo Pasotti, Paolo Magni BBa_B0048 1 AvrII AvrII restriction enzyme site (XbaI, SpeI compatible overhang) 2006-03-13T12:00:00Z 2015-08-31T04:07:20Z AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1822744 1 AvrII site range1822744 1 1 6 BBa_K300988 1 pSB1A2 pSB1A2 without Prefix and Suffix 2010-10-02T11:00:00Z 2015-05-08T01:11:52Z See <partinfo>pSB1A2</partinfo>. This part is identical to pSB1A2, but it does not have the Prefix and Suffix. It can be useful to specify the sequence of new vectors constructed with BioBrick parts, such as <partinfo>BBa_K300001</partinfo>. false false _420_ 0 2621 9 Not in stock false No changes have been made to <partinfo>pSB1A2</partinfo> sequence. false Susanna Zucca, Federica Sampietro, Paolo Magni annotation2081411 1 rep (pMB1) (rev) range2081411 1 162 776 annotation2081410 1 VR range2081410 1 58 77 annotation2081413 1 APr (rev) range2081413 1 936 1796 annotation2081412 1 ORI range2081412 1 177 177 annotation2081416 1 terminator range2081416 1 2008 2027 annotation2081415 1 VF2 range2081415 1 1919 1938 annotation2081414 1 pBLA (rev) range2081414 1 1831 1831 BBa_K300987 1 HR2-GAL10 Homologous region in S288C yeast genome (GAL10 ORF) 2010-10-02T11:00:00Z 2015-05-08T01:11:52Z - - false false _420_ 0 2621 9 Not in stock true - false Susanna Zucca, Federica Sampietro, Paolo Magni BBa_K300993 1 scar EcoRI/MfeI mixed site 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - EcoRI (<partinfo>BBa_M31983</partinfo>) and MfeI (<partinfo>BBa_B0047</partinfo>) have a compatible overhang (AATT). The EcoRI/MfeI mixed site is GAATTG. false false _420_ 0 2621 9 Not in stock false Mixed site false Susanna Zucca, Federica Sampietro, Paolo Magni BBa_B0045 1 NheI NheI restriction enzyme site (XbaI, SpeI compatible overhang) 2006-03-13T12:00:00Z 2015-08-31T04:07:20Z NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1822724 1 NheI site range1822724 1 1 6 BBa_K300992 1 scar NsiI/PstI mixed site 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - NsiI (<partinfo>BBa_B0046</partinfo>) and PstI have a compatible overhang (TGCA). The NsiI/PstI mixed site is ATGCAG. false false _420_ 0 2621 9 Not in stock false Mixed site false Susanna Zucca, Federica Sampietro, Paolo Magni BBa_M31992 1 SpeI SpeI restriction enzyme site 2007-02-28T12:00:00Z 2015-05-08T01:14:01Z none SpeI restriction enzyme site false false _102_ 0 1373 102 Not in stock false none false Tiffany Guo BBa_K300986 1 HR1-GAL1 Homologous region in S288C yeast genome (GAL1 ORF) 2010-10-02T11:00:00Z 2015-05-08T01:11:52Z - - false false _420_ 0 2621 9 Not in stock true - false Susanna Zucca, Federica Sampietro, Paolo Magni BBa_G0012 1 T The nucleotide T 2007-07-22T11:00:00Z 2015-08-31T04:07:28Z None. This is a part consisting of only a single nucleotide ... T. It can act as a spacer between restriction sites and parts. false false _41_ 0 126 162 Not in stock false None. false Reshma Shetty BBa_K300001 1 BBa_K300001 BioBrick integrative base vector for S. cerevisiae 2010-10-02T11:00:00Z 2015-05-08T01:11:51Z - - false false _420_ 0 2621 9 Not in stock true - false Susanna Zucca, Lorenzo Pasotti, Federica Sampietro, Paolo Magni component2081774 1 BBa_B0045 component2081775 1 BBa_K300987 component2081762 1 BBa_G0012 component2081799 1 BBa_G00000 component2081778 1 BBa_K300990 component2081777 1 BBa_B0045 component2081763 1 BBa_M31992 component2081792 1 BBa_K300986 component2081779 1 BBa_K300992 component2081789 1 BBa_K300990 component2081794 1 BBa_B0048 component2081787 1 BBa_K300988 component2081791 1 BBa_B0048 component2081788 1 BBa_K300993 component2081772 1 BBa_K300989 annotation2081777 1 BBa_B0045 range2081777 1 1600 1605 annotation2081789 1 BBa_K300990 range2081789 1 3662 3669 annotation2081778 1 BBa_K300990 range2081778 1 1606 1613 annotation2081775 1 BBa_K300987 range2081775 1 1555 1599 annotation2081787 1 BBa_K300988 range2081787 1 1620 3655 annotation2081774 1 BBa_B0045 range2081774 1 1549 1554 annotation2081799 1 BBa_G00000 range2081799 1 3727 3748 annotation2081763 1 BBa_M31992 range2081763 1 2 7 annotation2081791 1 BBa_B0048 range2081791 1 3670 3675 annotation2081788 1 BBa_K300993 range2081788 1 3656 3661 annotation2081792 1 BBa_K300986 range2081792 1 3676 3720 annotation2081762 1 BBa_G0012 range2081762 1 1 1 annotation2081772 1 BBa_K300989 range2081772 1 8 1548 annotation2081794 1 BBa_B0048 range2081794 1 3721 3726 annotation2081779 1 BBa_K300992 range2081779 1 1614 1619 BBa_B0045_sequence 1 gctagc BBa_K300986_sequence 1 gtgtgaaccaatgtatccagcaccacctgtaaccaaaacaatttt BBa_K300992_sequence 1 atgcag BBa_K300993_sequence 1 gaattg BBa_K300990_sequence 1 cctgcagg BBa_G0012_sequence 1 t BBa_B0048_sequence 1 cctagg BBa_K300989_sequence 1 ataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttat BBa_K300988_sequence 1 gcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctg BBa_G00000_sequence 1 gaattcgcggccgcttctagag BBa_K300001_sequence 1 tactagtataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttatgctagccctgagttcaattctagcgcaaaggaattaccaagaccattggccgctagccctgcaggatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattgcctgcaggcctagggtgtgaaccaatgtatccagcaccacctgtaaccaaaacaattttcctagggaattcgcggccgcttctagag BBa_M31992_sequence 1 actagt BBa_K300987_sequence 1 cctgagttcaattctagcgcaaaggaattaccaagaccattggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z