##gff-version 3
##sequence-region BBa_K300001 1 3748
BBa_K300001	.	sequence_feature	1555	1599	.	+	0	ID=BBa_K300987;Name=BBa_K300987
BBa_K300001	.	sequence_feature	1549	1554	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300001	.	sequence_feature	1549	1554	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300001	.	sequence_feature	3656	3661	.	+	0	ID=BBa_K300993;Name=BBa_K300993
BBa_K300001	.	sequence_feature	3676	3720	.	+	0	ID=BBa_K300986;Name=BBa_K300986
BBa_K300001	.	engineered_region	8	1548	.	+	0	ID=BBa_K300989;Name=BBa_K300989
BBa_K300001	.	engineered_region	8	41	.	+	0	ID=annotation2081360;Name=BBa_J61046 (loxP);Parent=BBa_K300989
BBa_K300001	.	CDS	440	1249	.	+	0	ID=annotation2081362;Name=kanR(Tn903);Parent=BBa_K300989
BBa_K300001	.	point_mutation	982	982	.	+	0	ID=annotation2081364;Name=T->C;Parent=BBa_K300989
BBa_K300001	.	sequence_feature	1250	1514	.	+	0	ID=annotation2081365;Name=TEF2 terminator (A. gossypii);Parent=BBa_K300989
BBa_K300001	.	engineered_region	1515	1548	.	+	0	ID=annotation2081366;Name=BBa_J61046 (loxP);Parent=BBa_K300989
BBa_K300001	.	point_mutation	715	715	.	+	0	ID=annotation2081363;Name=A->T;Parent=BBa_K300989
BBa_K300001	.	sequence_feature	42	1514	.	+	0	ID=annotation2081367;Name=kanMX cassette;Parent=BBa_K300989
BBa_K300001	.	promoter	42	439	.	+	0	ID=annotation2081361;Name=TEF2 (A. gossypii)+RBS;Parent=BBa_K300989
BBa_K300001	.	sequence_feature	1614	1619	.	+	0	ID=BBa_K300992;Name=BBa_K300992
BBa_K300001	.	sequence_feature	1600	1605	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300001	.	sequence_feature	1600	1605	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300001	.	sequence_feature	3727	3748	.	+	0	ID=BBa_G00000;Name=BBa_G00000
BBa_K300001	.	sequence_feature	3733	3740	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
BBa_K300001	.	engineered_region	3727	3748	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
BBa_K300001	.	sequence_feature	3727	3732	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
BBa_K300001	.	sequence_feature	3742	3747	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
BBa_K300001	.	plasmid_vector	1620	3655	.	+	0	ID=BBa_K300988;Name=BBa_K300988
BBa_K300001	.	CDS	1781	2395	.	+	0	ID=annotation2081411;Name=rep (pMB1) (rev);Parent=BBa_K300988
BBa_K300001	.	CDS	2555	3415	.	+	0	ID=annotation2081413;Name=APr (rev);Parent=BBa_K300988
BBa_K300001	.	primer_binding_site	3538	3557	.	+	0	ID=annotation2081415;Name=VF2;Parent=BBa_K300988
BBa_K300001	.	sequence_feature	3450	3450	.	+	0	ID=annotation2081414;Name=pBLA (rev);Parent=BBa_K300988
BBa_K300001	.	stem_loop	3627	3646	.	+	0	ID=annotation2081416;Name=terminator;Parent=BBa_K300988
BBa_K300001	.	sequence_feature	1796	1796	.	+	0	ID=annotation2081412;Name=ORI;Parent=BBa_K300988
BBa_K300001	.	primer_binding_site	1677	1696	.	+	0	ID=annotation2081410;Name=VR;Parent=BBa_K300988
BBa_K300001	.	sequence_feature	1606	1613	.	+	0	ID=BBa_K300990;Name=BBa_K300990
BBa_K300001	.	sequence_feature	3670	3675	.	+	0	ID=BBa_B0048;Name=BBa_B0048
BBa_K300001	.	sequence_feature	3670	3675	.	+	0	ID=annotation1822744;Name=AvrII site;Parent=BBa_B0048
BBa_K300001	.	sequence_feature	2	7	.	+	0	ID=BBa_M31992;Name=BBa_M31992
BBa_K300001	.	sequence_feature	3721	3726	.	+	0	ID=BBa_B0048;Name=BBa_B0048
BBa_K300001	.	sequence_feature	3721	3726	.	+	0	ID=annotation1822744;Name=AvrII site;Parent=BBa_B0048
BBa_K300001	.	sequence_feature	1	1	.	+	0	ID=BBa_G0012;Name=BBa_G0012
BBa_K300001	.	sequence_feature	3662	3669	.	+	0	ID=BBa_K300990;Name=BBa_K300990
>BBa_K300001
tactagtataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgcc
gggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgt
gactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgc
gaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtcccca
cgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatc
ttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcg
attaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatc
tatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacag
atgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatga
tgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaat
attgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcg
tatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatgg
ctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgat
ttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagacc
gataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaata
tggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaata
aaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttag
cgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtc
aatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctc
gagaacccttaatataacttcgtataatgtatgctatacgaagttatgctagccctgagttcaattctagcgcaaagga
attaccaagaccattggccgctagccctgcaggatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggc
tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacat
gtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccct
gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcccc
ctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaag
cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcac
gaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttat
cgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtg
gcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt
ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaa
aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggat
tttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagt
atatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgtt
catccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaat
gataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagt
ggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaata
gtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccgg
ttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgtt
gtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccg
taagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttg
cccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgggg
cgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcat
cttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacg
gaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatac
atatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc
tttcgctaaggatgatttctggaattgcctgcaggcctagggtgtgaaccaatgtatccagcaccacctgtaaccaaaa
caattttcctagggaattcgcggccgcttctagag