LOCUS       BBa_K300090             2134 bp    DNA     linear       22-Dec-2024
DEFINITION  Measurement system for BBa_K300094
ACCESSION   BBa_K300090
VERSION     BBa_K300090.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=Start
     misc_feature    81..656
                     /label=BBa_K300002
     misc_feature    657..662
                     /label=BBa_K300077
     misc_feature    663..692
                     /label=BBa_K105012
     misc_feature    693..698
                     /label=BBa_K300077
     misc_feature    699..1274
                     /label=BBa_K300003
     misc_feature    1275..1280
                     /label=BBa_K300077
     CDS             1281..1998
                     /label=GFP fusion brick
     misc_feature    1281..1281
                     /label=deletion of TG
     CDS             1281..1998
                     /label=BBa_K125500
     misc_feature    1999..2005
                     /label=BBa_K300076
     stem_loop       2017..2060
                     /label=stem_loop
     misc_feature    2006..2085
                     /label=BBa_B0010
     terminator      2006..2085
                     /label=BBa_B0010
     stem_loop       2101..2120
                     /label=T7 TE
     misc_feature    2127..2127
                     /label=stop
     polyA_site      2121..2134
                     /label=polya
     misc_feature    2094..2134
                     /label=BBa_B0012
     terminator      2094..2134
                     /label=BBa_B0012
     terminator      2006..2134
                     /label=BBa_B0015
     misc_feature    1281..2134
                     /label=BBa_K300005
     misc_feature    81..2134
                     /label=BBa_K300089
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      121 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      181 tcgagctgaa ccttcaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga
      241 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc
      301 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc
      361 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag
      421 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg
      481 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc
      541 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg
      601 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgccacta
      661 gaggtgaaaa tttgtatttt caatctggtg gtactagaat gatcctcacc ccggaacaag
      721 ttgcagcagc gcaaaaggcc aacctcgaaa cgctgttcgg cctgaccacc aaggcgtttg
      781 aaggcgtcga aaagctcgtc gagctgaacc ttcaggtcgt caagacttcg ttcgcagaag
      841 gcgttgacaa cgccaagaag gcgctgtcgg ccaaggacgc acaggaactg ctggccatcc
      901 aggccgcagc cgtgcagccg gttgccgaaa agaccctggc ctacacccgc cacctgtatg
      961 aaatcgcttc ggaaacccag agcgagttca ccaaggtagc cgaggctcaa ctggccgaag
     1021 gctcgaagaa cgtgcaagcg ctggtcgaga acctcgccaa gaacgccccg gccggttcgg
     1081 aatcgaccgt ggccatcgtg aagtcggcga tctccgctgc caacaacgcc tacgagtcgg
     1141 tgcagaaggc gaccaagcaa gcggtcgaaa tcgctgaaac caacttccag gctgcggcta
     1201 cggctgccac caaggctgcc cagcaagcca gcgccacggc ccgtacggcc acggcaaaga
     1261 agacgacggc tgccactaga cgtaaaggag aagaactttt cactggagtt gtcccaattc
     1321 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1381 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1441 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1501 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1561 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     1621 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1681 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1741 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1801 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1861 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1921 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1981 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     2041 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     2101 ggctcacctt cgggtgggcc tttctgcgtt tata
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