LOCUS       BBa_K300088             2098 bp    DNA     linear       25-Nov-2024
DEFINITION  Measurement system for BBa_K300093
ACCESSION   BBa_K300088
VERSION     BBa_K300088.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=Start
     misc_feature    81..656
                     /label=BBa_K300002
     misc_feature    657..662
                     /label=BBa_K300077
     misc_feature    663..1238
                     /label=BBa_K300003
     misc_feature    1239..1244
                     /label=BBa_K300077
     CDS             1245..1962
                     /label=GFP fusion brick
     misc_feature    1245..1245
                     /label=deletion of TG
     CDS             1245..1962
                     /label=BBa_K125500
     misc_feature    1963..1969
                     /label=BBa_K300076
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
     terminator      1970..2098
                     /label=BBa_B0015
     misc_feature    1245..2098
                     /label=BBa_K300005
     misc_feature    81..2098
                     /label=BBa_K300087
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      121 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      181 tcgagctgaa ccttcaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga
      241 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc
      301 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc
      361 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag
      421 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg
      481 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc
      541 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg
      601 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgccacta
      661 gaatgatcct caccccggaa caagttgcag cagcgcaaaa ggccaacctc gaaacgctgt
      721 tcggcctgac caccaaggcg tttgaaggcg tcgaaaagct cgtcgagctg aaccttcagg
      781 tcgtcaagac ttcgttcgca gaaggcgttg acaacgccaa gaaggcgctg tcggccaagg
      841 acgcacagga actgctggcc atccaggccg cagccgtgca gccggttgcc gaaaagaccc
      901 tggcctacac ccgccacctg tatgaaatcg cttcggaaac ccagagcgag ttcaccaagg
      961 tagccgaggc tcaactggcc gaaggctcga agaacgtgca agcgctggtc gagaacctcg
     1021 ccaagaacgc cccggccggt tcggaatcga ccgtggccat cgtgaagtcg gcgatctccg
     1081 ctgccaacaa cgcctacgag tcggtgcaga aggcgaccaa gcaagcggtc gaaatcgctg
     1141 aaaccaactt ccaggctgcg gctacggctg ccaccaaggc tgcccagcaa gccagcgcca
     1201 cggcccgtac ggccacggca aagaagacga cggctgccac tagacgtaaa ggagaagaac
     1261 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     1321 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     1381 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     1441 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     1501 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     1561 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     1621 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     1681 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     1741 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1801 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1861 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1921 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//