LOCUS BBa_K300091 3072 bp DNA linear 22-Dec-2024 DEFINITION 3OC6HSL inducible generator for BBa_K300095 ACCESSION BBa_K300091 VERSION BBa_K300091.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 misc_feature 1088..1090 /label=Start misc_feature 1088..1663 /label=BBa_K300002 misc_feature 1..1663 /label=BBa_K300082 misc_feature 1664..1669 /label=BBa_K300077 misc_feature 1670..1699 /label=BBa_K105012 misc_feature 1700..1705 /label=BBa_K300077 misc_feature 1706..2212 /label=BBa_K300004 misc_feature 2213..2218 /label=BBa_K300077 CDS 2219..2936 /label=GFP fusion brick misc_feature 2219..2219 /label=deletion of TG CDS 2219..2936 /label=BBa_K125500 misc_feature 2937..2943 /label=BBa_K300076 stem_loop 2955..2998 /label=stem_loop misc_feature 2944..3023 /label=BBa_B0010 terminator 2944..3023 /label=BBa_B0010 stem_loop 3039..3058 /label=T7 TE misc_feature 3065..3065 /label=stop polyA_site 3059..3072 /label=polya misc_feature 3032..3072 /label=BBa_B0012 terminator 3032..3072 /label=BBa_B0012 terminator 2944..3072 /label=BBa_B0015 misc_feature 2219..3072 /label=BBa_K300005 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg atcctcaccc cggaacaagt tgcagcagcg caaaaggcca acctcgaaac 1141 gctgttcggc ctgaccacca aggcgtttga aggcgtcgaa aagctcgtcg agctgaacct 1201 tcaggtcgtc aagacttcgt tcgcagaagg cgttgacaac gccaagaagg cgctgtcggc 1261 caaggacgca caggaactgc tggccatcca ggccgcagcc gtgcagccgg ttgccgaaaa 1321 gaccctggcc tacacccgcc acctgtatga aatcgcttcg gaaacccaga gcgagttcac 1381 caaggtagcc gaggctcaac tggccgaagg ctcgaagaac gtgcaagcgc tggtcgagaa 1441 cctcgccaag aacgccccgg ccggttcgga atcgaccgtg gccatcgtga agtcggcgat 1501 ctccgctgcc aacaacgcct acgagtcggt gcagaaggcg accaagcaag cggtcgaaat 1561 cgctgaaacc aacttccagg ctgcggctac ggctgccacc aaggctgccc agcaagccag 1621 cgccacggcc cgtacggcca cggcaaagaa gacgacggct gccactagag gtgaaaattt 1681 gtattttcaa tctggtggta ctagagcact ggctgaagga acccgtattt ttgatccggt 1741 gacaggcaca actcatcgta tcgaggatgt ggttggtggg cgtaaaccga ttcatgtggt 1801 ggcagctgct aaagatggta cactgcatgc acgtccggta gtaagctggt tcgatcaggg 1861 tacacgtgat gtgattggac tgcgtattgc cggtggcgct attctgtggg ctactcctga 1921 ccataaagtc ctgacggaat atggatggcg tgctgctggt gaactgcgca aaggtgaccg 1981 tgttgctcaa cctcgtcgtt tcgatggttt tggcgattct gccccaattc ctgctcgtgt 2041 tcaggctctg gctgatgccc tggatgacaa atttctgcac gatatgctgg ccgaagaact 2101 gcgttattca gtgatccgtg aagtcctgcc tacacgtcgt gctcgtacat ttggtctgga 2161 agtggaagag ctgcatacac tggttgctga gggtgttgta gtacacaatt gcactagacg 2221 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt 2281 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact 2341 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac 2401 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga 2461 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga 2521 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag 2581 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga 2641 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa 2701 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta 2761 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc 2821 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga 2881 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta 2941 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3001 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3061 tctgcgttta ta //