BBa_K300982
type: ComponentDefinition
persistent identity: BBa K300982
display id: BBa_K300982
version: 1
was generated by: iGEM to SBOL conversion
description: BBa_K300000 construction intermediate
created: 2010-10-08T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: <partinfo>BBa_K300983</partinfo> - Mr Gene synthesis service; <partinfo>BBa_K300008</partinfo> - UNIPV-Pavia iGEM 2010 Team.
owned by: james
owned by: myers
mutable description: This part was used to construct <partinfo>BBa_K300000</partinfo> BioBrick integrative base vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300000</partinfo> for details about the design of this part.
star: false
creator: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J72001
component: BBa_B0045
component: BBa_K300991
component: BBa_G00100
component: BBa_B0053
component: BBa_M31983
component: BBa_B0055
component: BBa_K300981
component: BBa_B0033
component: BBa_G00102
component: BBa_B0054
component: BBa_B0062
component: BBa_K300994
component: BBa_G0001
component: BBa_K300998
component: BBa_B0025
component: BBa_K300995
component: BBa_K300999
sequence annotation: BBa_K300994
sequence annotation: BBa_K300998
sequence annotation: BBa_G0001
sequence annotation: BBa_B0025
sequence annotation: BBa_K300999
sequence annotation: BBa_K300995
sequence annotation: BBa_J72001
sequence annotation: BBa_B0045
sequence annotation: BBa_K300991
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_M31983
sequence annotation: BBa_B0033
sequence annotation: BBa_K300981
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence: https://synbiohub.org/public/igem/BBa_K300982_sequence/1

BBa_J72001
type: Component
persistent identity: component2084547
display id: component2084547
version: 1
top level: BBa_K300982
definition: FRT
access: public

BBa_B0045
type: Component
persistent identity: component2084552
display id: component2084552
version: 1
top level: BBa_K300982
definition: NheI
access: public

BBa_K300991
type: Component
persistent identity: component2084550
display id: component2084550
version: 1
top level: BBa_K300982
definition: phi80 attP
access: public

BBa_G00100
type: Component
persistent identity: component2084556
display id: component2084556
version: 1
top level: BBa_K300982
definition: VF2
access: public

BBa_B0053
type: Component
persistent identity: component2084554
display id: component2084554
version: 1
top level: BBa_K300982
definition: BBa_B0053
access: public

BBa_M31983
type: Component
persistent identity: component2084560
display id: component2084560
version: 1
top level: BBa_K300982
definition: EcoRI
access: public

BBa_B0055
type: Component
persistent identity: component2084559
display id: component2084559
version: 1
top level: BBa_K300982
definition: BBa_B0055
access: public

BBa_K300981
type: Component
persistent identity: component2084564
display id: component2084564
version: 1
top level: BBa_K300982
definition: PstI
access: public

BBa_B0033
type: Component
persistent identity: component2084562
display id: component2084562
version: 1
top level: BBa_K300982
definition: BBa_B0033
access: public

BBa_G00102
type: Component
persistent identity: component2084569
display id: component2084569
version: 1
top level: BBa_K300982
definition: VR
access: public

BBa_B0054
type: Component
persistent identity: component2084567
display id: component2084567
version: 1
top level: BBa_K300982
definition: BBa_B0054
access: public

BBa_J72001
type: Component
persistent identity: component2084572
display id: component2084572
version: 1
top level: BBa_K300982
definition: FRT
access: public

BBa_B0062
type: Component
persistent identity: component2084571
display id: component2084571
version: 1
top level: BBa_K300982
definition: BBa_B0062
access: public

BBa_K300994
type: Component
persistent identity: component2084537
display id: component2084537
version: 1
top level: BBa_K300982
definition: scar
access: public

BBa_G0001
type: Component
persistent identity: component2084539
display id: component2084539
version: 1
top level: BBa_K300982
definition: scar
access: public

BBa_K300998
type: Component
persistent identity: component2084538
display id: component2084538
version: 1
top level: BBa_K300982
definition: R6K ori
access: public

BBa_G0001
type: Component
persistent identity: component2084543
display id: component2084543
version: 1
top level: BBa_K300982
definition: scar
access: public

BBa_B0025
type: Component
persistent identity: component2084542
display id: component2084542
version: 1
top level: BBa_K300982
definition: BBa_B0025
access: public

BBa_K300995
type: Component
persistent identity: component2084546
display id: component2084546
version: 1
top level: BBa_K300982
definition: scar
access: public

BBa_K300999
type: Component
persistent identity: component2084545
display id: component2084545
version: 1
top level: BBa_K300982
definition: CmR
access: public

BBa_B0045
type: Component
persistent identity: component2084549
display id: component2084549
version: 1
top level: BBa_K300982
definition: NheI
access: public

BBa_K300994
type: SequenceAnnotation
persistent identity: annotation2084537
display id: annotation2084537
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084537/range2084537/1
component: BBa_K300994

https://synbiohub.org/public/igem/BBa_K300982/annotation2084537/range2084537/1
type: Range
persistent identity: range2084537
display id: range2084537
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1
end: 7
orientation: inline

BBa_K300998
type: SequenceAnnotation
persistent identity: annotation2084538
display id: annotation2084538
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084538/range2084538/1
component: BBa_K300998

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type: Range
persistent identity: range2084538
display id: range2084538
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 8
end: 413
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2084539
display id: annotation2084539
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084539/range2084539/1
component: BBa_G0001

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type: Range
persistent identity: range2084539
display id: range2084539
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 414
end: 421
orientation: inline

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2084542
display id: annotation2084542
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084542/range2084542/1
component: BBa_B0025

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type: Range
persistent identity: range2084542
display id: range2084542
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 422
end: 550
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2084543
display id: annotation2084543
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084543/range2084543/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_K300982/annotation2084543/range2084543/1
type: Range
persistent identity: range2084543
display id: range2084543
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 551
end: 558
orientation: inline

BBa_K300999
type: SequenceAnnotation
persistent identity: annotation2084545
display id: annotation2084545
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084545/range2084545/1
component: BBa_K300999

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type: Range
persistent identity: range2084545
display id: range2084545
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 559
end: 1327
orientation: inline

BBa_K300995
type: SequenceAnnotation
persistent identity: annotation2084546
display id: annotation2084546
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084546/range2084546/1
component: BBa_K300995

https://synbiohub.org/public/igem/BBa_K300982/annotation2084546/range2084546/1
type: Range
persistent identity: range2084546
display id: range2084546
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1328
end: 1334
orientation: inline

BBa_J72001
type: SequenceAnnotation
persistent identity: annotation2084547
display id: annotation2084547
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084547/range2084547/1
component: BBa_J72001

https://synbiohub.org/public/igem/BBa_K300982/annotation2084547/range2084547/1
type: Range
persistent identity: range2084547
display id: range2084547
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1335
end: 1370
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2084549
display id: annotation2084549
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084549/range2084549/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300982/annotation2084549/range2084549/1
type: Range
persistent identity: range2084549
display id: range2084549
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1371
end: 1376
orientation: inline

BBa_K300991
type: SequenceAnnotation
persistent identity: annotation2084550
display id: annotation2084550
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084550/range2084550/1
component: BBa_K300991

https://synbiohub.org/public/igem/BBa_K300982/annotation2084550/range2084550/1
type: Range
persistent identity: range2084550
display id: range2084550
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1377
end: 1786
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2084552
display id: annotation2084552
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084552/range2084552/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300982/annotation2084552/range2084552/1
type: Range
persistent identity: range2084552
display id: range2084552
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1787
end: 1792
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2084554
display id: annotation2084554
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084554/range2084554/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K300982/annotation2084554/range2084554/1
type: Range
persistent identity: range2084554
display id: range2084554
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1793
end: 1864
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2084556
display id: annotation2084556
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084556/range2084556/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K300982/annotation2084556/range2084556/1
type: Range
persistent identity: range2084556
display id: range2084556
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1865
end: 1884
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2084559
display id: annotation2084559
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084559/range2084559/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K300982/annotation2084559/range2084559/1
type: Range
persistent identity: range2084559
display id: range2084559
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1885
end: 1962
orientation: inline

BBa_M31983
type: SequenceAnnotation
persistent identity: annotation2084560
display id: annotation2084560
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084560/range2084560/1
component: BBa_M31983

https://synbiohub.org/public/igem/BBa_K300982/annotation2084560/range2084560/1
type: Range
persistent identity: range2084560
display id: range2084560
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1963
end: 1968
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2084562
display id: annotation2084562
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084562/range2084562/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K300982/annotation2084562/range2084562/1
type: Range
persistent identity: range2084562
display id: range2084562
version: 1
top level: BBa_K300982
direction: direction/forward
start: 1969
end: 1979
orientation: inline

BBa_K300981
type: SequenceAnnotation
persistent identity: annotation2084564
display id: annotation2084564
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084564/range2084564/1
component: BBa_K300981

https://synbiohub.org/public/igem/BBa_K300982/annotation2084564/range2084564/1
type: Range
persistent identity: range2084564
display id: range2084564
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1980
end: 1985
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2084567
display id: annotation2084567
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084567/range2084567/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K300982/annotation2084567/range2084567/1
type: Range
persistent identity: range2084567
display id: range2084567
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 1986
end: 2054
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2084569
display id: annotation2084569
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084569/range2084569/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K300982/annotation2084569/range2084569/1
type: Range
persistent identity: range2084569
display id: range2084569
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 2055
end: 2074
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2084571
display id: annotation2084571
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084571/range2084571/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K300982/annotation2084571/range2084571/1
type: Range
persistent identity: range2084571
display id: range2084571
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 2075
end: 2115
orientation: inline

BBa_J72001
type: SequenceAnnotation
persistent identity: annotation2084572
display id: annotation2084572
version: 1
top level: BBa_K300982
location: https://synbiohub.org/public/igem/BBa_K300982/annotation2084572/range2084572/1
component: BBa_J72001

https://synbiohub.org/public/igem/BBa_K300982/annotation2084572/range2084572/1
type: Range
persistent identity: range2084572
display id: range2084572
version: 1
top level: BBa_K300982
direction: direction/reverse
start: 2116
end: 2151
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

FRT
type: ComponentDefinition
persistent identity: BBa J72001
display id: BBa_J72001
version: 1
was generated by: iGEM to SBOL conversion
description: {FRT} recombination site for flp recombinase in BBb
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:26Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/None
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J72001_sequence/1

R6K ori
type: ComponentDefinition
persistent identity: BBa K300998
display id: BBa_K300998
version: 1
was generated by: iGEM to SBOL conversion
description: [R6K] Origin of replication (rev)
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: See <partinfo>BBa_J61001</partinfo> for detailed information.
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin).
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: <partinfo>BBa_J61001</partinfo>
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300998_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa K300994
display id: BBa_K300994
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_K300996</partinfo>.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300996</partinfo>
star: false
creator: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300994_sequence/1

phi80 attP
type: ComponentDefinition
persistent identity: BBa K300991
display id: BBa_K300991
version: 1
was generated by: iGEM to SBOL conversion
description: phi80 phage attP integration site
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end.
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: See <partinfo>BBa_J72004</partinfo> for more information.
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300991_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa G0001
display id: BBa_G0001
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI/SpeI mixed site, reverse
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site, reverse
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_G0001_sequence/1

PstI
type: ComponentDefinition
persistent identity: BBa K300981
display id: BBa_K300981
version: 1
was generated by: iGEM to SBOL conversion
description: PstI restriction enzyme site
created: 2010-10-08T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300981_sequence/1

EcoRI
type: ComponentDefinition
persistent identity: BBa M31983
display id: BBa_M31983
version: 1
was generated by: iGEM to SBOL conversion
description: EcoRI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31983_sequence/1

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0015), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0012
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 1
end: 41
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 50
end: 129
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa K300999
display id: BBa_K300999
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette (rev)
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: <partinfo>BBa_P1004</partinfo>
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator).
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: see <partinfo>BBa_P1004</partinfo> for detailed information.
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR(rev)
sequence: https://synbiohub.org/public/igem/BBa_K300999_sequence/1

cmR(rev)
type: SequenceAnnotation
persistent identity: annotation2081291
display id: annotation2081291
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_K300999/annotation2081291/range2081291/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K300999/annotation2081291/range2081291/1
type: Range
persistent identity: range2081291
display id: range2081291
version: 1
top level: CmR
direction: direction/reverse
start: 1
end: 663
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa K300995
display id: BBa_K300995
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_K300997</partinfo>
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300997</partinfo>
star: false
creator: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300995_sequence/1

https://synbiohub.org/public/igem/BBa_K300982_sequence/1
type: Sequence
persistent identity: BBa K300982 sequence
display id: BBa_K300982_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattctcacacaggacctgcagattagcagaaagtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0025_sequence/1
type: Sequence
persistent identity: BBa B0025 sequence
display id: BBa_B0025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J72001_sequence/1
type: Sequence
persistent identity: BBa J72001 sequence
display id: BBa_J72001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgaagttcctatactttctagagaataggaacttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300999_sequence/1
type: Sequence
persistent identity: BBa K300999 sequence
display id: BBa_K300999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300998_sequence/1
type: Sequence
persistent identity: BBa K300998 sequence
display id: BBa_K300998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300995_sequence/1
type: Sequence
persistent identity: BBa K300995 sequence
display id: BBa_K300995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300994_sequence/1
type: Sequence
persistent identity: BBa K300994 sequence
display id: BBa_K300994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300991_sequence/1
type: Sequence
persistent identity: BBa K300991 sequence
display id: BBa_K300991_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0001_sequence/1
type: Sequence
persistent identity: BBa G0001 sequence
display id: BBa_G0001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300981_sequence/1
type: Sequence
persistent identity: BBa K300981 sequence
display id: BBa_K300981_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31983_sequence/1
type: Sequence
persistent identity: BBa M31983 sequence
display id: BBa_M31983_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z