BBa_K300096
type: ComponentDefinition
persistent identity: BBa K300096
display id: BBa_K300096
version: 1
was generated by: iGEM to SBOL conversion
description: Double phasin and intein separed by a flexible protein domain linker
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
mutable description: a
discontinued: true
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 4140
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
is replaced by: BBa_K300084
creator: Giacomo Zambianchi
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
component: BBa_K105012
component: BBa_K300077
component: BBa_K300003
component: BBa_K300002
component: BBa_K300004
sequence annotation: BBa_K300002
sequence annotation: BBa_K300077
sequence annotation: BBa_K105012
sequence annotation: BBa_K300003
sequence annotation: BBa_K300004
sequence: https://synbiohub.org/public/igem/BBa_K300096_sequence/1

BBa_K105012
type: Component
persistent identity: component2092419
display id: component2092419
version: 1
top level: BBa_K300096
definition: BBa_K105012
access: public

BBa_K300077
type: Component
persistent identity: component2092418
display id: component2092418
version: 1
top level: BBa_K300096
definition: scar
access: public

BBa_K300003
type: Component
persistent identity: component2092417
display id: component2092417
version: 1
top level: BBa_K300096
definition: BBa_K300003
access: public

BBa_K300077
type: Component
persistent identity: component2092416
display id: component2092416
version: 1
top level: BBa_K300096
definition: scar
access: public

BBa_K105012
type: Component
persistent identity: component2092415
display id: component2092415
version: 1
top level: BBa_K300096
definition: BBa_K105012
access: public

BBa_K300077
type: Component
persistent identity: component2092414
display id: component2092414
version: 1
top level: BBa_K300096
definition: scar
access: public

BBa_K300002
type: Component
persistent identity: component2092413
display id: component2092413
version: 1
top level: BBa_K300096
definition: BBa_K300002
access: public

BBa_K300004
type: Component
persistent identity: component2092421
display id: component2092421
version: 1
top level: BBa_K300096
definition: Intein
access: public

BBa_K300077
type: Component
persistent identity: component2092420
display id: component2092420
version: 1
top level: BBa_K300096
definition: scar
access: public

BBa_K300002
type: SequenceAnnotation
persistent identity: annotation2092413
display id: annotation2092413
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092413/range2092413/1
component: BBa_K300002

https://synbiohub.org/public/igem/BBa_K300096/annotation2092413/range2092413/1
type: Range
persistent identity: range2092413
display id: range2092413
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 1
end: 576
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2092414
display id: annotation2092414
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092414/range2092414/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300096/annotation2092414/range2092414/1
type: Range
persistent identity: range2092414
display id: range2092414
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 577
end: 582
orientation: inline

BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2092415
display id: annotation2092415
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092415/range2092415/1
component: BBa_K105012

https://synbiohub.org/public/igem/BBa_K300096/annotation2092415/range2092415/1
type: Range
persistent identity: range2092415
display id: range2092415
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 583
end: 612
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2092416
display id: annotation2092416
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092416/range2092416/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300096/annotation2092416/range2092416/1
type: Range
persistent identity: range2092416
display id: range2092416
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 613
end: 618
orientation: inline

BBa_K300003
type: SequenceAnnotation
persistent identity: annotation2092417
display id: annotation2092417
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092417/range2092417/1
component: BBa_K300003

https://synbiohub.org/public/igem/BBa_K300096/annotation2092417/range2092417/1
type: Range
persistent identity: range2092417
display id: range2092417
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 619
end: 1194
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2092418
display id: annotation2092418
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092418/range2092418/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300096/annotation2092418/range2092418/1
type: Range
persistent identity: range2092418
display id: range2092418
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 1195
end: 1200
orientation: inline

BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2092419
display id: annotation2092419
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092419/range2092419/1
component: BBa_K105012

https://synbiohub.org/public/igem/BBa_K300096/annotation2092419/range2092419/1
type: Range
persistent identity: range2092419
display id: range2092419
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 1201
end: 1230
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2092420
display id: annotation2092420
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092420/range2092420/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300096/annotation2092420/range2092420/1
type: Range
persistent identity: range2092420
display id: range2092420
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 1231
end: 1236
orientation: inline

BBa_K300004
type: SequenceAnnotation
persistent identity: annotation2092421
display id: annotation2092421
version: 1
top level: BBa_K300096
location: https://synbiohub.org/public/igem/BBa_K300096/annotation2092421/range2092421/1
component: BBa_K300004

https://synbiohub.org/public/igem/BBa_K300096/annotation2092421/range2092421/1
type: Range
persistent identity: range2092421
display id: range2092421
version: 1
top level: BBa_K300096
direction: direction/reverse
start: 1237
end: 1743
orientation: inline

BBa_K300084
type: ComponentDefinition
persistent identity: BBa K300084
display id: BBa_K300084
version: 1
was generated by: iGEM to SBOL conversion
description: Phasins with intein downstream (separated by a flexible protein domain linker)
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
mutable description: Phasins (head and internal domain work as reported in <partinfo>BBa_K300002</partinfo> and <partinfo>BBa_K300003</partinfo>), while intein is described in <partinfo>BBa_K300004</partinfo>. This is a composite part with two phasins (head and internal domain) and an intein separed by a 10aa flexible protein domain linker, which has the function to improve, enhance and ease the folding of these fusion proteins.
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 4140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Giacomo Zambianchi, Sara Bisi, Paolo Magni
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
component: BBa_K300002
component: BBa_K300003
component: BBa_K300077
component: BBa_K105012
component: BBa_K300004
sequence annotation: BBa_K300002
sequence annotation: BBa_K300077
sequence annotation: BBa_K105012
sequence annotation: BBa_K300003
sequence annotation: BBa_K300004
sequence: https://synbiohub.org/public/igem/BBa_K300084_sequence/1

BBa_K300002
type: Component
persistent identity: component2088232
display id: component2088232
version: 1
top level: BBa_K300084
definition: BBa_K300002
access: public

BBa_K300003
type: Component
persistent identity: component2088236
display id: component2088236
version: 1
top level: BBa_K300084
definition: BBa_K300003
access: public

BBa_K300077
type: Component
persistent identity: component2088235
display id: component2088235
version: 1
top level: BBa_K300084
definition: scar
access: public

BBa_K105012
type: Component
persistent identity: component2088234
display id: component2088234
version: 1
top level: BBa_K300084
definition: BBa_K105012
access: public

BBa_K300077
type: Component
persistent identity: component2088233
display id: component2088233
version: 1
top level: BBa_K300084
definition: scar
access: public

BBa_K300004
type: Component
persistent identity: component2088240
display id: component2088240
version: 1
top level: BBa_K300084
definition: Intein
access: public

BBa_K300077
type: Component
persistent identity: component2088239
display id: component2088239
version: 1
top level: BBa_K300084
definition: scar
access: public

BBa_K105012
type: Component
persistent identity: component2088238
display id: component2088238
version: 1
top level: BBa_K300084
definition: BBa_K105012
access: public

BBa_K300077
type: Component
persistent identity: component2088237
display id: component2088237
version: 1
top level: BBa_K300084
definition: scar
access: public

BBa_K300002
type: SequenceAnnotation
persistent identity: annotation2088232
display id: annotation2088232
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088232/range2088232/1
component: BBa_K300002

https://synbiohub.org/public/igem/BBa_K300084/annotation2088232/range2088232/1
type: Range
persistent identity: range2088232
display id: range2088232
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 1
end: 576
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2088233
display id: annotation2088233
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088233/range2088233/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300084/annotation2088233/range2088233/1
type: Range
persistent identity: range2088233
display id: range2088233
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 577
end: 582
orientation: inline

BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2088234
display id: annotation2088234
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088234/range2088234/1
component: BBa_K105012

https://synbiohub.org/public/igem/BBa_K300084/annotation2088234/range2088234/1
type: Range
persistent identity: range2088234
display id: range2088234
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 583
end: 612
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2088235
display id: annotation2088235
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088235/range2088235/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300084/annotation2088235/range2088235/1
type: Range
persistent identity: range2088235
display id: range2088235
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 613
end: 618
orientation: inline

BBa_K300003
type: SequenceAnnotation
persistent identity: annotation2088236
display id: annotation2088236
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088236/range2088236/1
component: BBa_K300003

https://synbiohub.org/public/igem/BBa_K300084/annotation2088236/range2088236/1
type: Range
persistent identity: range2088236
display id: range2088236
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 619
end: 1194
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2088237
display id: annotation2088237
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088237/range2088237/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300084/annotation2088237/range2088237/1
type: Range
persistent identity: range2088237
display id: range2088237
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 1195
end: 1200
orientation: inline

BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2088238
display id: annotation2088238
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088238/range2088238/1
component: BBa_K105012

https://synbiohub.org/public/igem/BBa_K300084/annotation2088238/range2088238/1
type: Range
persistent identity: range2088238
display id: range2088238
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 1201
end: 1230
orientation: inline

BBa_K300077
type: SequenceAnnotation
persistent identity: annotation2088239
display id: annotation2088239
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088239/range2088239/1
component: BBa_K300077

https://synbiohub.org/public/igem/BBa_K300084/annotation2088239/range2088239/1
type: Range
persistent identity: range2088239
display id: range2088239
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 1231
end: 1236
orientation: inline

BBa_K300004
type: SequenceAnnotation
persistent identity: annotation2088240
display id: annotation2088240
version: 1
top level: BBa_K300084
location: https://synbiohub.org/public/igem/BBa_K300084/annotation2088240/range2088240/1
component: BBa_K300004

https://synbiohub.org/public/igem/BBa_K300084/annotation2088240/range2088240/1
type: Range
persistent identity: range2088240
display id: range2088240
version: 1
top level: BBa_K300084
direction: direction/reverse
start: 1237
end: 1743
orientation: inline

BBa_K105012
type: ComponentDefinition
persistent identity: BBa K105012
display id: BBa_K105012
version: 1
was generated by: iGEM to SBOL conversion
description: 10 aa flexible protein domain linker
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered <br\n> The amino acide sequence corresponds to the interdomaine linker of xxx. ''paper''
owned by: james
owned by: myers
mutable description: This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled. <br\n>
discontinued: false
dominant: true
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
star: true
creator: Manuel Gersbacher, Katja Karstens
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_K105012_sequence/1

BBa_K300002
type: ComponentDefinition
persistent identity: BBa K300002
display id: BBa_K300002
version: 1
was generated by: iGEM to SBOL conversion
description: Phasin (PhaP) - head domain
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: Ralstonia eutropha genomic DNA.
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It is identical to <partinfo>BBa_K208001</partinfo>, but it lacks the stop codon in order to support protein fusions. It has been designed as a head domain: The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix) The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix) For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions. CONSTRUCTION METHOD: <partinfo>BBa_K208001</partinfo> (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers: phaP10F 5'-GCTTCTAGATGATCCTCACCCCGGAACA-3' phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3' in order to delete the stop codon. The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with <partinfo>pSB1AK3</partinfo> (previously cut with XbaI-SpeI and dephosphorylated). Positive clones were found through digestion screening/sequencing.
star: false
creator: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K300002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2081800
display id: annotation2081800
version: 1
top level: BBa_K300002
location: https://synbiohub.org/public/igem/BBa_K300002/annotation2081800/range2081800/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K300002/annotation2081800/range2081800/1
type: Range
persistent identity: range2081800
display id: range2081800
version: 1
top level: BBa_K300002
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K300003
type: ComponentDefinition
persistent identity: BBa K300003
display id: BBa_K300003
version: 1
was generated by: iGEM to SBOL conversion
description: Phasin (PhaP) - internal domain
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: It is identical to <partinfo>BBa_K208001</partinfo>, but it lacks the stop codon in order to support protein fusions. It has been designed as a head domain: The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix) The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix) For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions. '''CONSTRUCTION METHOD:''' <partinfo>BBa_K208001</partinfo> (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers: phaPSF: 5'GCTTCTAGAATGATCCTCACCCCGGAACA-3' phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3' in order to delete the stop codon. The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with <partinfo>pSB1A3</partinfo> (previously cut with XbaI-SpeI and dephosphorylated). Positive clones were found through digestion screening/sequencing.
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Ralstonia eutropha genomic DNA.
star: false
creator: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K300003_sequence/1

Intein
type: ComponentDefinition
persistent identity: BBa K300004
display id: BBa_K300004
version: 1
was generated by: iGEM to SBOL conversion
description: Engineered pH-inducible intein (codon optimized for E. coli) - internal domain
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: The original sequence comes from M. tuberculosis genomic DNA (RecA gene), but it has been highly engineered (according to [1]) to create a pH-inducible protein domain.
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Codon optimized for E. coli. The amino acid sequence comes from [1].
star: false
creator: Sara Bisi, Paolo Magni
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K300004_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa K300077
display id: BBa_K300077
version: 1
was generated by: iGEM to SBOL conversion
description: RFC23 scar sequence
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:11:51Z
owned by: james
owned by: myers
mutable description: RFC23 scar sequence (Silver-Silver mixed site)
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 4140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Giacomo Zambianchi, Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300077_sequence/1

https://synbiohub.org/public/igem/BBa_K300096_sequence/1
type: Sequence
persistent identity: BBa K300096 sequence
display id: BBa_K300096_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggtactagaatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggtactagagcactggctgaaggaacccgtatttttgatccggtgacaggcacaactcatcgtatcgaggatgtggttggtgggcgtaaaccgattcatgtggtggcagctgctaaagatggtacactgcatgcacgtccggtagtaagctggttcgatcagggtacacgtgatgtgattggactgcgtattgccggtggcgctattctgtgggctactcctgaccataaagtcctgacggaatatggatggcgtgctgctggtgaactgcgcaaaggtgaccgtgttgctcaacctcgtcgtttcgatggttttggcgattctgccccaattcctgctcgtgttcaggctctggctgatgccctggatgacaaatttctgcacgatatgctggccgaagaactgcgttattcagtgatccgtgaagtcctgcctacacgtcgtgctcgtacatttggtctggaagtggaagagctgcatacactggttgctgagggtgttgtagtacacaattgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300084_sequence/1
type: Sequence
persistent identity: BBa K300084 sequence
display id: BBa_K300084_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggtactagaatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagaggtgaaaatttgtattttcaatctggtggtactagagcactggctgaaggaacccgtatttttgatccggtgacaggcacaactcatcgtatcgaggatgtggttggtgggcgtaaaccgattcatgtggtggcagctgctaaagatggtacactgcatgcacgtccggtagtaagctggttcgatcagggtacacgtgatgtgattggactgcgtattgccggtggcgctattctgtgggctactcctgaccataaagtcctgacggaatatggatggcgtgctgctggtgaactgcgcaaaggtgaccgtgttgctcaacctcgtcgtttcgatggttttggcgattctgccccaattcctgctcgtgttcaggctctggctgatgccctggatgacaaatttctgcacgatatgctggccgaagaactgcgttattcagtgatccgtgaagtcctgcctacacgtcgtgctcgtacatttggtctggaagtggaagagctgcatacactggttgctgagggtgttgtagtacacaattgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105012_sequence/1
type: Sequence
persistent identity: BBa K105012 sequence
display id: BBa_K105012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgaaaatttgtattttcaatctggtggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300002_sequence/1
type: Sequence
persistent identity: BBa K300002 sequence
display id: BBa_K300002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300003_sequence/1
type: Sequence
persistent identity: BBa K300003 sequence
display id: BBa_K300003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300004_sequence/1
type: Sequence
persistent identity: BBa K300004 sequence
display id: BBa_K300004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactggctgaaggaacccgtatttttgatccggtgacaggcacaactcatcgtatcgaggatgtggttggtgggcgtaaaccgattcatgtggtggcagctgctaaagatggtacactgcatgcacgtccggtagtaagctggttcgatcagggtacacgtgatgtgattggactgcgtattgccggtggcgctattctgtgggctactcctgaccataaagtcctgacggaatatggatggcgtgctgctggtgaactgcgcaaaggtgaccgtgttgctcaacctcgtcgtttcgatggttttggcgattctgccccaattcctgctcgtgttcaggctctggctgatgccctggatgacaaatttctgcacgatatgctggccgaagaactgcgttattcagtgatccgtgaagtcctgcctacacgtcgtgctcgtacatttggtctggaagtggaagagctgcatacactggttgctgagggtgttgtagtacacaattgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300077_sequence/1
type: Sequence
persistent identity: BBa K300077 sequence
display id: BBa_K300077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z