##gff-version 3
##sequence-region BBa_K300027 1 844
BBa_K300027	.	engineered_region	44	844	.	+	0	ID=BBa_K300009;Name=BBa_K300009
BBa_K300027	.	engineered_region	44	707	.	+	0	ID=BBa_K081008;Name=BBa_K081008;Parent=BBa_K300009
BBa_K300027	.	CDS	65	682	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K081008
BBa_K300027	.	tag	644	675	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K300027	.	CDS	65	643	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K300027	.	engineered_region	65	682	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K300027	.	engineered_tag	683	707	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K300027	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081008
BBa_K300027	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K300027	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K300027	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K300027	.	terminator	716	844	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K300009
BBa_K300027	.	terminator	716	795	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300027	.	stem_loop	727	770	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300027	.	engineered_region	716	795	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300027	.	terminator	804	844	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300027	.	polyA_site	831	844	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300027	.	stop_codon	837	837	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300027	.	engineered_region	804	844	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300027	.	stem_loop	811	830	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300027	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
>BBa_K300027
tttacggctagctcagtcctaggtatagtgctagctactagagattaaagaggagaaatactagatgactataatgata
aaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaa
gacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgc
ttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgttttt
cctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaata
gctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaagg
tattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtatt
ggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaag
cagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata