BBa_K300095 1 BBa_K300095 Phasin and intein separated by a flexible protein domain linker 2010-10-21T11:00:00Z 2015-05-08T01:11:51Z a false false _420_ 0 4140 9 Not in stock true false Giacomo Zambianchi, Alessandro Ranieri, Paolo Magni component2092411 1 BBa_K300004 component2092408 1 BBa_K300077 component2092409 1 BBa_K105012 component2092410 1 BBa_K300077 component2092407 1 BBa_K300002 annotation2092409 1 BBa_K105012 range2092409 1 583 612 annotation2092408 1 BBa_K300077 range2092408 1 577 582 annotation2092407 1 BBa_K300002 range2092407 1 1 576 annotation2092411 1 BBa_K300004 range2092411 1 619 1125 annotation2092410 1 BBa_K300077 range2092410 1 613 618 BBa_K300077 1 scar RFC23 scar sequence 2010-10-16T11:00:00Z 2015-05-08T01:11:51Z RFC23 scar sequence (Silver-Silver mixed site) false true _420_ 0 4140 9 Not in stock false false Giacomo Zambianchi, Lorenzo Pasotti, Paolo Magni BBa_K300004 1 Intein Engineered pH-inducible intein (codon optimized for E. coli) - internal domain 2010-10-02T11:00:00Z 2015-05-08T01:11:51Z The original sequence comes from M. tuberculosis genomic DNA (RecA gene), but it has been highly engineered (according to [1]) to create a pH-inducible protein domain. - false false _420_ 0 2621 9 It's complicated true Codon optimized for E. coli. The amino acid sequence comes from [1]. false Sara Bisi, Paolo Magni BBa_K105012 1 BBa_K105012 10 aa flexible protein domain linker 2008-10-18T11:00:00Z 2015-05-08T01:08:52Z Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered <br\n> The amino acide sequence corresponds to the interdomaine linker of xxx. ''paper'' This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled. <br\n> false true _253_ 0 3313 9 In stock true To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006). true Manuel Gersbacher, Katja Karstens BBa_K300002 1 BBa_K300002 Phasin (PhaP) - head domain 2010-10-02T11:00:00Z 2015-05-08T01:11:51Z Ralstonia eutropha genomic DNA. - false true _420_ 0 2621 9 It's complicated true It is identical to <partinfo>BBa_K208001</partinfo>, but it lacks the stop codon in order to support protein fusions. It has been designed as a head domain: The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix) The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix) For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions. CONSTRUCTION METHOD: <partinfo>BBa_K208001</partinfo> (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers: phaP10F 5'-GCTTCTAGATGATCCTCACCCCGGAACA-3' phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3' in order to delete the stop codon. The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with <partinfo>pSB1AK3</partinfo> (previously cut with XbaI-SpeI and dephosphorylated). Positive clones were found through digestion screening/sequencing. false Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni annotation2081800 1 Start range2081800 1 1 3 BBa_K300095_sequence 1 atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggtactagagcactggctgaaggaacccgtatttttgatccggtgacaggcacaactcatcgtatcgaggatgtggttggtgggcgtaaaccgattcatgtggtggcagctgctaaagatggtacactgcatgcacgtccggtagtaagctggttcgatcagggtacacgtgatgtgattggactgcgtattgccggtggcgctattctgtgggctactcctgaccataaagtcctgacggaatatggatggcgtgctgctggtgaactgcgcaaaggtgaccgtgttgctcaacctcgtcgtttcgatggttttggcgattctgccccaattcctgctcgtgttcaggctctggctgatgccctggatgacaaatttctgcacgatatgctggccgaagaactgcgttattcagtgatccgtgaagtcctgcctacacgtcgtgctcgtacatttggtctggaagtggaagagctgcatacactggttgctgagggtgttgtagtacacaattgc BBa_K300002_sequence 1 atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc BBa_K300077_sequence 1 actaga BBa_K300004_sequence 1 gcactggctgaaggaacccgtatttttgatccggtgacaggcacaactcatcgtatcgaggatgtggttggtgggcgtaaaccgattcatgtggtggcagctgctaaagatggtacactgcatgcacgtccggtagtaagctggttcgatcagggtacacgtgatgtgattggactgcgtattgccggtggcgctattctgtgggctactcctgaccataaagtcctgacggaatatggatggcgtgctgctggtgaactgcgcaaaggtgaccgtgttgctcaacctcgtcgtttcgatggttttggcgattctgccccaattcctgctcgtgttcaggctctggctgatgccctggatgacaaatttctgcacgatatgctggccgaagaactgcgttattcagtgatccgtgaagtcctgcctacacgtcgtgctcgtacatttggtctggaagtggaagagctgcatacactggttgctgagggtgttgtagtacacaattgc BBa_K105012_sequence 1 ggtgaaaatttgtattttcaatctggtggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z