##gff-version 3
##sequence-region BBa_K300091 1 3072
BBa_K300091	.	engineered_region	1	1663	.	+	0	ID=BBa_K300082;Name=BBa_K300082
BBa_K300091	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_K300082
BBa_K300091	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K300091	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K300091	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K300091	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K300091	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K300091	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K300091	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K300091	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300091	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300091	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300091	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300091	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300091	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300091	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300091	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300091	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K300091	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K300091	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K300091	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K300091	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K300091	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K300091	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K300091	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K300091	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K300091	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K300091	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K300091	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K300091	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K300091	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K300091	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K300091	.	polypeptide_domain	1088	1663	.	+	0	ID=BBa_K300002;Name=BBa_K300002;Parent=BBa_K300082
BBa_K300091	.	start_codon	1088	1090	.	+	0	ID=annotation2081800;Name=Start;Parent=BBa_K300002
BBa_K300091	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K300082
BBa_K300091	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K300091	.	polypeptide_domain	1706	2212	.	+	0	ID=BBa_K300004;Name=BBa_K300004
BBa_K300091	.	engineered_region	2219	3072	.	+	0	ID=BBa_K300005;Name=BBa_K300005
BBa_K300091	.	sequence_feature	2937	2943	.	+	0	ID=BBa_K300076;Name=BBa_K300076;Parent=BBa_K300005
BBa_K300091	.	CDS	2219	2936	.	+	0	ID=BBa_K125500;Name=BBa_K125500;Parent=BBa_K300005
BBa_K300091	.	CDS	2219	2936	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_K300091	.	point_mutation	2219	2219	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_K300091	.	terminator	2944	3072	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K300005
BBa_K300091	.	terminator	3032	3072	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K300091	.	polyA_site	3059	3072	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K300091	.	stop_codon	3065	3065	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K300091	.	stem_loop	3039	3058	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K300091	.	engineered_region	3032	3072	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K300091	.	terminator	2944	3023	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K300091	.	stem_loop	2955	2998	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K300091	.	engineered_region	2944	3023	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K300091	.	sequence_feature	1664	1669	.	+	0	ID=BBa_K300077;Name=BBa_K300077
BBa_K300091	.	sequence_feature	2213	2218	.	+	0	ID=BBa_K300077;Name=BBa_K300077
BBa_K300091	.	sequence_feature	1700	1705	.	+	0	ID=BBa_K300077;Name=BBa_K300077
BBa_K300091	.	tag	1670	1699	.	+	0	ID=BBa_K105012;Name=BBa_K105012
>BBa_K300091
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgatcctcaccccggaac
aagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagct
cgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaag
gacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacc
tgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgt
gcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatc
tccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccagg
ctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggc
tgccactagaggtgaaaatttgtattttcaatctggtggtactagagcactggctgaaggaacccgtatttttgatccg
gtgacaggcacaactcatcgtatcgaggatgtggttggtgggcgtaaaccgattcatgtggtggcagctgctaaagatg
gtacactgcatgcacgtccggtagtaagctggttcgatcagggtacacgtgatgtgattggactgcgtattgccggtgg
cgctattctgtgggctactcctgaccataaagtcctgacggaatatggatggcgtgctgctggtgaactgcgcaaaggt
gaccgtgttgctcaacctcgtcgtttcgatggttttggcgattctgccccaattcctgctcgtgttcaggctctggctg
atgccctggatgacaaatttctgcacgatatgctggccgaagaactgcgttattcagtgatccgtgaagtcctgcctac
acgtcgtgctcgtacatttggtctggaagtggaagagctgcatacactggttgctgagggtgttgtagtacacaattgc
actagacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata