##gff-version 3
##sequence-region BBa_K300983 1 829
BBa_K300983	.	sequence_feature	788	823	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_K300983	.	ribosome_entry_site	641	651	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K300983	.	engineered_region	641	651	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K300983	.	ribosome_entry_site	641	651	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K300983	.	ribosome_entry_site	647	650	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K300983	.	terminator	557	634	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_K300983	.	stem_loop	592	613	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_K300983	.	sequence_feature	584	622	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_K300983	.	primer	537	556	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_K300983	.	primer_binding_site	537	556	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
BBa_K300983	.	sequence_feature	459	464	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300983	.	sequence_feature	459	464	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300983	.	sequence_feature	727	746	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_K300983	.	primer_binding_site	727	746	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_K300983	.	sequence_feature	43	48	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_K300983	.	sequence_feature	43	48	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_K300983	.	sequence_feature	824	829	.	+	0	ID=BBa_B0048;Name=BBa_B0048
BBa_K300983	.	sequence_feature	824	829	.	+	0	ID=annotation1822744;Name=AvrII site;Parent=BBa_B0048
BBa_K300983	.	sequence_feature	635	640	.	+	0	ID=BBa_M31983;Name=BBa_M31983
BBa_K300983	.	terminator	747	787	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_K300983	.	stem_loop	760	779	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
BBa_K300983	.	terminator	658	726	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_K300983	.	sequence_feature	661	700	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_K300983	.	stem_loop	668	699	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_K300983	.	sequence_feature	7	42	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_K300983	.	terminator	465	536	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_K300983	.	stem_loop	473	507	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_K300983	.	sequence_feature	1	6	.	+	0	ID=BBa_B0048;Name=BBa_B0048
BBa_K300983	.	sequence_feature	1	6	.	+	0	ID=annotation1822744;Name=AvrII site;Parent=BBa_B0048
BBa_K300983	.	sequence_feature	652	657	.	+	0	ID=BBa_K300981;Name=BBa_K300981
BBa_K300983	.	sequence_feature	49	458	.	+	0	ID=BBa_K300991;Name=BBa_K300991
>BBa_K300983
cctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcg
atatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttg
ggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggttta
catccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatga
caatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaat
aaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaa
aaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaa
gaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgt
aagaattctcacacaggacctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttccctt
ggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcg
aagttcctatactttctagagaataggaacttccctagg