LOCUS       BBa_K300098             2672 bp    DNA     linear       20-Nov-2024
DEFINITION  BBa_K300099 construction intermediate
ACCESSION   BBa_K300098
VERSION     BBa_K300098.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     misc_feature    1..576
                     /label=BBa_K300002
     misc_feature    577..582
                     /label=BBa_K300077
     misc_feature    583..612
                     /label=BBa_K105012
     misc_feature    613..618
                     /label=BBa_K300077
     misc_feature    619..1194
                     /label=BBa_K300003
     misc_feature    1195..1200
                     /label=BBa_K300077
     misc_feature    1201..1230
                     /label=BBa_K105012
     misc_feature    1231..1236
                     /label=BBa_K300077
     misc_feature    1237..1812
                     /label=BBa_K300003
     misc_feature    1..1812
                     /label=BBa_K300097
     misc_feature    1813..1818
                     /label=BBa_K300077
     CDS             1819..2536
                     /label=GFP fusion brick
     misc_feature    1819..1819
                     /label=deletion of TG
     CDS             1819..2536
                     /label=BBa_K125500
     misc_feature    2537..2543
                     /label=BBa_K300076
     stem_loop       2555..2598
                     /label=stem_loop
     misc_feature    2544..2623
                     /label=BBa_B0010
     terminator      2544..2623
                     /label=BBa_B0010
     stem_loop       2639..2658
                     /label=T7 TE
     misc_feature    2665..2665
                     /label=stop
     polyA_site      2659..2672
                     /label=polya
     misc_feature    2632..2672
                     /label=BBa_B0012
     terminator      2632..2672
                     /label=BBa_B0012
     terminator      2544..2672
                     /label=BBa_B0015
     misc_feature    1819..2672
                     /label=BBa_K300005
ORIGIN
        1 atgatcctca ccccggaaca agttgcagca gcgcaaaagg ccaacctcga aacgctgttc
       61 ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg tcgagctgaa ccttcaggtc
      121 gtcaagactt cgttcgcaga aggcgttgac aacgccaaga aggcgctgtc ggccaaggac
      181 gcacaggaac tgctggccat ccaggccgca gccgtgcagc cggttgccga aaagaccctg
      241 gcctacaccc gccacctgta tgaaatcgct tcggaaaccc agagcgagtt caccaaggta
      301 gccgaggctc aactggccga aggctcgaag aacgtgcaag cgctggtcga gaacctcgcc
      361 aagaacgccc cggccggttc ggaatcgacc gtggccatcg tgaagtcggc gatctccgct
      421 gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc aagcggtcga aatcgctgaa
      481 accaacttcc aggctgcggc tacggctgcc accaaggctg cccagcaagc cagcgccacg
      541 gcccgtacgg ccacggcaaa gaagacgacg gctgccacta gaggtgaaaa tttgtatttt
      601 caatctggtg gtactagaat gatcctcacc ccggaacaag ttgcagcagc gcaaaaggcc
      661 aacctcgaaa cgctgttcgg cctgaccacc aaggcgtttg aaggcgtcga aaagctcgtc
      721 gagctgaacc ttcaggtcgt caagacttcg ttcgcagaag gcgttgacaa cgccaagaag
      781 gcgctgtcgg ccaaggacgc acaggaactg ctggccatcc aggccgcagc cgtgcagccg
      841 gttgccgaaa agaccctggc ctacacccgc cacctgtatg aaatcgcttc ggaaacccag
      901 agcgagttca ccaaggtagc cgaggctcaa ctggccgaag gctcgaagaa cgtgcaagcg
      961 ctggtcgaga acctcgccaa gaacgccccg gccggttcgg aatcgaccgt ggccatcgtg
     1021 aagtcggcga tctccgctgc caacaacgcc tacgagtcgg tgcagaaggc gaccaagcaa
     1081 gcggtcgaaa tcgctgaaac caacttccag gctgcggcta cggctgccac caaggctgcc
     1141 cagcaagcca gcgccacggc ccgtacggcc acggcaaaga agacgacggc tgccactaga
     1201 ggtgaaaatt tgtattttca atctggtggt actagaatga tcctcacccc ggaacaagtt
     1261 gcagcagcgc aaaaggccaa cctcgaaacg ctgttcggcc tgaccaccaa ggcgtttgaa
     1321 ggcgtcgaaa agctcgtcga gctgaacctt caggtcgtca agacttcgtt cgcagaaggc
     1381 gttgacaacg ccaagaaggc gctgtcggcc aaggacgcac aggaactgct ggccatccag
     1441 gccgcagccg tgcagccggt tgccgaaaag accctggcct acacccgcca cctgtatgaa
     1501 atcgcttcgg aaacccagag cgagttcacc aaggtagccg aggctcaact ggccgaaggc
     1561 tcgaagaacg tgcaagcgct ggtcgagaac ctcgccaaga acgccccggc cggttcggaa
     1621 tcgaccgtgg ccatcgtgaa gtcggcgatc tccgctgcca acaacgccta cgagtcggtg
     1681 cagaaggcga ccaagcaagc ggtcgaaatc gctgaaacca acttccaggc tgcggctacg
     1741 gctgccacca aggctgccca gcaagccagc gccacggccc gtacggccac ggcaaagaag
     1801 acgacggctg ccactagacg taaaggagaa gaacttttca ctggagttgt cccaattctt
     1861 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     1921 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1981 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     2041 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     2101 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     2161 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     2221 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     2281 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     2341 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2401 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     2461 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2521 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2581 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2641 ctcaccttcg ggtgggcctt tctgcgttta ta
//