LOCUS       BBa_K300087             2018 bp    DNA     linear       22-Nov-2024
DEFINITION  BBa_K300088 construction intermediate
ACCESSION   BBa_K300087
VERSION     BBa_K300087.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     misc_feature    1..576
                     /label=BBa_K300002
     misc_feature    577..582
                     /label=BBa_K300077
     misc_feature    583..1158
                     /label=BBa_K300003
     misc_feature    1159..1164
                     /label=BBa_K300077
     CDS             1165..1882
                     /label=GFP fusion brick
     misc_feature    1165..1165
                     /label=deletion of TG
     CDS             1165..1882
                     /label=BBa_K125500
     misc_feature    1883..1889
                     /label=BBa_K300076
     stem_loop       1901..1944
                     /label=stem_loop
     misc_feature    1890..1969
                     /label=BBa_B0010
     terminator      1890..1969
                     /label=BBa_B0010
     stem_loop       1985..2004
                     /label=T7 TE
     misc_feature    2011..2011
                     /label=stop
     polyA_site      2005..2018
                     /label=polya
     misc_feature    1978..2018
                     /label=BBa_B0012
     terminator      1978..2018
                     /label=BBa_B0012
     terminator      1890..2018
                     /label=BBa_B0015
     misc_feature    1165..2018
                     /label=BBa_K300005
ORIGIN
        1 atgatcctca ccccggaaca agttgcagca gcgcaaaagg ccaacctcga aacgctgttc
       61 ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg tcgagctgaa ccttcaggtc
      121 gtcaagactt cgttcgcaga aggcgttgac aacgccaaga aggcgctgtc ggccaaggac
      181 gcacaggaac tgctggccat ccaggccgca gccgtgcagc cggttgccga aaagaccctg
      241 gcctacaccc gccacctgta tgaaatcgct tcggaaaccc agagcgagtt caccaaggta
      301 gccgaggctc aactggccga aggctcgaag aacgtgcaag cgctggtcga gaacctcgcc
      361 aagaacgccc cggccggttc ggaatcgacc gtggccatcg tgaagtcggc gatctccgct
      421 gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc aagcggtcga aatcgctgaa
      481 accaacttcc aggctgcggc tacggctgcc accaaggctg cccagcaagc cagcgccacg
      541 gcccgtacgg ccacggcaaa gaagacgacg gctgccacta gaatgatcct caccccggaa
      601 caagttgcag cagcgcaaaa ggccaacctc gaaacgctgt tcggcctgac caccaaggcg
      661 tttgaaggcg tcgaaaagct cgtcgagctg aaccttcagg tcgtcaagac ttcgttcgca
      721 gaaggcgttg acaacgccaa gaaggcgctg tcggccaagg acgcacagga actgctggcc
      781 atccaggccg cagccgtgca gccggttgcc gaaaagaccc tggcctacac ccgccacctg
      841 tatgaaatcg cttcggaaac ccagagcgag ttcaccaagg tagccgaggc tcaactggcc
      901 gaaggctcga agaacgtgca agcgctggtc gagaacctcg ccaagaacgc cccggccggt
      961 tcggaatcga ccgtggccat cgtgaagtcg gcgatctccg ctgccaacaa cgcctacgag
     1021 tcggtgcaga aggcgaccaa gcaagcggtc gaaatcgctg aaaccaactt ccaggctgcg
     1081 gctacggctg ccaccaaggc tgcccagcaa gccagcgcca cggcccgtac ggccacggca
     1141 aagaagacga cggctgccac tagacgtaaa ggagaagaac ttttcactgg agttgtccca
     1201 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     1261 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1321 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1381 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1441 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1501 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1561 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1621 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1681 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1741 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1801 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1861 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1981 cactggctca ccttcgggtg ggcctttctg cgtttata
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