BBa_K300089
type: ComponentDefinition
persistent identity: BBa K300089
display id: BBa_K300089
version: 1
was generated by: iGEM to SBOL conversion
description: BBa_K300090 construction intermediate
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
mutable description: This part was used to create <partinfo>BBa_K300090</partinfo> BioBrick measurement system / device.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 4140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Giacomo Zambianchi, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K300077
component: BBa_K105012
component: BBa_K300002
component: BBa_K300005
component: BBa_K300003
sequence annotation: BBa_K300002
sequence annotation: BBa_K300077
sequence annotation: BBa_K105012
sequence annotation: BBa_K300003
sequence annotation: BBa_K300005
sequence: https://synbiohub.org/public/igem/BBa_K300089_sequence/1

BBa_K300077
type: Component
persistent identity: component2089707
display id: component2089707
version: 1
top level: BBa_K300089
definition: scar
access: public

BBa_K105012
type: Component
persistent identity: component2089706
display id: component2089706
version: 1
top level: BBa_K300089
definition: BBa_K105012
access: public

BBa_K300077
type: Component
persistent identity: component2089705
display id: component2089705
version: 1
top level: BBa_K300089
definition: scar
access: public

BBa_K300002
type: Component
persistent identity: component2089704
display id: component2089704
version: 1
top level: BBa_K300089
definition: BBa_K300002
access: public

BBa_K300005
type: Component
persistent identity: component2089720
display id: component2089720
version: 1
top level: BBa_K300089
definition: BBa_K300005
access: public

BBa_K300077
type: Component
persistent identity: component2089709
display id: component2089709
version: 1
top level: BBa_K300089
definition: scar
access: public

BBa_K300003
type: Component
persistent identity: component2089708
display id: component2089708
version: 1
top level: BBa_K300089
definition: BBa_K300003
access: public

BBa_K300002
type: SequenceAnnotation
persistent identity: annotation2089704
display id: annotation2089704
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089704/range2089704/1
component: BBa_K300002

https://synbiohub.org/public/igem/BBa_K300089/annotation2089704/range2089704/1
type: Range
persistent identity: range2089704
display id: range2089704
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 1
end: 576
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2089705
display id: annotation2089705
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089705/range2089705/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300089/annotation2089705/range2089705/1
type: Range
persistent identity: range2089705
display id: range2089705
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 577
end: 582
orientation: inline

BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2089706
display id: annotation2089706
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089706/range2089706/1
component: BBa_K105012

https://synbiohub.org/public/igem/BBa_K300089/annotation2089706/range2089706/1
type: Range
persistent identity: range2089706
display id: range2089706
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 583
end: 612
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2089707
display id: annotation2089707
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089707/range2089707/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300089/annotation2089707/range2089707/1
type: Range
persistent identity: range2089707
display id: range2089707
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 613
end: 618
orientation: inline

BBa_K300003
type: SequenceAnnotation
persistent identity: annotation2089708
display id: annotation2089708
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089708/range2089708/1
component: BBa_K300003

https://synbiohub.org/public/igem/BBa_K300089/annotation2089708/range2089708/1
type: Range
persistent identity: range2089708
display id: range2089708
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 619
end: 1194
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2089709
display id: annotation2089709
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089709/range2089709/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300089/annotation2089709/range2089709/1
type: Range
persistent identity: range2089709
display id: range2089709
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 1195
end: 1200
orientation: inline

BBa_K300005
type: SequenceAnnotation
persistent identity: annotation2089720
display id: annotation2089720
version: 1
top level: BBa_K300089
location: https://synbiohub.org/public/igem/BBa_K300089/annotation2089720/range2089720/1
component: BBa_K300005

https://synbiohub.org/public/igem/BBa_K300089/annotation2089720/range2089720/1
type: Range
persistent identity: range2089720
display id: range2089720
version: 1
top level: BBa_K300089
direction: direction/reverse
start: 1201
end: 2054
orientation: inline

BBa_K105012
type: ComponentDefinition
persistent identity: BBa K105012
display id: BBa_K105012
version: 1
was generated by: iGEM to SBOL conversion
description: 10 aa flexible protein domain linker
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered <br\n> The amino acide sequence corresponds to the interdomaine linker of xxx. ''paper''
owned by: james
owned by: myers
mutable description: This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled. <br\n>
discontinued: false
dominant: true
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
star: true
creator: Manuel Gersbacher, Katja Karstens
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_K105012_sequence/1

BBa_K300002
type: ComponentDefinition
persistent identity: BBa K300002
display id: BBa_K300002
version: 1
was generated by: iGEM to SBOL conversion
description: Phasin (PhaP) - head domain
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: Ralstonia eutropha genomic DNA.
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It is identical to <partinfo>BBa_K208001</partinfo>, but it lacks the stop codon in order to support protein fusions. It has been designed as a head domain: The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix) The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix) For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions. CONSTRUCTION METHOD: <partinfo>BBa_K208001</partinfo> (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers: phaP10F 5'-GCTTCTAGATGATCCTCACCCCGGAACA-3' phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3' in order to delete the stop codon. The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with <partinfo>pSB1AK3</partinfo> (previously cut with XbaI-SpeI and dephosphorylated). Positive clones were found through digestion screening/sequencing.
star: false
creator: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K300002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2081800
display id: annotation2081800
version: 1
top level: BBa_K300002
location: https://synbiohub.org/public/igem/BBa_K300002/annotation2081800/range2081800/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K300002/annotation2081800/range2081800/1
type: Range
persistent identity: range2081800
display id: range2081800
version: 1
top level: BBa_K300002
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K300003
type: ComponentDefinition
persistent identity: BBa K300003
display id: BBa_K300003
version: 1
was generated by: iGEM to SBOL conversion
description: Phasin (PhaP) - internal domain
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: It is identical to <partinfo>BBa_K208001</partinfo>, but it lacks the stop codon in order to support protein fusions. It has been designed as a head domain: The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix) The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix) For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions. '''CONSTRUCTION METHOD:''' <partinfo>BBa_K208001</partinfo> (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers: phaPSF: 5'GCTTCTAGAATGATCCTCACCCCGGAACA-3' phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3' in order to delete the stop codon. The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with <partinfo>pSB1A3</partinfo> (previously cut with XbaI-SpeI and dephosphorylated). Positive clones were found through digestion screening/sequencing.
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Ralstonia eutropha genomic DNA.
star: false
creator: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K300003_sequence/1

BBa_K300005
type: ComponentDefinition
persistent identity: BBa K300005
display id: BBa_K300005
version: 1
was generated by: iGEM to SBOL conversion
description: GFP (Silver Standard Prefix) with terminator
created: 2010-10-03T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: <partinfo>K125500</partinfo> - from 2010 DNA Distribution; <partinfo>BBa_B0015</partinfo> - from 2009 DNA Distribution.
owned by: james
owned by: myers
mutable description: This part can be useful to construct fluorescent fusion proteins. It is composed by a tail domain (the GFP <partinfo>K125500</partinfo>) with a transcriptional terminator (<partinfo>BBa_B0015</partinfo>) downstream. Other protein domains can be fused upstream of this part in order to create chimeric fluorescent proteins.
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: false
creator: Giacomo Zambianchi, Sara Bisi, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K300076
component: BBa_K125500
component: BBa_B0015
sequence annotation: BBa_K125500
sequence annotation: BBa_K300076
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K300005_sequence/1

BBa_K300076
type: Component
persistent identity: component2089640
display id: component2089640
version: 1
top level: BBa_K300005
definition: scar
access: public

BBa_K125500
type: Component
persistent identity: component2089639
display id: component2089639
version: 1
top level: BBa_K300005
definition: BBa_K125500
access: public

BBa_B0015
type: Component
persistent identity: component2089647
display id: component2089647
version: 1
top level: BBa_K300005
definition: BBa_B0015
access: public

BBa_K125500
type: SequenceAnnotation
persistent identity: annotation2089639
display id: annotation2089639
version: 1
top level: BBa_K300005
location: https://synbiohub.org/public/igem/BBa_K300005/annotation2089639/range2089639/1
component: BBa_K125500

https://synbiohub.org/public/igem/BBa_K300005/annotation2089639/range2089639/1
type: Range
persistent identity: range2089639
display id: range2089639
version: 1
top level: BBa_K300005
direction: direction/reverse
start: 1
end: 718
orientation: inline

BBa_K300076
type: SequenceAnnotation
persistent identity: annotation2089640
display id: annotation2089640
version: 1
top level: BBa_K300005
location: https://synbiohub.org/public/igem/BBa_K300005/annotation2089640/range2089640/1
component: BBa_K300076

https://synbiohub.org/public/igem/BBa_K300005/annotation2089640/range2089640/1
type: Range
persistent identity: range2089640
display id: range2089640
version: 1
top level: BBa_K300005
direction: direction/reverse
start: 719
end: 725
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2089647
display id: annotation2089647
version: 1
top level: BBa_K300005
location: https://synbiohub.org/public/igem/BBa_K300005/annotation2089647/range2089647/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K300005/annotation2089647/range2089647/1
type: Range
persistent identity: range2089647
display id: range2089647
version: 1
top level: BBa_K300005
direction: direction/reverse
start: 726
end: 854
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa K300077
display id: BBa_K300077
version: 1
was generated by: iGEM to SBOL conversion
description: RFC23 scar sequence
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
mutable description: RFC23 scar sequence (Silver-Silver mixed site)
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 4140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Giacomo Zambianchi, Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300077_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K125500
type: ComponentDefinition
persistent identity: BBa K125500
display id: BBa_K125500
version: 1
was generated by: iGEM to SBOL conversion
description: GFP fusion brick
created: 2008-07-24T11:00:00Z
modified: 2016-01-25T02:34:56Z
mutable provenance: The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The first two nucleotides of the GFP start codon were removed to create this fusion brick.
star: false
creator: Grace Kwan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP fusion brick
sequence annotation: deletion of TG
sequence: https://synbiohub.org/public/igem/BBa_K125500_sequence/1

GFP fusion brick
type: SequenceAnnotation
persistent identity: annotation1968260
display id: annotation1968260
version: 1
top level: BBa_K125500
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
type: Range
persistent identity: range1968260
display id: range1968260
version: 1
top level: BBa_K125500
direction: direction/forward
start: 1
end: 718
orientation: inline

deletion of TG
type: SequenceAnnotation
persistent identity: annotation1969908
display id: annotation1969908
version: 1
top level: BBa_K125500
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
type: Range
persistent identity: range1969908
display id: range1969908
version: 1
top level: BBa_K125500
direction: direction/forward
start: 1
end: 1
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa K300076
display id: BBa_K300076
version: 1
was generated by: iGEM to SBOL conversion
description: RFC23-RFC10 mixed scar
created: 2010-10-18T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
mutable description: This is the scar that you get when a Standard assembly compliant BioBrick to a Silver standard compliant BioBrick
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 4140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Giacomo Zambianchi, Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300076_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K300089_sequence/1
type: Sequence
persistent identity: BBa K300089 sequence
display id: BBa_K300089_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggtactagaatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105012_sequence/1
type: Sequence
persistent identity: BBa K105012 sequence
display id: BBa_K105012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgaaaatttgtattttcaatctggtggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300002_sequence/1
type: Sequence
persistent identity: BBa K300002 sequence
display id: BBa_K300002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300003_sequence/1
type: Sequence
persistent identity: BBa K300003 sequence
display id: BBa_K300003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300005_sequence/1
type: Sequence
persistent identity: BBa K300005 sequence
display id: BBa_K300005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300077_sequence/1
type: Sequence
persistent identity: BBa K300077 sequence
display id: BBa_K300077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125500_sequence/1
type: Sequence
persistent identity: BBa K125500 sequence
display id: BBa_K125500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300076_sequence/1
type: Sequence
persistent identity: BBa K300076 sequence
display id: BBa_K300076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z