LOCUS       BBa_K300980             1707 bp    DNA     linear       15-Jun-2024
DEFINITION  BBa_K300001 construction intermediate (provided by Mr Gene)
ACCESSION   BBa_K300980
VERSION     BBa_K300980.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=MfeI site
     misc_feature    1..6
                     /label=BBa_B0047
     misc_feature    7..14
                     /label=BBa_K300990
     misc_feature    15..20
                     /label=AvrII site
     misc_feature    15..20
                     /label=BBa_B0048
     misc_feature    21..65
                     /label=BBa_K300986
     misc_feature    66..71
                     /label=AvrII site
     misc_feature    66..71
                     /label=BBa_B0048
     misc_feature    72..77
                     /label=BBa_M31983
     RBS             78..88
                     /label=RBS-4\Weaker
     RBS             84..87
                     /label=RBS
     misc_feature    78..88
                     /label=BBa_B0033
     RBS             78..88
                     /label=BBa_B0033
     misc_feature    90..95
                     /label=BBa_M31992
     misc_feature    96..129
                     /label=BBa_J61046 (loxP)
     promoter        130..527
                     /label=TEF2 (A. gossypii)+RBS
     CDS             528..1337
                     /label=kanR(Tn903)
     misc_feature    803..803
                     /label=A->T
     misc_feature    1070..1070
                     /label=T->C
     misc_feature    1338..1602
                     /label=TEF2 terminator (A. gossypii)
     misc_feature    1603..1636
                     /label=BBa_J61046 (loxP)
     misc_feature    130..1602
                     /label=kanMX cassette
     misc_feature    96..1636
                     /label=BBa_K300989
     misc_feature    1637..1642
                     /label=NheI site
     misc_feature    1637..1642
                     /label=BBa_B0045
     misc_feature    1643..1687
                     /label=BBa_K300987
     misc_feature    1688..1693
                     /label=NheI site
     misc_feature    1688..1693
                     /label=BBa_B0045
     misc_feature    1694..1701
                     /label=BBa_K300990
     misc_feature    1702..1707
                     /label=NsiI site
     misc_feature    1702..1707
                     /label=BBa_B0046
ORIGIN
        1 caattgcctg caggcctagg gtgtgaacca atgtatccag caccacctgt aaccaaaaca
       61 attttcctag ggaattctca cacaggacta ctagtataac ttcgtataat gtatgctata
      121 cgaagttatt aggtctagag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
      181 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
      241 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
      301 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc
      361 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
      421 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
      481 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaggaaa
      541 agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
      601 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
      661 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
      721 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
      781 ttatccgtac tcctgatgat gcttggttac tcaccactgc gatccccggc aaaacagcat
      841 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
      901 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
      961 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     1021 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcac aagcttttgc
     1081 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     1141 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     1201 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     1261 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     1321 tcgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca agaacttgtc
     1381 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
     1441 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
     1501 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
     1561 ccgccatcca gtgtcgaaaa cgagctctcg agaaccctta atataacttc gtataatgta
     1621 tgctatacga agttatgcta gccctgagtt caattctagc gcaaaggaat taccaagacc
     1681 attggccgct agccctgcag gatgcat
//