LOCUS       BBa_K300085             1436 bp    DNA     linear       01-Jun-2024
DEFINITION  BBa_K300086 construction intermediate
ACCESSION   BBa_K300085
VERSION     BBa_K300085.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     misc_feature    1..576
                     /label=BBa_K300002
     misc_feature    577..582
                     /label=BBa_K300077
     CDS             583..1300
                     /label=GFP fusion brick
     misc_feature    583..583
                     /label=deletion of TG
     CDS             583..1300
                     /label=BBa_K125500
     misc_feature    1301..1307
                     /label=BBa_K300076
     stem_loop       1319..1362
                     /label=stem_loop
     misc_feature    1308..1387
                     /label=BBa_B0010
     terminator      1308..1387
                     /label=BBa_B0010
     stem_loop       1403..1422
                     /label=T7 TE
     misc_feature    1429..1429
                     /label=stop
     polyA_site      1423..1436
                     /label=polya
     misc_feature    1396..1436
                     /label=BBa_B0012
     terminator      1396..1436
                     /label=BBa_B0012
     terminator      1308..1436
                     /label=BBa_B0015
     misc_feature    583..1436
                     /label=BBa_K300005
ORIGIN
        1 atgatcctca ccccggaaca agttgcagca gcgcaaaagg ccaacctcga aacgctgttc
       61 ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg tcgagctgaa ccttcaggtc
      121 gtcaagactt cgttcgcaga aggcgttgac aacgccaaga aggcgctgtc ggccaaggac
      181 gcacaggaac tgctggccat ccaggccgca gccgtgcagc cggttgccga aaagaccctg
      241 gcctacaccc gccacctgta tgaaatcgct tcggaaaccc agagcgagtt caccaaggta
      301 gccgaggctc aactggccga aggctcgaag aacgtgcaag cgctggtcga gaacctcgcc
      361 aagaacgccc cggccggttc ggaatcgacc gtggccatcg tgaagtcggc gatctccgct
      421 gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc aagcggtcga aatcgctgaa
      481 accaacttcc aggctgcggc tacggctgcc accaaggctg cccagcaagc cagcgccacg
      541 gcccgtacgg ccacggcaaa gaagacgacg gctgccacta gacgtaaagg agaagaactt
      601 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      661 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      721 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      781 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      841 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      901 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      961 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     1021 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     1081 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1141 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1201 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1261 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     1321 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1381 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//