##gff-version 3
##sequence-region BBa_K300086 1 1516
BBa_K300086	.	engineered_region	81	1516	.	+	0	ID=BBa_K300085;Name=BBa_K300085
BBa_K300086	.	sequence_feature	657	662	.	+	0	ID=BBa_K300077;Name=BBa_K300077;Parent=BBa_K300085
BBa_K300086	.	engineered_region	663	1516	.	+	0	ID=BBa_K300005;Name=BBa_K300005;Parent=BBa_K300085
BBa_K300086	.	terminator	1388	1516	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K300005
BBa_K300086	.	terminator	1388	1467	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300086	.	stem_loop	1399	1442	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300086	.	engineered_region	1388	1467	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300086	.	terminator	1476	1516	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300086	.	stem_loop	1483	1502	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300086	.	stop_codon	1509	1509	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300086	.	polyA_site	1503	1516	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300086	.	engineered_region	1476	1516	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300086	.	CDS	663	1380	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K300005
BBa_K300086	.	CDS	663	1380	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_K300086	.	point_mutation	663	663	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_K300086	.	sequence_feature	1381	1387	.	+	0	ID=BBa_K300076;Name=BBa_K300076;Parent=BBa_K300005
BBa_K300086	.	polypeptide_domain	81	656	.	+	0	ID=BBa_K300002;Name=BBa_K300002;Parent=BBa_K300085
BBa_K300086	.	start_codon	81	83	.	+	0	ID=annotation2081800;Name=Start;Parent=BBa_K300002
BBa_K300086	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_K300086	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K300086	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K300086	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K300086	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K300086	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K300086	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K300086	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K300086	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_K300086
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcg
tttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgcca
agaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagac
cctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactg
gccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtgg
ccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaat
cgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggcc
acggcaaagaagacgacggctgccactagacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaat
tagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccct
taaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgc
tttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaa
ctatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaat
cgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaat
gtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcg
ttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtc
cacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggg
attacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata