BBa_K300980
type: ComponentDefinition
persistent identity: BBa K300980
display id: BBa_K300980
version: 1
was generated by: iGEM to SBOL conversion
description: BBa_K300001 construction intermediate (provided by Mr Gene)
created: 2010-10-08T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: Mr Gene synthesis service (www.mrgene.com).
owned by: james
owned by: myers
mutable description: This part has been provided by Mr Gene synthesis service and it has been used to construct BBa_K300001 plasmid.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300001</partinfo>.
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0048
component: BBa_M31983
component: BBa_K300990
component: BBa_B0045
component: BBa_B0047
component: BBa_K300987
component: BBa_B0046
component: BBa_M31992
component: BBa_B0033
component: BBa_K300989
component: BBa_K300986
sequence annotation: BBa_B0047
sequence annotation: BBa_K300990
sequence annotation: BBa_B0048
sequence annotation: BBa_K300986
sequence annotation: BBa_M31983
sequence annotation: BBa_B0033
sequence annotation: BBa_M31992
sequence annotation: BBa_K300989
sequence annotation: BBa_B0045
sequence annotation: BBa_K300987
sequence annotation: BBa_B0046
sequence: https://synbiohub.org/public/igem/BBa_K300980_sequence/1

BBa_B0048
type: Component
persistent identity: component2084584
display id: component2084584
version: 1
top level: BBa_K300980
definition: AvrII
access: public

BBa_M31983
type: Component
persistent identity: component2084588
display id: component2084588
version: 1
top level: BBa_K300980
definition: EcoRI
access: public

BBa_B0048
type: Component
persistent identity: component2084587
display id: component2084587
version: 1
top level: BBa_K300980
definition: AvrII
access: public

BBa_K300990
type: Component
persistent identity: component2084582
display id: component2084582
version: 1
top level: BBa_K300980
definition: SbfI
access: public

BBa_B0045
type: Component
persistent identity: component2084606
display id: component2084606
version: 1
top level: BBa_K300980
definition: NheI
access: public

BBa_B0047
type: Component
persistent identity: component2084581
display id: component2084581
version: 1
top level: BBa_K300980
definition: MfeI
access: public

BBa_K300987
type: Component
persistent identity: component2084604
display id: component2084604
version: 1
top level: BBa_K300980
definition: HR2-GAL10
access: public

BBa_B0046
type: Component
persistent identity: component2084609
display id: component2084609
version: 1
top level: BBa_K300980
definition: NsiI
access: public

BBa_K300990
type: Component
persistent identity: component2084607
display id: component2084607
version: 1
top level: BBa_K300980
definition: SbfI
access: public

BBa_M31992
type: Component
persistent identity: component2084592
display id: component2084592
version: 1
top level: BBa_K300980
definition: SpeI
access: public

BBa_B0033
type: Component
persistent identity: component2084590
display id: component2084590
version: 1
top level: BBa_K300980
definition: BBa_B0033
access: public

BBa_B0045
type: Component
persistent identity: component2084603
display id: component2084603
version: 1
top level: BBa_K300980
definition: NheI
access: public

BBa_K300989
type: Component
persistent identity: component2084601
display id: component2084601
version: 1
top level: BBa_K300980
definition: KanMX(lox)
access: public

BBa_K300986
type: Component
persistent identity: component2084585
display id: component2084585
version: 1
top level: BBa_K300980
definition: HR1-GAL1
access: public

BBa_B0047
type: SequenceAnnotation
persistent identity: annotation2084581
display id: annotation2084581
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084581/range2084581/1
component: BBa_B0047

https://synbiohub.org/public/igem/BBa_K300980/annotation2084581/range2084581/1
type: Range
persistent identity: range2084581
display id: range2084581
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 1
end: 6
orientation: inline

BBa_K300990
type: SequenceAnnotation
persistent identity: annotation2084582
display id: annotation2084582
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084582/range2084582/1
component: BBa_K300990

https://synbiohub.org/public/igem/BBa_K300980/annotation2084582/range2084582/1
type: Range
persistent identity: range2084582
display id: range2084582
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 7
end: 14
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2084584
display id: annotation2084584
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084584/range2084584/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300980/annotation2084584/range2084584/1
type: Range
persistent identity: range2084584
display id: range2084584
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 15
end: 20
orientation: inline

BBa_K300986
type: SequenceAnnotation
persistent identity: annotation2084585
display id: annotation2084585
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084585/range2084585/1
component: BBa_K300986

https://synbiohub.org/public/igem/BBa_K300980/annotation2084585/range2084585/1
type: Range
persistent identity: range2084585
display id: range2084585
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 21
end: 65
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2084587
display id: annotation2084587
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084587/range2084587/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300980/annotation2084587/range2084587/1
type: Range
persistent identity: range2084587
display id: range2084587
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 66
end: 71
orientation: inline

BBa_M31983
type: SequenceAnnotation
persistent identity: annotation2084588
display id: annotation2084588
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084588/range2084588/1
component: BBa_M31983

https://synbiohub.org/public/igem/BBa_K300980/annotation2084588/range2084588/1
type: Range
persistent identity: range2084588
display id: range2084588
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 72
end: 77
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2084590
display id: annotation2084590
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084590/range2084590/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K300980/annotation2084590/range2084590/1
type: Range
persistent identity: range2084590
display id: range2084590
version: 1
top level: BBa_K300980
direction: direction/forward
start: 78
end: 88
orientation: inline

BBa_M31992
type: SequenceAnnotation
persistent identity: annotation2084592
display id: annotation2084592
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084592/range2084592/1
component: BBa_M31992

https://synbiohub.org/public/igem/BBa_K300980/annotation2084592/range2084592/1
type: Range
persistent identity: range2084592
display id: range2084592
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 90
end: 95
orientation: inline

BBa_K300989
type: SequenceAnnotation
persistent identity: annotation2084601
display id: annotation2084601
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084601/range2084601/1
component: BBa_K300989

https://synbiohub.org/public/igem/BBa_K300980/annotation2084601/range2084601/1
type: Range
persistent identity: range2084601
display id: range2084601
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 96
end: 1636
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2084603
display id: annotation2084603
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084603/range2084603/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300980/annotation2084603/range2084603/1
type: Range
persistent identity: range2084603
display id: range2084603
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 1637
end: 1642
orientation: inline

BBa_K300987
type: SequenceAnnotation
persistent identity: annotation2084604
display id: annotation2084604
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084604/range2084604/1
component: BBa_K300987

https://synbiohub.org/public/igem/BBa_K300980/annotation2084604/range2084604/1
type: Range
persistent identity: range2084604
display id: range2084604
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 1643
end: 1687
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2084606
display id: annotation2084606
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084606/range2084606/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300980/annotation2084606/range2084606/1
type: Range
persistent identity: range2084606
display id: range2084606
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 1688
end: 1693
orientation: inline

BBa_K300990
type: SequenceAnnotation
persistent identity: annotation2084607
display id: annotation2084607
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084607/range2084607/1
component: BBa_K300990

https://synbiohub.org/public/igem/BBa_K300980/annotation2084607/range2084607/1
type: Range
persistent identity: range2084607
display id: range2084607
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 1694
end: 1701
orientation: inline

BBa_B0046
type: SequenceAnnotation
persistent identity: annotation2084609
display id: annotation2084609
version: 1
top level: BBa_K300980
location: https://synbiohub.org/public/igem/BBa_K300980/annotation2084609/range2084609/1
component: BBa_B0046

https://synbiohub.org/public/igem/BBa_K300980/annotation2084609/range2084609/1
type: Range
persistent identity: range2084609
display id: range2084609
version: 1
top level: BBa_K300980
direction: direction/reverse
start: 1702
end: 1707
orientation: inline

SbfI
type: ComponentDefinition
persistent identity: BBa K300990
display id: BBa_K300990
version: 1
was generated by: iGEM to SBOL conversion
description: SbfI restriction site
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This site can also be cut with PstI restriction enzyme.
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Restriction site sequence
star: false
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300990_sequence/1

HR1-GAL1
type: ComponentDefinition
persistent identity: BBa K300986
display id: BBa_K300986
version: 1
was generated by: iGEM to SBOL conversion
description: Homologous region in S288C yeast genome (GAL1 ORF)
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: -
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300986_sequence/1

AvrII
type: ComponentDefinition
persistent identity: BBa B0048
display id: BBa_B0048
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_B0048_sequence/1

AvrII site
type: SequenceAnnotation
persistent identity: annotation1822744
display id: annotation1822744
version: 1
top level: AvrII
location: https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
type: Range
persistent identity: range1822744
display id: range1822744
version: 1
top level: AvrII
direction: direction/forward
start: 1
end: 6
orientation: inline

EcoRI
type: ComponentDefinition
persistent identity: BBa M31983
display id: BBa_M31983
version: 1
was generated by: iGEM to SBOL conversion
description: EcoRI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31983_sequence/1

MfeI
type: ComponentDefinition
persistent identity: BBa B0047
display id: BBa_B0047
version: 1
was generated by: iGEM to SBOL conversion
description: MfeI restriction enzyme site (EcoRI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: MfeI recognition site. Digestion with MfeI yields a compatible cohesive end to EcoRI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: MfeI site
sequence: https://synbiohub.org/public/igem/BBa_B0047_sequence/1

MfeI site
type: SequenceAnnotation
persistent identity: annotation1822740
display id: annotation1822740
version: 1
top level: MfeI
location: https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1
type: Range
persistent identity: range1822740
display id: range1822740
version: 1
top level: MfeI
direction: direction/forward
start: 1
end: 6
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

KanMX(lox)
type: ComponentDefinition
persistent identity: BBa K300989
display id: BBa_K300989
version: 1
was generated by: iGEM to SBOL conversion
description: [LoxP-KanMX-LoxP] excisable marker for yeast
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: pUG6 vector (GenBank: AF298793.1).
owned by: james
owned by: myers
mutable description: This cassette is to be used in yeast. It is composed by the kanMX gene from the E. coli transposon Tn903 that confers the resistance to the aminoglycoside antibiotic G418 to yeast (commonly used at 200 ug/ml working concentration). This gene is expressed by the TEF2 promoter/RBS and terminated by the TEF2 transcriptional terminator from Ashbya gossypii filamentous fungus. The cassette is flanked by two loxP sites and for this reason it can be excised with Cre/loxP mediated marker removal recombination procedure. ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to select positive integrants in the yeast genome and then it can be eliminated through Cre/loxP procedure. This enables the engineering of yeast genome in multiple steps, always using the same dominant selection marker.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The original sequence of loxP-KanMX-loxP from pUG6 vector has two NsiI restriction sites in kanR(Tn903) coding sequence and they have been removed in this part by changing codons. The TEF2 promoter has a XbaI and a PstI restriction sites, which have not been removed to avoid the change of the promoter strength. For this reason, this part is not fully compatible with RFC10 BioBrick Standard Assembly, but it can be assembled by using EcoRI-SpeI enzymes.
star: false
creator: Lorenzo Pasotti, Giacomo Zambianchi, Federica Sampietro, Paolo Magni
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: BBa_J61046 (loxP)
sequence annotation: TEF2 (A. gossypii)+RBS
sequence annotation: kanMX cassette
sequence annotation: kanR(Tn903)
sequence annotation: A->T
sequence annotation: T->C
sequence annotation: TEF2 terminator (A. gossypii)
sequence: https://synbiohub.org/public/igem/BBa_K300989_sequence/1

BBa_J61046 (loxP)
type: SequenceAnnotation
persistent identity: annotation2081360
display id: annotation2081360
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081360/range2081360/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K300989/annotation2081360/range2081360/1
type: Range
persistent identity: range2081360
display id: range2081360
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 1
end: 34
orientation: inline

TEF2 (A. gossypii)+RBS
type: SequenceAnnotation
persistent identity: annotation2081361
display id: annotation2081361
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081361/range2081361/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K300989/annotation2081361/range2081361/1
type: Range
persistent identity: range2081361
display id: range2081361
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 35
end: 432
orientation: inline

kanMX cassette
type: SequenceAnnotation
persistent identity: annotation2081367
display id: annotation2081367
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081367/range2081367/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K300989/annotation2081367/range2081367/1
type: Range
persistent identity: range2081367
display id: range2081367
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 35
end: 1507
orientation: inline

kanR(Tn903)
type: SequenceAnnotation
persistent identity: annotation2081362
display id: annotation2081362
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081362/range2081362/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K300989/annotation2081362/range2081362/1
type: Range
persistent identity: range2081362
display id: range2081362
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 433
end: 1242
orientation: inline

A->T
type: SequenceAnnotation
persistent identity: annotation2081363
display id: annotation2081363
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081363/range2081363/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K300989/annotation2081363/range2081363/1
type: Range
persistent identity: range2081363
display id: range2081363
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 708
end: 708
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation2081364
display id: annotation2081364
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081364/range2081364/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K300989/annotation2081364/range2081364/1
type: Range
persistent identity: range2081364
display id: range2081364
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 975
end: 975
orientation: inline

TEF2 terminator (A. gossypii)
type: SequenceAnnotation
persistent identity: annotation2081365
display id: annotation2081365
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081365/range2081365/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K300989/annotation2081365/range2081365/1
type: Range
persistent identity: range2081365
display id: range2081365
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 1243
end: 1507
orientation: inline

BBa_J61046 (loxP)
type: SequenceAnnotation
persistent identity: annotation2081366
display id: annotation2081366
version: 1
top level: KanMX(lox)
location: https://synbiohub.org/public/igem/BBa_K300989/annotation2081366/range2081366/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K300989/annotation2081366/range2081366/1
type: Range
persistent identity: range2081366
display id: range2081366
version: 1
top level: KanMX(lox)
direction: direction/forward
start: 1508
end: 1541
orientation: inline

HR2-GAL10
type: ComponentDefinition
persistent identity: BBa K300987
display id: BBa_K300987
version: 1
was generated by: iGEM to SBOL conversion
description: Homologous region in S288C yeast genome (GAL10 ORF)
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: -
star: false
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300987_sequence/1

SpeI
type: ComponentDefinition
persistent identity: BBa M31992
display id: BBa_M31992
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: SpeI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31992_sequence/1

NsiI
type: ComponentDefinition
persistent identity: BBa B0046
display id: BBa_B0046
version: 1
was generated by: iGEM to SBOL conversion
description: NsiI restriction enzyme site (PstI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NsiI recognition site. Digestion with NsiI generates a compatible cohesive end to PstI.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: NsiI site
sequence: https://synbiohub.org/public/igem/BBa_B0046_sequence/1

NsiI site
type: SequenceAnnotation
persistent identity: annotation1822730
display id: annotation1822730
version: 1
top level: NsiI
location: https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
type: Range
persistent identity: range1822730
display id: range1822730
version: 1
top level: NsiI
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_K300980_sequence/1
type: Sequence
persistent identity: BBa K300980 sequence
display id: BBa_K300980_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattgcctgcaggcctagggtgtgaaccaatgtatccagcaccacctgtaaccaaaacaattttcctagggaattctcacacaggactactagtataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttatgctagccctgagttcaattctagcgcaaaggaattaccaagaccattggccgctagccctgcaggatgcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300990_sequence/1
type: Sequence
persistent identity: BBa K300990 sequence
display id: BBa_K300990_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgcagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300986_sequence/1
type: Sequence
persistent identity: BBa K300986 sequence
display id: BBa_K300986_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgtgaaccaatgtatccagcaccacctgtaaccaaaacaatttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300989_sequence/1
type: Sequence
persistent identity: BBa K300989 sequence
display id: BBa_K300989_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcttggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0048_sequence/1
type: Sequence
persistent identity: BBa B0048 sequence
display id: BBa_B0048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31983_sequence/1
type: Sequence
persistent identity: BBa M31983 sequence
display id: BBa_M31983_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0047_sequence/1
type: Sequence
persistent identity: BBa B0047 sequence
display id: BBa_B0047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300987_sequence/1
type: Sequence
persistent identity: BBa K300987 sequence
display id: BBa_K300987_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgagttcaattctagcgcaaaggaattaccaagaccattggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31992_sequence/1
type: Sequence
persistent identity: BBa M31992 sequence
display id: BBa_M31992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0046_sequence/1
type: Sequence
persistent identity: BBa B0046 sequence
display id: BBa_B0046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z