<partinfo>BBa_K300983</partinfo> - Mr Gene synthesis service; <partinfo>BBa_K300008</partinfo> - UNIPV-Pavia iGEM 2010 Team.
This part was used to construct <partinfo>BBa_K300000</partinfo> BioBrick integrative base vector.
See <partinfo>BBa_K300000</partinfo> for details about the design of this part.
Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
Forward primer for sequencing/amplifying BioBrick parts (VF2)
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
Reverse BioBrick primer annealing site (VR binding site)
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
RBS.4 (weaker) -- derivative of BBa_0030
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
{FRT} recombination site for flp recombinase in BBb
See <partinfo>BBa_J61001</partinfo> for detailed information.
Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin).
Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev
Reverse complement of <partinfo>BBa_K300996</partinfo>.
This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end.
See <partinfo>BBa_J72004</partinfo> for more information.
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
chloramphenicol resistance cassette (rev)
Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator).
see <partinfo>BBa_P1004</partinfo> for detailed information.
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev
Reverse complement of <partinfo>BBa_K300997</partinfo>
Conversion of the iGEM parts registry to SBOL2.1