##gff-version 3
##sequence-region BBa_K300010 1 1870
BBa_K300010	.	engineered_region	1	801	.	+	0	ID=BBa_K300009;Name=BBa_K300009
BBa_K300010	.	terminator	673	801	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K300009
BBa_K300010	.	terminator	673	752	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300010	.	engineered_region	673	752	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300010	.	stem_loop	684	727	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300010	.	terminator	761	801	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300010	.	stop_codon	794	794	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300010	.	polyA_site	788	801	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300010	.	stem_loop	768	787	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300010	.	engineered_region	761	801	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300010	.	engineered_region	1	664	.	+	0	ID=BBa_K081008;Name=BBa_K081008;Parent=BBa_K300009
BBa_K300010	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081008
BBa_K300010	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K300010	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K300010	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K300010	.	CDS	22	639	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K081008
BBa_K300010	.	CDS	22	600	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K300010	.	engineered_tag	640	664	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K300010	.	engineered_region	22	639	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K300010	.	tag	601	632	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K300010	.	engineered_region	810	1870	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_K300010	.	CDS	890	1645	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K300010	.	engineered_tag	1646	1670	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K300010	.	engineered_region	890	1645	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K300010	.	engineered_region	890	891	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K300010	.	point_mutation	1063	1063	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K300010	.	CDS	890	1639	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K300010	.	point_mutation	1381	1381	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K300010	.	ribosome_entry_site	872	883	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K300010	.	conserved_region	876	879	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K300010	.	promoter	810	863	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K300010	.	engineered_region	810	863	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K300010	.	promoter	829	834	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K300010	.	promoter	852	857	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K300010	.	non_covalent_binding_site	810	828	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K300010	.	non_covalent_binding_site	835	853	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K300010	.	terminator	1679	1807	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K300010	.	terminator	1679	1758	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300010	.	engineered_region	1679	1758	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300010	.	stem_loop	1690	1733	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300010	.	terminator	1767	1807	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300010	.	stop_codon	1800	1800	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300010	.	polyA_site	1794	1807	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300010	.	stem_loop	1774	1793	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300010	.	engineered_region	1767	1807	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300010	.	promoter	1816	1870	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K300010	.	start_codon	1868	1868	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K300010	.	promoter	1857	1862	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K300010	.	promoter	1835	1840	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K300010	.	engineered_region	1816	1870	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K300010	.	non_covalent_binding_site	1816	1835	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_K300010
attaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaag
gtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcaga
tgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttatta
cctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaata
tagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaact
atttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcga
tttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttg
tattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag
agaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtag
aagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatt
tatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacg
ctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaa
caatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccct
attcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttt
tacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataa
atcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttca
aaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgct
gccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagac
ctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa