LOCUS       BBa_K300099             2752 bp    DNA     linear       20-Nov-2024
DEFINITION  Measurement system for BBa_K300097
ACCESSION   BBa_K300099
VERSION     BBa_K300099.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=Start
     misc_feature    81..656
                     /label=BBa_K300002
     misc_feature    657..662
                     /label=BBa_K300077
     misc_feature    663..692
                     /label=BBa_K105012
     misc_feature    693..698
                     /label=BBa_K300077
     misc_feature    699..1274
                     /label=BBa_K300003
     misc_feature    1275..1280
                     /label=BBa_K300077
     misc_feature    1281..1310
                     /label=BBa_K105012
     misc_feature    1311..1316
                     /label=BBa_K300077
     misc_feature    1317..1892
                     /label=BBa_K300003
     misc_feature    81..1892
                     /label=BBa_K300097
     misc_feature    1893..1898
                     /label=BBa_K300077
     CDS             1899..2616
                     /label=GFP fusion brick
     misc_feature    1899..1899
                     /label=deletion of TG
     CDS             1899..2616
                     /label=BBa_K125500
     misc_feature    2617..2623
                     /label=BBa_K300076
     stem_loop       2635..2678
                     /label=stem_loop
     misc_feature    2624..2703
                     /label=BBa_B0010
     terminator      2624..2703
                     /label=BBa_B0010
     stem_loop       2719..2738
                     /label=T7 TE
     misc_feature    2745..2745
                     /label=stop
     polyA_site      2739..2752
                     /label=polya
     misc_feature    2712..2752
                     /label=BBa_B0012
     terminator      2712..2752
                     /label=BBa_B0012
     terminator      2624..2752
                     /label=BBa_B0015
     misc_feature    1899..2752
                     /label=BBa_K300005
     misc_feature    81..2752
                     /label=BBa_K300098
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      121 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      181 tcgagctgaa ccttcaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga
      241 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc
      301 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc
      361 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag
      421 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg
      481 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc
      541 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg
      601 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgccacta
      661 gaggtgaaaa tttgtatttt caatctggtg gtactagaat gatcctcacc ccggaacaag
      721 ttgcagcagc gcaaaaggcc aacctcgaaa cgctgttcgg cctgaccacc aaggcgtttg
      781 aaggcgtcga aaagctcgtc gagctgaacc ttcaggtcgt caagacttcg ttcgcagaag
      841 gcgttgacaa cgccaagaag gcgctgtcgg ccaaggacgc acaggaactg ctggccatcc
      901 aggccgcagc cgtgcagccg gttgccgaaa agaccctggc ctacacccgc cacctgtatg
      961 aaatcgcttc ggaaacccag agcgagttca ccaaggtagc cgaggctcaa ctggccgaag
     1021 gctcgaagaa cgtgcaagcg ctggtcgaga acctcgccaa gaacgccccg gccggttcgg
     1081 aatcgaccgt ggccatcgtg aagtcggcga tctccgctgc caacaacgcc tacgagtcgg
     1141 tgcagaaggc gaccaagcaa gcggtcgaaa tcgctgaaac caacttccag gctgcggcta
     1201 cggctgccac caaggctgcc cagcaagcca gcgccacggc ccgtacggcc acggcaaaga
     1261 agacgacggc tgccactaga ggtgaaaatt tgtattttca atctggtggt actagaatga
     1321 tcctcacccc ggaacaagtt gcagcagcgc aaaaggccaa cctcgaaacg ctgttcggcc
     1381 tgaccaccaa ggcgtttgaa ggcgtcgaaa agctcgtcga gctgaacctt caggtcgtca
     1441 agacttcgtt cgcagaaggc gttgacaacg ccaagaaggc gctgtcggcc aaggacgcac
     1501 aggaactgct ggccatccag gccgcagccg tgcagccggt tgccgaaaag accctggcct
     1561 acacccgcca cctgtatgaa atcgcttcgg aaacccagag cgagttcacc aaggtagccg
     1621 aggctcaact ggccgaaggc tcgaagaacg tgcaagcgct ggtcgagaac ctcgccaaga
     1681 acgccccggc cggttcggaa tcgaccgtgg ccatcgtgaa gtcggcgatc tccgctgcca
     1741 acaacgccta cgagtcggtg cagaaggcga ccaagcaagc ggtcgaaatc gctgaaacca
     1801 acttccaggc tgcggctacg gctgccacca aggctgccca gcaagccagc gccacggccc
     1861 gtacggccac ggcaaagaag acgacggctg ccactagacg taaaggagaa gaacttttca
     1921 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1981 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     2041 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     2101 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     2161 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     2221 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     2281 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     2341 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     2401 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     2461 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     2521 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     2581 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     2641 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2701 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//