BBa_B0046
1
NsiI
NsiI restriction enzyme site (PstI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
NsiI recognition site. Digestion with NsiI generates a compatible cohesive end to PstI.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822730
1
NsiI site
range1822730
1
1
6
BBa_B0047
1
MfeI
MfeI restriction enzyme site (EcoRI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
MfeI recognition site. Digestion with MfeI yields a compatible cohesive end to EcoRI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822740
1
MfeI site
range1822740
1
1
6
BBa_M31992
1
SpeI
SpeI restriction enzyme site
2007-02-28T12:00:00Z
2015-05-08T01:14:01Z
none
SpeI restriction enzyme site
false
false
_102_
0
1373
102
Not in stock
false
none
false
Tiffany Guo
BBa_K300990
1
SbfI
SbfI restriction site
2010-10-01T11:00:00Z
2015-05-08T01:11:52Z
-
This site can also be cut with PstI restriction enzyme.
false
false
_420_
0
2621
9
Not in stock
false
Restriction site sequence
false
Lorenzo Pasotti, Paolo Magni
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7028
1
BBa_B0033
range7028
1
1
11
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation1714
1
RBS
range1714
1
7
10
BBa_M31983
1
EcoRI
EcoRI restriction enzyme site
2007-02-28T12:00:00Z
2015-05-08T01:14:01Z
none
EcoRI restriction enzyme site
false
true
_102_
0
1373
102
Not in stock
false
none
false
Tiffany Guo
BBa_K300980
1
BBa_K300980
BBa_K300001 construction intermediate (provided by Mr Gene)
2010-10-08T11:00:00Z
2015-05-08T01:11:51Z
Mr Gene synthesis service (www.mrgene.com).
This part has been provided by Mr Gene synthesis service and it has been used to construct BBa_K300001 plasmid.
false
false
_420_
0
2621
9
Not in stock
false
See <partinfo>BBa_K300001</partinfo>.
false
Susanna Zucca, Federica Sampietro, Paolo Magni
component2084604
1
BBa_K300987
component2084590
1
BBa_B0033
component2084585
1
BBa_K300986
component2084603
1
BBa_B0045
component2084588
1
BBa_M31983
component2084606
1
BBa_B0045
component2084607
1
BBa_K300990
component2084601
1
BBa_K300989
component2084609
1
BBa_B0046
component2084581
1
BBa_B0047
component2084592
1
BBa_M31992
component2084587
1
BBa_B0048
component2084584
1
BBa_B0048
component2084582
1
BBa_K300990
annotation2084601
1
BBa_K300989
range2084601
1
96
1636
annotation2084604
1
BBa_K300987
range2084604
1
1643
1687
annotation2084590
1
BBa_B0033
range2084590
1
78
88
annotation2084607
1
BBa_K300990
range2084607
1
1694
1701
annotation2084603
1
BBa_B0045
range2084603
1
1637
1642
annotation2084592
1
BBa_M31992
range2084592
1
90
95
annotation2084581
1
BBa_B0047
range2084581
1
1
6
annotation2084584
1
BBa_B0048
range2084584
1
15
20
annotation2084585
1
BBa_K300986
range2084585
1
21
65
annotation2084582
1
BBa_K300990
range2084582
1
7
14
annotation2084606
1
BBa_B0045
range2084606
1
1688
1693
annotation2084588
1
BBa_M31983
range2084588
1
72
77
annotation2084587
1
BBa_B0048
range2084587
1
66
71
annotation2084609
1
BBa_B0046
range2084609
1
1702
1707
BBa_B0045
1
NheI
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822724
1
NheI site
range1822724
1
1
6
BBa_K300987
1
HR2-GAL10
Homologous region in S288C yeast genome (GAL10 ORF)
2010-10-02T11:00:00Z
2015-05-08T01:11:52Z
-
-
false
false
_420_
0
2621
9
Not in stock
true
-
false
Susanna Zucca, Federica Sampietro, Paolo Magni
BBa_K300986
1
HR1-GAL1
Homologous region in S288C yeast genome (GAL1 ORF)
2010-10-02T11:00:00Z
2015-05-08T01:11:52Z
-
-
false
false
_420_
0
2621
9
Not in stock
true
-
false
Susanna Zucca, Federica Sampietro, Paolo Magni
BBa_B0048
1
AvrII
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822744
1
AvrII site
range1822744
1
1
6
BBa_K300989
1
KanMX(lox)
[LoxP-KanMX-LoxP] excisable marker for yeast
2010-10-02T11:00:00Z
2015-05-08T01:11:52Z
pUG6 vector (GenBank: AF298793.1).
This cassette is to be used in yeast.
It is composed by the kanMX gene from the E. coli transposon Tn903 that confers the resistance to the aminoglycoside antibiotic G418 to yeast (commonly used at 200 ug/ml working concentration). This gene is expressed by the TEF2 promoter/RBS and terminated by the TEF2 transcriptional terminator from Ashbya gossypii filamentous fungus.
The cassette is flanked by two loxP sites and for this reason it can be excised with Cre/loxP mediated marker removal recombination procedure.
ADVANTAGES IN GENOME ENGINEERING APPLICATIONS:
This part can be used to select positive integrants in the yeast genome and then it can be eliminated through Cre/loxP procedure. This enables the engineering of yeast genome in multiple steps, always using the same dominant selection marker.
false
false
_420_
0
2621
9
Not in stock
true
The original sequence of loxP-KanMX-loxP from pUG6 vector has two NsiI restriction sites in kanR(Tn903) coding sequence and they have been removed in this part by changing codons.
The TEF2 promoter has a XbaI and a PstI restriction sites, which have not been removed to avoid the change of the promoter strength. For this reason, this part is not fully compatible with RFC10 BioBrick Standard Assembly, but it can be assembled by using EcoRI-SpeI enzymes.
false
Lorenzo Pasotti, Giacomo Zambianchi, Federica Sampietro, Paolo Magni
annotation2081360
1
BBa_J61046 (loxP)
range2081360
1
1
34
annotation2081362
1
kanR(Tn903)
range2081362
1
433
1242
annotation2081364
1
T->C
range2081364
1
975
975
annotation2081365
1
TEF2 terminator (A. gossypii)
range2081365
1
1243
1507
annotation2081366
1
BBa_J61046 (loxP)
range2081366
1
1508
1541
annotation2081363
1
A->T
range2081363
1
708
708
annotation2081367
1
kanMX cassette
range2081367
1
35
1507
annotation2081361
1
TEF2 (A. gossypii)+RBS
range2081361
1
35
432
BBa_B0048_sequence
1
cctagg
BBa_B0033_sequence
1
tcacacaggac
BBa_B0046_sequence
1
atgcat
BBa_K300989_sequence
1
ataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttat
BBa_B0047_sequence
1
caattg
BBa_B0045_sequence
1
gctagc
BBa_K300986_sequence
1
gtgtgaaccaatgtatccagcaccacctgtaaccaaaacaatttt
BBa_K300990_sequence
1
cctgcagg
BBa_M31983_sequence
1
gaattc
BBa_K300980_sequence
1
caattgcctgcaggcctagggtgtgaaccaatgtatccagcaccacctgtaaccaaaacaattttcctagggaattctcacacaggactactagtataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttatgctagccctgagttcaattctagcgcaaaggaattaccaagaccattggccgctagccctgcaggatgcat
BBa_M31992_sequence
1
actagt
BBa_K300987_sequence
1
cctgagttcaattctagcgcaaaggaattaccaagaccattggcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z