LOCUS BBa_K300021 2797 bp DNA linear 22-Dec-2024 DEFINITION Measurement system for K300014 self-inducible device ACCESSION BBa_K300021 VERSION BBa_K300021.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 644..675 /label=LVA CDS 65..643 /label=luxI misc_feature 65..682 /label=BBa_C0061 misc_feature 683..707 /label=Help:Barcodes CDS 65..682 /label=BBa_C0061 misc_feature 44..707 /label=BBa_K081008 stem_loop 727..770 /label=stem_loop misc_feature 716..795 /label=BBa_B0010 terminator 716..795 /label=BBa_B0010 stem_loop 811..830 /label=T7 TE misc_feature 837..837 /label=stop polyA_site 831..844 /label=polya misc_feature 804..844 /label=BBa_B0012 terminator 804..844 /label=BBa_B0012 terminator 716..844 /label=BBa_B0015 misc_feature 44..844 /label=BBa_K300009 misc_feature 853..871 /label=TetR 1 misc_feature 853..906 /label=BBa_R0040 promoter 872..877 /label=-35 misc_feature 878..896 /label=TetR 2 promoter 895..900 /label=-10 promoter 853..906 /label=BBa_R0040 misc_feature 919..922 /label=conserved RBS 915..926 /label=BBa_B0034 misc_feature 933..934 /label=prefix misc_feature 1106..1106 /label=T misc_feature 1424..1424 /label=A CDS 933..1682 /label=luxR misc_feature 933..1688 /label=BBa_C0062 misc_feature 1689..1713 /label=Help:Barcodes CDS 933..1688 /label=BBa_C0062 stem_loop 1733..1776 /label=stem_loop misc_feature 1722..1801 /label=BBa_B0010 terminator 1722..1801 /label=BBa_B0010 stem_loop 1817..1836 /label=T7 TE misc_feature 1843..1843 /label=stop polyA_site 1837..1850 /label=polya misc_feature 1810..1850 /label=BBa_B0012 terminator 1810..1850 /label=BBa_B0012 terminator 1722..1850 /label=BBa_B0015 misc_feature 1859..1878 /label=LuxR/HSL promoter 1878..1883 /label=-35 promoter 1900..1905 /label=-10 misc_feature 1911..1911 /label=start misc_feature 1859..1913 /label=BBa_R0062 promoter 1859..1913 /label=BBa_R0062 misc_feature 853..1913 /label=BBa_F2620 misc_feature 44..1913 /label=BBa_K300010 misc_feature 1..1913 /label=BBa_K300014 RBS 1922..1934 /label=RBS-3\Weak RBS 1928..1931 /label=RBS misc_feature 1922..1934 /label=BBa_B0032 RBS 1922..1934 /label=BBa_B0032 CDS 1941..2660 /label=GFP protein CDS 1941..2660 /label=BBa_E0040 stem_loop 2680..2723 /label=stem_loop misc_feature 2669..2748 /label=BBa_B0010 terminator 2669..2748 /label=BBa_B0010 stem_loop 2764..2783 /label=T7 TE misc_feature 2790..2790 /label=stop polyA_site 2784..2797 /label=polya misc_feature 2757..2797 /label=BBa_B0012 terminator 2757..2797 /label=BBa_B0012 misc_feature 1922..2797 /label=BBa_E0240 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagattaaag aggagaaata 61 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa 121 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt 181 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg 241 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat 301 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat 361 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag 421 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac 481 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc 541 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt 601 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa 661 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg 721 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 781 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 841 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 901 gagcactact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat 961 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat 1021 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt 1081 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt 1141 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt 1201 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa 1261 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta 1321 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt 1381 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc 1441 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat 1501 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca 1561 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc 1621 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa 1681 attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg 1741 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1801 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac 1861 ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt atagtcgaat aaatactaga 1921 gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 1981 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 2041 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 2101 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 2161 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 2221 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 2281 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 2341 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 2401 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 2461 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 2521 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 2581 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 2641 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2701 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2761 actggctcac cttcgggtgg gcctttctgc gtttata //