LOCUS       BBa_K300012             1913 bp    DNA     linear       17-May-2024
DEFINITION  Self-inducible device with J23105
ACCESSION   BBa_K300012
VERSION     BBa_K300012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    644..675
                     /label=LVA
     CDS             65..643
                     /label=luxI
     misc_feature    65..682
                     /label=BBa_C0061
     misc_feature    683..707
                     /label=Help:Barcodes
     CDS             65..682
                     /label=BBa_C0061
     misc_feature    44..707
                     /label=BBa_K081008
     stem_loop       727..770
                     /label=stem_loop
     misc_feature    716..795
                     /label=BBa_B0010
     terminator      716..795
                     /label=BBa_B0010
     stem_loop       811..830
                     /label=T7 TE
     misc_feature    837..837
                     /label=stop
     polyA_site      831..844
                     /label=polya
     misc_feature    804..844
                     /label=BBa_B0012
     terminator      804..844
                     /label=BBa_B0012
     terminator      716..844
                     /label=BBa_B0015
     misc_feature    44..844
                     /label=BBa_K300009
     misc_feature    853..871
                     /label=TetR 1
     misc_feature    853..906
                     /label=BBa_R0040
     promoter        872..877
                     /label=-35
     misc_feature    878..896
                     /label=TetR 2
     promoter        895..900
                     /label=-10
     promoter        853..906
                     /label=BBa_R0040
     misc_feature    919..922
                     /label=conserved
     RBS             915..926
                     /label=BBa_B0034
     misc_feature    933..934
                     /label=prefix
     misc_feature    1106..1106
                     /label=T
     misc_feature    1424..1424
                     /label=A
     CDS             933..1682
                     /label=luxR
     misc_feature    933..1688
                     /label=BBa_C0062
     misc_feature    1689..1713
                     /label=Help:Barcodes
     CDS             933..1688
                     /label=BBa_C0062
     stem_loop       1733..1776
                     /label=stem_loop
     misc_feature    1722..1801
                     /label=BBa_B0010
     terminator      1722..1801
                     /label=BBa_B0010
     stem_loop       1817..1836
                     /label=T7 TE
     misc_feature    1843..1843
                     /label=stop
     polyA_site      1837..1850
                     /label=polya
     misc_feature    1810..1850
                     /label=BBa_B0012
     terminator      1810..1850
                     /label=BBa_B0012
     terminator      1722..1850
                     /label=BBa_B0015
     misc_feature    1859..1878
                     /label=LuxR/HSL
     promoter        1878..1883
                     /label=-35
     promoter        1900..1905
                     /label=-10
     misc_feature    1911..1911
                     /label=start
     misc_feature    1859..1913
                     /label=BBa_R0062
     promoter        1859..1913
                     /label=BBa_R0062
     misc_feature    853..1913
                     /label=BBa_F2620
     misc_feature    44..1913
                     /label=BBa_K300010
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagattaaag aggagaaata
       61 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa
      121 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt
      181 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg
      241 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat
      301 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat
      361 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag
      421 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac
      481 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc
      541 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt
      601 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa
      661 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg
      721 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      781 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      841 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact
      901 gagcactact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat
      961 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat
     1021 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt
     1081 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt
     1141 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt
     1201 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa
     1261 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta
     1321 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt
     1381 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc
     1441 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat
     1501 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca
     1561 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc
     1621 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa
     1681 attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg
     1741 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1801 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac
     1861 ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt atagtcgaat aaa
//