##gff-version 3
##sequence-region BBa_K300031 1 825
BBa_K300031	.	engineered_region	1	688	.	+	0	ID=BBa_K081009;Name=BBa_K081009
BBa_K300031	.	CDS	22	688	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081009
BBa_K300031	.	tag	625	657	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K300031	.	CDS	22	624	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K300031	.	engineered_tag	664	688	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K300031	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081009
BBa_K300031	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K300031	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K300031	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K300031	.	terminator	697	825	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K300031	.	terminator	785	825	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300031	.	polyA_site	812	825	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300031	.	stem_loop	792	811	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300031	.	engineered_region	785	825	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300031	.	stop_codon	818	818	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300031	.	terminator	697	776	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300031	.	stem_loop	708	751	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300031	.	engineered_region	697	776	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K300031
attaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgc
acaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggtta
cgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttc
gacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcaca
tctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctat
gcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatg
atgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgta
tcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaa
cgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata