##gff-version 3 ##sequence-region BBa_K300980 1 1707 BBa_K300980 . sequence_feature 66 71 . + 0 ID=BBa_B0048;Name=BBa_B0048 BBa_K300980 . sequence_feature 66 71 . + 0 ID=annotation1822744;Name=AvrII site;Parent=BBa_B0048 BBa_K300980 . sequence_feature 1688 1693 . + 0 ID=BBa_B0045;Name=BBa_B0045 BBa_K300980 . sequence_feature 1688 1693 . + 0 ID=annotation1822724;Name=NheI site;Parent=BBa_B0045 BBa_K300980 . sequence_feature 72 77 . + 0 ID=BBa_M31983;Name=BBa_M31983 BBa_K300980 . sequence_feature 15 20 . + 0 ID=BBa_B0048;Name=BBa_B0048 BBa_K300980 . sequence_feature 15 20 . + 0 ID=annotation1822744;Name=AvrII site;Parent=BBa_B0048 BBa_K300980 . engineered_region 96 1636 . + 0 ID=BBa_K300989;Name=BBa_K300989 BBa_K300980 . point_mutation 1070 1070 . + 0 ID=annotation2081364;Name=T->C;Parent=BBa_K300989 BBa_K300980 . engineered_region 96 129 . + 0 ID=annotation2081360;Name=BBa_J61046 (loxP);Parent=BBa_K300989 BBa_K300980 . sequence_feature 130 1602 . + 0 ID=annotation2081367;Name=kanMX cassette;Parent=BBa_K300989 BBa_K300980 . sequence_feature 1338 1602 . + 0 ID=annotation2081365;Name=TEF2 terminator (A. gossypii);Parent=BBa_K300989 BBa_K300980 . CDS 528 1337 . + 0 ID=annotation2081362;Name=kanR(Tn903);Parent=BBa_K300989 BBa_K300980 . promoter 130 527 . + 0 ID=annotation2081361;Name=TEF2 (A. gossypii)+RBS;Parent=BBa_K300989 BBa_K300980 . point_mutation 803 803 . + 0 ID=annotation2081363;Name=A->T;Parent=BBa_K300989 BBa_K300980 . engineered_region 1603 1636 . + 0 ID=annotation2081366;Name=BBa_J61046 (loxP);Parent=BBa_K300989 BBa_K300980 . sequence_feature 1694 1701 . + 0 ID=BBa_K300990;Name=BBa_K300990 BBa_K300980 . sequence_feature 1643 1687 . + 0 ID=BBa_K300987;Name=BBa_K300987 BBa_K300980 . sequence_feature 1637 1642 . + 0 ID=BBa_B0045;Name=BBa_B0045 BBa_K300980 . sequence_feature 1637 1642 . + 0 ID=annotation1822724;Name=NheI site;Parent=BBa_B0045 BBa_K300980 . ribosome_entry_site 78 88 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_K300980 . ribosome_entry_site 78 88 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_K300980 . engineered_region 78 88 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_K300980 . ribosome_entry_site 84 87 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_K300980 . sequence_feature 1702 1707 . + 0 ID=BBa_B0046;Name=BBa_B0046 BBa_K300980 . sequence_feature 1702 1707 . + 0 ID=annotation1822730;Name=NsiI site;Parent=BBa_B0046 BBa_K300980 . sequence_feature 7 14 . + 0 ID=BBa_K300990;Name=BBa_K300990 BBa_K300980 . sequence_feature 21 65 . + 0 ID=BBa_K300986;Name=BBa_K300986 BBa_K300980 . sequence_feature 1 6 . + 0 ID=BBa_B0047;Name=BBa_B0047 BBa_K300980 . sequence_feature 1 6 . + 0 ID=annotation1822740;Name=MfeI site;Parent=BBa_B0047 BBa_K300980 . sequence_feature 90 95 . + 0 ID=BBa_M31992;Name=BBa_M31992 >BBa_K300980 caattgcctgcaggcctagggtgtgaaccaatgtatccagcaccacctgtaaccaaaacaattttcctagggaattctc acacaggactactagtataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctc gtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggg gcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggcc gcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaa ttgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttcct tttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttc gaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggt gcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatg atgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtac tcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattca ggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaaca gcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacga gcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcact catggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaa tcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttt tcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagta ctgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatc aaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaata tcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaa cgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttatgctagccctgagttcaattctag cgcaaaggaattaccaagaccattggccgctagccctgcaggatgcat