BBa_K300001
type: ComponentDefinition
persistent identity: BBa K300001
display id: BBa_K300001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick integrative base vector for S. cerevisiae
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: -
star: false
creator: Susanna Zucca, Lorenzo Pasotti, Federica Sampietro, Paolo Magni
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_K300993
component: BBa_K300988
component: BBa_B0048
component: BBa_K300990
component: BBa_B0045
component: BBa_K300987
component: BBa_K300992
component: BBa_K300986
component: BBa_G00000
component: BBa_M31992
component: BBa_G0012
component: BBa_K300989
sequence annotation: BBa_G0012
sequence annotation: BBa_M31992
sequence annotation: BBa_K300989
sequence annotation: BBa_B0045
sequence annotation: BBa_K300987
sequence annotation: BBa_K300990
sequence annotation: BBa_K300992
sequence annotation: BBa_K300988
sequence annotation: BBa_K300993
sequence annotation: BBa_B0048
sequence annotation: BBa_K300986
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K300001_sequence/1

BBa_K300993
type: Component
persistent identity: component2081788
display id: component2081788
version: 1
top level: BBa_K300001
definition: scar
access: public

BBa_K300988
type: Component
persistent identity: component2081787
display id: component2081787
version: 1
top level: BBa_K300001
definition: pSB1A2
access: public

BBa_B0048
type: Component
persistent identity: component2081791
display id: component2081791
version: 1
top level: BBa_K300001
definition: AvrII
access: public

BBa_K300990
type: Component
persistent identity: component2081789
display id: component2081789
version: 1
top level: BBa_K300001
definition: SbfI
access: public

BBa_B0045
type: Component
persistent identity: component2081777
display id: component2081777
version: 1
top level: BBa_K300001
definition: NheI
access: public

BBa_K300987
type: Component
persistent identity: component2081775
display id: component2081775
version: 1
top level: BBa_K300001
definition: HR2-GAL10
access: public

BBa_K300992
type: Component
persistent identity: component2081779
display id: component2081779
version: 1
top level: BBa_K300001
definition: scar
access: public

BBa_K300990
type: Component
persistent identity: component2081778
display id: component2081778
version: 1
top level: BBa_K300001
definition: SbfI
access: public

BBa_B0048
type: Component
persistent identity: component2081794
display id: component2081794
version: 1
top level: BBa_K300001
definition: AvrII
access: public

BBa_K300986
type: Component
persistent identity: component2081792
display id: component2081792
version: 1
top level: BBa_K300001
definition: HR1-GAL1
access: public

BBa_G00000
type: Component
persistent identity: component2081799
display id: component2081799
version: 1
top level: BBa_K300001
definition: Prefix
access: public

BBa_M31992
type: Component
persistent identity: component2081763
display id: component2081763
version: 1
top level: BBa_K300001
definition: SpeI
access: public

BBa_G0012
type: Component
persistent identity: component2081762
display id: component2081762
version: 1
top level: BBa_K300001
definition: T
access: public

BBa_B0045
type: Component
persistent identity: component2081774
display id: component2081774
version: 1
top level: BBa_K300001
definition: NheI
access: public

BBa_K300989
type: Component
persistent identity: component2081772
display id: component2081772
version: 1
top level: BBa_K300001
definition: KanMX(lox)
access: public

BBa_G0012
type: SequenceAnnotation
persistent identity: annotation2081762
display id: annotation2081762
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081762/range2081762/1
component: BBa_G0012

https://synbiohub.org/public/igem/BBa_K300001/annotation2081762/range2081762/1
type: Range
persistent identity: range2081762
display id: range2081762
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1
end: 1
orientation: inline

BBa_M31992
type: SequenceAnnotation
persistent identity: annotation2081763
display id: annotation2081763
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081763/range2081763/1
component: BBa_M31992

https://synbiohub.org/public/igem/BBa_K300001/annotation2081763/range2081763/1
type: Range
persistent identity: range2081763
display id: range2081763
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 2
end: 7
orientation: inline

BBa_K300989
type: SequenceAnnotation
persistent identity: annotation2081772
display id: annotation2081772
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081772/range2081772/1
component: BBa_K300989

https://synbiohub.org/public/igem/BBa_K300001/annotation2081772/range2081772/1
type: Range
persistent identity: range2081772
display id: range2081772
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 8
end: 1548
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2081774
display id: annotation2081774
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081774/range2081774/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300001/annotation2081774/range2081774/1
type: Range
persistent identity: range2081774
display id: range2081774
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1549
end: 1554
orientation: inline

BBa_K300987
type: SequenceAnnotation
persistent identity: annotation2081775
display id: annotation2081775
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081775/range2081775/1
component: BBa_K300987

https://synbiohub.org/public/igem/BBa_K300001/annotation2081775/range2081775/1
type: Range
persistent identity: range2081775
display id: range2081775
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1555
end: 1599
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2081777
display id: annotation2081777
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081777/range2081777/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300001/annotation2081777/range2081777/1
type: Range
persistent identity: range2081777
display id: range2081777
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1600
end: 1605
orientation: inline

BBa_K300990
type: SequenceAnnotation
persistent identity: annotation2081778
display id: annotation2081778
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081778/range2081778/1
component: BBa_K300990

https://synbiohub.org/public/igem/BBa_K300001/annotation2081778/range2081778/1
type: Range
persistent identity: range2081778
display id: range2081778
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1606
end: 1613
orientation: inline

BBa_K300992
type: SequenceAnnotation
persistent identity: annotation2081779
display id: annotation2081779
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081779/range2081779/1
component: BBa_K300992

https://synbiohub.org/public/igem/BBa_K300001/annotation2081779/range2081779/1
type: Range
persistent identity: range2081779
display id: range2081779
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1614
end: 1619
orientation: inline

BBa_K300988
type: SequenceAnnotation
persistent identity: annotation2081787
display id: annotation2081787
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081787/range2081787/1
component: BBa_K300988

https://synbiohub.org/public/igem/BBa_K300001/annotation2081787/range2081787/1
type: Range
persistent identity: range2081787
display id: range2081787
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 1620
end: 3655
orientation: inline

BBa_K300993
type: SequenceAnnotation
persistent identity: annotation2081788
display id: annotation2081788
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081788/range2081788/1
component: BBa_K300993

https://synbiohub.org/public/igem/BBa_K300001/annotation2081788/range2081788/1
type: Range
persistent identity: range2081788
display id: range2081788
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 3656
end: 3661
orientation: inline

BBa_K300990
type: SequenceAnnotation
persistent identity: annotation2081789
display id: annotation2081789
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081789/range2081789/1
component: BBa_K300990

https://synbiohub.org/public/igem/BBa_K300001/annotation2081789/range2081789/1
type: Range
persistent identity: range2081789
display id: range2081789
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 3662
end: 3669
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2081791
display id: annotation2081791
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081791/range2081791/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300001/annotation2081791/range2081791/1
type: Range
persistent identity: range2081791
display id: range2081791
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 3670
end: 3675
orientation: inline

BBa_K300986
type: SequenceAnnotation
persistent identity: annotation2081792
display id: annotation2081792
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081792/range2081792/1
component: BBa_K300986

https://synbiohub.org/public/igem/BBa_K300001/annotation2081792/range2081792/1
type: Range
persistent identity: range2081792
display id: range2081792
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 3676
end: 3720
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2081794
display id: annotation2081794
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081794/range2081794/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300001/annotation2081794/range2081794/1
type: Range
persistent identity: range2081794
display id: range2081794
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 3721
end: 3726
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2081799
display id: annotation2081799
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081799/range2081799/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K300001/annotation2081799/range2081799/1
type: Range
persistent identity: range2081799
display id: range2081799
version: 1
top level: BBa_K300001
direction: direction/reverse
start: 3727
end: 3748
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

KanMX(lox)
type: ComponentDefinition
persistent identity: BBa K300989
display id: BBa_K300989
version: 1
was generated by: iGEM to SBOL conversion
description: [LoxP-KanMX-LoxP] excisable marker for yeast
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: pUG6 vector (GenBank: AF298793.1).
owned by: james
owned by: myers
mutable description: This cassette is to be used in yeast. It is composed by the kanMX gene from the E. coli transposon Tn903 that confers the resistance to the aminoglycoside antibiotic G418 to yeast (commonly used at 200 ug/ml working concentration). This gene is expressed by the TEF2 promoter/RBS and terminated by the TEF2 transcriptional terminator from Ashbya gossypii filamentous fungus. The cassette is flanked by two loxP sites and for this reason it can be excised with Cre/loxP mediated marker removal recombination procedure. ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to select positive integrants in the yeast genome and then it can be eliminated through Cre/loxP procedure. This enables the engineering of yeast genome in multiple steps, always using the same dominant selection marker.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The original sequence of loxP-KanMX-loxP from pUG6 vector has two NsiI restriction sites in kanR(Tn903) coding sequence and they have been removed in this part by changing codons. The TEF2 promoter has a XbaI and a PstI restriction sites, which have not been removed to avoid the change of the promoter strength. For this reason, this part is not fully compatible with RFC10 BioBrick Standard Assembly, but it can be assembled by using EcoRI-SpeI enzymes.
star: false
creator: Lorenzo Pasotti, Giacomo Zambianchi, Federica Sampietro, Paolo Magni
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: BBa_J61046 (loxP)
sequence annotation: TEF2 (A. gossypii)+RBS
sequence annotation: kanMX cassette
sequence annotation: kanR(Tn903)
sequence annotation: A->T
sequence annotation: T->C
sequence annotation: TEF2 terminator (A. gossypii)
sequence: https://synbiohub.org/public/igem/BBa_K300989_sequence/1

BBa_J61046 (loxP)
type: SequenceAnnotation
persistent identity: annotation2081360
display id: annotation2081360
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081360/range2081360/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K300989/annotation2081360/range2081360/1
type: Range
persistent identity: range2081360
display id: range2081360
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 1
end: 34
orientation: inline

TEF2 (A. gossypii)+RBS
type: SequenceAnnotation
persistent identity: annotation2081361
display id: annotation2081361
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081361/range2081361/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K300989/annotation2081361/range2081361/1
type: Range
persistent identity: range2081361
display id: range2081361
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 35
end: 432
orientation: inline

kanMX cassette
type: SequenceAnnotation
persistent identity: annotation2081367
display id: annotation2081367
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081367/range2081367/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K300989/annotation2081367/range2081367/1
type: Range
persistent identity: range2081367
display id: range2081367
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 35
end: 1507
orientation: inline

kanR(Tn903)
type: SequenceAnnotation
persistent identity: annotation2081362
display id: annotation2081362
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081362/range2081362/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K300989/annotation2081362/range2081362/1
type: Range
persistent identity: range2081362
display id: range2081362
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 433
end: 1242
orientation: inline

A->T
type: SequenceAnnotation
persistent identity: annotation2081363
display id: annotation2081363
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081363/range2081363/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K300989/annotation2081363/range2081363/1
type: Range
persistent identity: range2081363
display id: range2081363
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 708
end: 708
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation2081364
display id: annotation2081364
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081364/range2081364/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K300989/annotation2081364/range2081364/1
type: Range
persistent identity: range2081364
display id: range2081364
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 975
end: 975
orientation: inline

TEF2 terminator (A. gossypii)
type: SequenceAnnotation
persistent identity: annotation2081365
display id: annotation2081365
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081365/range2081365/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K300989/annotation2081365/range2081365/1
type: Range
persistent identity: range2081365
display id: range2081365
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 1243
end: 1507
orientation: inline

BBa_J61046 (loxP)
type: SequenceAnnotation
persistent identity: annotation2081366
display id: annotation2081366
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081366/range2081366/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K300989/annotation2081366/range2081366/1
type: Range
persistent identity: range2081366
display id: range2081366
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 1508
end: 1541
orientation: inline

T
type: ComponentDefinition
persistent identity: BBa G0012
display id: BBa_G0012
version: 1
was generated by: iGEM to SBOL conversion
description: The nucleotide T
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: None.
owned by: james
owned by: myers
mutable description: This is a part consisting of only a single nucleotide ... T. It can act as a spacer between restriction sites and parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0012_sequence/1

SpeI
type: ComponentDefinition
persistent identity: BBa M31992
display id: BBa_M31992
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: SpeI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31992_sequence/1

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa K300993
display id: BBa_K300993
version: 1
was generated by: iGEM to SBOL conversion
description: EcoRI/MfeI mixed site
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: EcoRI (<partinfo>BBa_M31983</partinfo>) and MfeI (<partinfo>BBa_B0047</partinfo>) have a compatible overhang (AATT). The EcoRI/MfeI mixed site is GAATTG.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Mixed site
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300993_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa K300992
display id: BBa_K300992
version: 1
was generated by: iGEM to SBOL conversion
description: NsiI/PstI mixed site
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: NsiI (<partinfo>BBa_B0046</partinfo>) and PstI have a compatible overhang (TGCA). The NsiI/PstI mixed site is ATGCAG.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Mixed site
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300992_sequence/1

SbfI
type: ComponentDefinition
persistent identity: BBa K300990
display id: BBa_K300990
version: 1
was generated by: iGEM to SBOL conversion
description: SbfI restriction site
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This site can also be cut with PstI restriction enzyme.
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Restriction site sequence
star: false
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300990_sequence/1

pSB1A2
type: ComponentDefinition
persistent identity: BBa K300988
display id: BBa_K300988
version: 1
was generated by: iGEM to SBOL conversion
description: pSB1A2 without Prefix and Suffix
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: See <partinfo>pSB1A2</partinfo>.
owned by: james
owned by: myers
mutable description: This part is identical to pSB1A2, but it does not have the Prefix and Suffix. It can be useful to specify the sequence of new vectors constructed with BioBrick parts, such as <partinfo>BBa_K300001</partinfo>.
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No changes have been made to <partinfo>pSB1A2</partinfo> sequence.
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
sequence annotation: VR
sequence annotation: rep (pMB1) (rev)
sequence annotation: ORI
sequence annotation: APr (rev)
sequence annotation: pBLA (rev)
sequence annotation: VF2
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K300988_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2081410
display id: annotation2081410
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081410/range2081410/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K300988/annotation2081410/range2081410/1
type: Range
persistent identity: range2081410
display id: range2081410
version: 1
top level: pSB1A2
direction: direction/reverse
start: 58
end: 77
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2081411
display id: annotation2081411
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081411/range2081411/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K300988/annotation2081411/range2081411/1
type: Range
persistent identity: range2081411
display id: range2081411
version: 1
top level: pSB1A2
direction: direction/reverse
start: 162
end: 776
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2081412
display id: annotation2081412
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081412/range2081412/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K300988/annotation2081412/range2081412/1
type: Range
persistent identity: range2081412
display id: range2081412
version: 1
top level: pSB1A2
direction: direction/forward
start: 177
end: 177
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation2081413
display id: annotation2081413
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081413/range2081413/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K300988/annotation2081413/range2081413/1
type: Range
persistent identity: range2081413
display id: range2081413
version: 1
top level: pSB1A2
direction: direction/reverse
start: 936
end: 1796
orientation: inline

pBLA (rev)
type: SequenceAnnotation
persistent identity: annotation2081414
display id: annotation2081414
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081414/range2081414/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K300988/annotation2081414/range2081414/1
type: Range
persistent identity: range2081414
display id: range2081414
version: 1
top level: pSB1A2
direction: direction/reverse
start: 1831
end: 1831
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2081415
display id: annotation2081415
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081415/range2081415/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K300988/annotation2081415/range2081415/1
type: Range
persistent identity: range2081415
display id: range2081415
version: 1
top level: pSB1A2
direction: direction/forward
start: 1919
end: 1938
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2081416
display id: annotation2081416
version: 1
top level: pSB1A2
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081416/range2081416/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K300988/annotation2081416/range2081416/1
type: Range
persistent identity: range2081416
display id: range2081416
version: 1
top level: pSB1A2
direction: direction/forward
start: 2008
end: 2027
orientation: inline

HR2-GAL10
type: ComponentDefinition
persistent identity: BBa K300987
display id: BBa_K300987
version: 1
was generated by: iGEM to SBOL conversion
description: Homologous region in S288C yeast genome (GAL10 ORF)
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: -
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300987_sequence/1

HR1-GAL1
type: ComponentDefinition
persistent identity: BBa K300986
display id: BBa_K300986
version: 1
was generated by: iGEM to SBOL conversion
description: Homologous region in S288C yeast genome (GAL1 ORF)
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: -
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300986_sequence/1

AvrII
type: ComponentDefinition
persistent identity: BBa B0048
display id: BBa_B0048
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_B0048_sequence/1

AvrII site
type: SequenceAnnotation
persistent identity: annotation1822744
display id: annotation1822744
version: 1
top level: AvrII
location: https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
type: Range
persistent identity: range1822744
display id: range1822744
version: 1
top level: AvrII
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_K300001_sequence/1
type: Sequence
persistent identity: BBa K300001 sequence
display id: BBa_K300001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttatgctagccctgagttcaattctagcgcaaaggaattaccaagaccattggccgctagccctgcaggatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300993_sequence/1
type: Sequence
persistent identity: BBa K300993 sequence
display id: BBa_K300993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300989_sequence/1
type: Sequence
persistent identity: BBa K300989 sequence
display id: BBa_K300989_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0012_sequence/1
type: Sequence
persistent identity: BBa G0012 sequence
display id: BBa_G0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: t
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31992_sequence/1
type: Sequence
persistent identity: BBa M31992 sequence
display id: BBa_M31992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300992_sequence/1
type: Sequence
persistent identity: BBa K300992 sequence
display id: BBa_K300992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300990_sequence/1
type: Sequence
persistent identity: BBa K300990 sequence
display id: BBa_K300990_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgcagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300988_sequence/1
type: Sequence
persistent identity: BBa K300988 sequence
display id: BBa_K300988_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300987_sequence/1
type: Sequence
persistent identity: BBa K300987 sequence
display id: BBa_K300987_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgagttcaattctagcgcaaaggaattaccaagaccattggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300986_sequence/1
type: Sequence
persistent identity: BBa K300986 sequence
display id: BBa_K300986_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgtgaaccaatgtatccagcaccacctgtaaccaaaacaatttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0048_sequence/1
type: Sequence
persistent identity: BBa B0048 sequence
display id: BBa_B0048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z