LOCUS       BBa_K300010             1870 bp    DNA     linear       14-Jun-2024
DEFINITION  PoPS-based self-inducible device
ACCESSION   BBa_K300010
VERSION     BBa_K300010.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    601..632
                     /label=LVA
     CDS             22..600
                     /label=luxI
     misc_feature    22..639
                     /label=BBa_C0061
     misc_feature    640..664
                     /label=Help:Barcodes
     CDS             22..639
                     /label=BBa_C0061
     misc_feature    1..664
                     /label=BBa_K081008
     stem_loop       684..727
                     /label=stem_loop
     misc_feature    673..752
                     /label=BBa_B0010
     terminator      673..752
                     /label=BBa_B0010
     stem_loop       768..787
                     /label=T7 TE
     misc_feature    794..794
                     /label=stop
     polyA_site      788..801
                     /label=polya
     misc_feature    761..801
                     /label=BBa_B0012
     terminator      761..801
                     /label=BBa_B0012
     terminator      673..801
                     /label=BBa_B0015
     misc_feature    1..801
                     /label=BBa_K300009
     misc_feature    810..828
                     /label=TetR 1
     misc_feature    810..863
                     /label=BBa_R0040
     promoter        829..834
                     /label=-35
     misc_feature    835..853
                     /label=TetR 2
     promoter        852..857
                     /label=-10
     promoter        810..863
                     /label=BBa_R0040
     misc_feature    876..879
                     /label=conserved
     RBS             872..883
                     /label=BBa_B0034
     misc_feature    890..891
                     /label=prefix
     misc_feature    1063..1063
                     /label=T
     misc_feature    1381..1381
                     /label=A
     CDS             890..1639
                     /label=luxR
     misc_feature    890..1645
                     /label=BBa_C0062
     misc_feature    1646..1670
                     /label=Help:Barcodes
     CDS             890..1645
                     /label=BBa_C0062
     stem_loop       1690..1733
                     /label=stem_loop
     misc_feature    1679..1758
                     /label=BBa_B0010
     terminator      1679..1758
                     /label=BBa_B0010
     stem_loop       1774..1793
                     /label=T7 TE
     misc_feature    1800..1800
                     /label=stop
     polyA_site      1794..1807
                     /label=polya
     misc_feature    1767..1807
                     /label=BBa_B0012
     terminator      1767..1807
                     /label=BBa_B0012
     terminator      1679..1807
                     /label=BBa_B0015
     misc_feature    1816..1835
                     /label=LuxR/HSL
     promoter        1835..1840
                     /label=-35
     promoter        1857..1862
                     /label=-10
     misc_feature    1868..1868
                     /label=start
     misc_feature    1816..1870
                     /label=BBa_R0062
     promoter        1816..1870
                     /label=BBa_R0062
     misc_feature    810..1870
                     /label=BBa_F2620
ORIGIN
        1 attaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
       61 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      121 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      181 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      241 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      301 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      361 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      421 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      481 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      541 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      601 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      661 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      721 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      781 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta
      841 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tgaaaaacat
      901 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga
      961 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc
     1021 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa
     1081 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta
     1141 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa
     1201 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt
     1261 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga
     1321 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc
     1381 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac
     1441 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc
     1501 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa
     1561 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga
     1621 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc
     1681 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1741 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1801 gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata
     1861 gtcgaataaa
//