##gff-version 3
##sequence-region BBa_K300018 1 2797
BBa_K300018	.	engineered_region	1922	2797	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K300018	.	terminator	2757	2797	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K300018	.	stem_loop	2764	2783	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300018	.	engineered_region	2757	2797	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300018	.	stop_codon	2790	2790	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300018	.	polyA_site	2784	2797	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300018	.	terminator	2669	2748	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K300018	.	engineered_region	2669	2748	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300018	.	stem_loop	2680	2723	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300018	.	ribosome_entry_site	1922	1934	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K300018	.	engineered_region	1922	1934	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K300018	.	ribosome_entry_site	1922	1934	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K300018	.	ribosome_entry_site	1928	1931	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K300018	.	CDS	1941	2660	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K300018	.	CDS	1941	2660	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K300018	.	engineered_region	1	1913	.	+	0	ID=BBa_K300011;Name=BBa_K300011
BBa_K300018	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K300011
BBa_K300018	.	engineered_region	44	1913	.	+	0	ID=BBa_K300010;Name=BBa_K300010;Parent=BBa_K300011
BBa_K300018	.	engineered_region	44	844	.	+	0	ID=BBa_K300009;Name=BBa_K300009;Parent=BBa_K300010
BBa_K300018	.	engineered_region	44	707	.	+	0	ID=BBa_K081008;Name=BBa_K081008;Parent=BBa_K300009
BBa_K300018	.	CDS	65	682	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K081008
BBa_K300018	.	CDS	65	643	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K300018	.	engineered_region	65	682	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K300018	.	tag	644	675	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K300018	.	engineered_tag	683	707	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K300018	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081008
BBa_K300018	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K300018	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K300018	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K300018	.	terminator	716	844	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K300009
BBa_K300018	.	terminator	804	844	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300018	.	stem_loop	811	830	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300018	.	engineered_region	804	844	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300018	.	stop_codon	837	837	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300018	.	polyA_site	831	844	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300018	.	terminator	716	795	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300018	.	engineered_region	716	795	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300018	.	stem_loop	727	770	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300018	.	engineered_region	853	1913	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_K300010
BBa_K300018	.	CDS	933	1688	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K300018	.	engineered_tag	1689	1713	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K300018	.	engineered_region	933	934	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K300018	.	point_mutation	1106	1106	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K300018	.	CDS	933	1682	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K300018	.	engineered_region	933	1688	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K300018	.	point_mutation	1424	1424	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K300018	.	ribosome_entry_site	915	926	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K300018	.	conserved_region	919	922	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K300018	.	promoter	853	906	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K300018	.	promoter	872	877	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K300018	.	non_covalent_binding_site	878	896	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K300018	.	promoter	895	900	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K300018	.	engineered_region	853	906	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K300018	.	non_covalent_binding_site	853	871	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K300018	.	terminator	1722	1850	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K300018	.	terminator	1810	1850	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300018	.	stem_loop	1817	1836	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300018	.	engineered_region	1810	1850	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300018	.	stop_codon	1843	1843	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300018	.	polyA_site	1837	1850	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300018	.	terminator	1722	1801	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300018	.	engineered_region	1722	1801	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300018	.	stem_loop	1733	1776	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300018	.	promoter	1859	1913	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K300018	.	promoter	1900	1905	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K300018	.	start_codon	1911	1911	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K300018	.	promoter	1878	1883	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K300018	.	engineered_region	1859	1913	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K300018	.	non_covalent_binding_site	1859	1878	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_K300018
tttacagctagctcagtcctaggtattatgctagctactagagattaaagaggagaaatactagatgactataatgata
aaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaa
gacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgc
ttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgttttt
cctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaata
gctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaagg
tattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtatt
ggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaag
cagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgataga
gattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata