LOCUS       BBa_K300086             1516 bp    DNA     linear       29-Nov-2024
DEFINITION  Measurement system for BBa_K300002
ACCESSION   BBa_K300086
VERSION     BBa_K300086.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=Start
     misc_feature    81..656
                     /label=BBa_K300002
     misc_feature    657..662
                     /label=BBa_K300077
     CDS             663..1380
                     /label=GFP fusion brick
     misc_feature    663..663
                     /label=deletion of TG
     CDS             663..1380
                     /label=BBa_K125500
     misc_feature    1381..1387
                     /label=BBa_K300076
     stem_loop       1399..1442
                     /label=stem_loop
     misc_feature    1388..1467
                     /label=BBa_B0010
     terminator      1388..1467
                     /label=BBa_B0010
     stem_loop       1483..1502
                     /label=T7 TE
     misc_feature    1509..1509
                     /label=stop
     polyA_site      1503..1516
                     /label=polya
     misc_feature    1476..1516
                     /label=BBa_B0012
     terminator      1476..1516
                     /label=BBa_B0012
     terminator      1388..1516
                     /label=BBa_B0015
     misc_feature    663..1516
                     /label=BBa_K300005
     misc_feature    81..1516
                     /label=BBa_K300085
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      121 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      181 tcgagctgaa ccttcaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga
      241 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc
      301 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc
      361 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag
      421 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg
      481 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc
      541 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg
      601 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgccacta
      661 gacgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      721 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      781 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      841 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      901 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      961 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1021 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1081 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1141 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1201 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1261 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1321 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1381 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1441 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1501 cctttctgcg tttata
//