##gff-version 3
##sequence-region BBa_K300982 1 2151
BBa_K300982	.	terminator	1986	2054	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_K300982	.	sequence_feature	1989	2028	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_K300982	.	stem_loop	1996	2027	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_K300982	.	sequence_feature	2055	2074	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_K300982	.	primer_binding_site	2055	2074	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_K300982	.	ribosome_entry_site	1969	1979	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K300982	.	engineered_region	1969	1979	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K300982	.	ribosome_entry_site	1969	1979	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K300982	.	ribosome_entry_site	1975	1978	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K300982	.	primer	1865	1884	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_K300982	.	primer_binding_site	1865	1884	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
BBa_K300982	.	sequence_feature	414	421	.	+	0	ID=BBa_G0001;Name=BBa_G0001
BBa_K300982	.	sequence_feature	1328	1334	.	+	0	ID=BBa_K300995;Name=BBa_K300995
BBa_K300982	.	sequence_feature	1963	1968	.	+	0	ID=BBa_M31983;Name=BBa_M31983
BBa_K300982	.	terminator	2075	2115	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_K300982	.	stem_loop	2088	2107	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
BBa_K300982	.	sequence_feature	1335	1370	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_K300982	.	terminator	1885	1962	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_K300982	.	sequence_feature	1912	1950	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_K300982	.	stem_loop	1920	1941	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_K300982	.	sequence_feature	1371	1376	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300982	.	sequence_feature	1371	1376	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300982	.	engineered_region	559	1327	.	+	0	ID=BBa_K300999;Name=BBa_K300999
BBa_K300982	.	CDS	559	1221	.	+	0	ID=annotation2081291;Name=cmR(rev);Parent=BBa_K300999
BBa_K300982	.	sequence_feature	1	7	.	+	0	ID=BBa_K300994;Name=BBa_K300994
BBa_K300982	.	terminator	422	550	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_K300982	.	engineered_region	471	550	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_K300982	.	engineered_region	422	462	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_K300982	.	terminator	1793	1864	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_K300982	.	stem_loop	1801	1835	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_K300982	.	sequence_feature	2116	2151	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_K300982	.	sequence_feature	1787	1792	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300982	.	sequence_feature	1787	1792	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300982	.	sequence_feature	1980	1985	.	+	0	ID=BBa_K300981;Name=BBa_K300981
BBa_K300982	.	sequence_feature	1377	1786	.	+	0	ID=BBa_K300991;Name=BBa_K300991
BBa_K300982	.	sequence_feature	8	413	.	+	0	ID=BBa_K300998;Name=BBa_K300998
BBa_K300982	.	sequence_feature	551	558	.	+	0	ID=BBa_G0001;Name=BBa_G0001
>BBa_K300982
cctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtact
aagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtt
tcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttt
tataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaac
gcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagcc
atgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagaga
gcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctc
tagtattattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccat
cacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaa
aacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagaca
aaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgt
ggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgta
acaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcagg
cgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatcca
gctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatat
atcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaat
acgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaacctctagggcgaagttc
ctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcgatatcatctcttttc
ttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgt
acacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcct
gctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtg
ttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcaca
ttaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaaaaacgggcaaggtgt
caccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattca
gcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattctcacaca
ggacctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
gctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatacttt
ctagagaataggaacttc