BBa_K300983
type: ComponentDefinition
persistent identity: BBa K300983
display id: BBa_K300983
version: 1
was generated by: iGEM to SBOL conversion
description: BBa_K300000 construction intermediate (provided by Mr Gene)
created: 2010-10-08T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Mr Gene synthesis service (www.mrgene.com).
owned by: james
owned by: myers
mutable description: This part has been provided by Mr Gene synthesis service and it has been used to construct <partinfo>BBa_K300982</partinfo> plasmid. On the other hand, <partinfo>BBa_K300982</partinfo> was used to construct <partinfo>BBa_K300000</partinfo> BioBrick integrative base vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300000</partinfo> for details about the design of this part.
star: false
creator: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0045
component: BBa_J72001
component: BBa_K300991
component: BBa_G00100
component: BBa_B0053
component: BBa_M31983
component: BBa_B0055
component: BBa_K300981
component: BBa_B0033
component: BBa_G00102
component: BBa_B0054
component: BBa_B0062
component: BBa_B0048
sequence annotation: BBa_B0048
sequence annotation: BBa_J72001
sequence annotation: BBa_B0045
sequence annotation: BBa_K300991
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_M31983
sequence annotation: BBa_B0033
sequence annotation: BBa_K300981
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence: https://synbiohub.org/public/igem/BBa_K300983_sequence/1

BBa_B0045
type: Component
persistent identity: component2084439
display id: component2084439
version: 1
top level: BBa_K300983
definition: NheI
access: public

BBa_J72001
type: Component
persistent identity: component2084437
display id: component2084437
version: 1
top level: BBa_K300983
definition: FRT
access: public

BBa_B0045
type: Component
persistent identity: component2084442
display id: component2084442
version: 1
top level: BBa_K300983
definition: NheI
access: public

BBa_K300991
type: Component
persistent identity: component2084440
display id: component2084440
version: 1
top level: BBa_K300983
definition: phi80 attP
access: public

BBa_G00100
type: Component
persistent identity: component2084446
display id: component2084446
version: 1
top level: BBa_K300983
definition: VF2
access: public

BBa_B0053
type: Component
persistent identity: component2084444
display id: component2084444
version: 1
top level: BBa_K300983
definition: BBa_B0053
access: public

BBa_M31983
type: Component
persistent identity: component2084450
display id: component2084450
version: 1
top level: BBa_K300983
definition: EcoRI
access: public

BBa_B0055
type: Component
persistent identity: component2084449
display id: component2084449
version: 1
top level: BBa_K300983
definition: BBa_B0055
access: public

BBa_K300981
type: Component
persistent identity: component2084454
display id: component2084454
version: 1
top level: BBa_K300983
definition: PstI
access: public

BBa_B0033
type: Component
persistent identity: component2084452
display id: component2084452
version: 1
top level: BBa_K300983
definition: BBa_B0033
access: public

BBa_G00102
type: Component
persistent identity: component2084459
display id: component2084459
version: 1
top level: BBa_K300983
definition: VR
access: public

BBa_B0054
type: Component
persistent identity: component2084457
display id: component2084457
version: 1
top level: BBa_K300983
definition: BBa_B0054
access: public

BBa_J72001
type: Component
persistent identity: component2084462
display id: component2084462
version: 1
top level: BBa_K300983
definition: FRT
access: public

BBa_B0062
type: Component
persistent identity: component2084461
display id: component2084461
version: 1
top level: BBa_K300983
definition: BBa_B0062
access: public

BBa_B0048
type: Component
persistent identity: component2084464
display id: component2084464
version: 1
top level: BBa_K300983
definition: AvrII
access: public

BBa_B0048
type: Component
persistent identity: component2084436
display id: component2084436
version: 1
top level: BBa_K300983
definition: AvrII
access: public

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2084436
display id: annotation2084436
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084436/range2084436/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300983/annotation2084436/range2084436/1
type: Range
persistent identity: range2084436
display id: range2084436
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 1
end: 6
orientation: inline

BBa_J72001
type: SequenceAnnotation
persistent identity: annotation2084437
display id: annotation2084437
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084437/range2084437/1
component: BBa_J72001

https://synbiohub.org/public/igem/BBa_K300983/annotation2084437/range2084437/1
type: Range
persistent identity: range2084437
display id: range2084437
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 7
end: 42
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2084439
display id: annotation2084439
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084439/range2084439/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300983/annotation2084439/range2084439/1
type: Range
persistent identity: range2084439
display id: range2084439
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 43
end: 48
orientation: inline

BBa_K300991
type: SequenceAnnotation
persistent identity: annotation2084440
display id: annotation2084440
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084440/range2084440/1
component: BBa_K300991

https://synbiohub.org/public/igem/BBa_K300983/annotation2084440/range2084440/1
type: Range
persistent identity: range2084440
display id: range2084440
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 49
end: 458
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2084442
display id: annotation2084442
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084442/range2084442/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300983/annotation2084442/range2084442/1
type: Range
persistent identity: range2084442
display id: range2084442
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 459
end: 464
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2084444
display id: annotation2084444
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084444/range2084444/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K300983/annotation2084444/range2084444/1
type: Range
persistent identity: range2084444
display id: range2084444
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 465
end: 536
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2084446
display id: annotation2084446
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084446/range2084446/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K300983/annotation2084446/range2084446/1
type: Range
persistent identity: range2084446
display id: range2084446
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 537
end: 556
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2084449
display id: annotation2084449
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084449/range2084449/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K300983/annotation2084449/range2084449/1
type: Range
persistent identity: range2084449
display id: range2084449
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 557
end: 634
orientation: inline

BBa_M31983
type: SequenceAnnotation
persistent identity: annotation2084450
display id: annotation2084450
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084450/range2084450/1
component: BBa_M31983

https://synbiohub.org/public/igem/BBa_K300983/annotation2084450/range2084450/1
type: Range
persistent identity: range2084450
display id: range2084450
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 635
end: 640
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2084452
display id: annotation2084452
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084452/range2084452/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K300983/annotation2084452/range2084452/1
type: Range
persistent identity: range2084452
display id: range2084452
version: 1
top level: BBa_K300983
direction: direction/forward
start: 641
end: 651
orientation: inline

BBa_K300981
type: SequenceAnnotation
persistent identity: annotation2084454
display id: annotation2084454
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084454/range2084454/1
component: BBa_K300981

https://synbiohub.org/public/igem/BBa_K300983/annotation2084454/range2084454/1
type: Range
persistent identity: range2084454
display id: range2084454
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 652
end: 657
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2084457
display id: annotation2084457
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084457/range2084457/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K300983/annotation2084457/range2084457/1
type: Range
persistent identity: range2084457
display id: range2084457
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 658
end: 726
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2084459
display id: annotation2084459
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084459/range2084459/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K300983/annotation2084459/range2084459/1
type: Range
persistent identity: range2084459
display id: range2084459
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 727
end: 746
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2084461
display id: annotation2084461
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084461/range2084461/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K300983/annotation2084461/range2084461/1
type: Range
persistent identity: range2084461
display id: range2084461
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 747
end: 787
orientation: inline

BBa_J72001
type: SequenceAnnotation
persistent identity: annotation2084462
display id: annotation2084462
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084462/range2084462/1
component: BBa_J72001

https://synbiohub.org/public/igem/BBa_K300983/annotation2084462/range2084462/1
type: Range
persistent identity: range2084462
display id: range2084462
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 788
end: 823
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2084464
display id: annotation2084464
version: 1
top level: BBa_K300983
location: https://synbiohub.org/public/igem/BBa_K300983/annotation2084464/range2084464/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300983/annotation2084464/range2084464/1
type: Range
persistent identity: range2084464
display id: range2084464
version: 1
top level: BBa_K300983
direction: direction/reverse
start: 824
end: 829
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

FRT
type: ComponentDefinition
persistent identity: BBa J72001
display id: BBa_J72001
version: 1
was generated by: iGEM to SBOL conversion
description: {FRT} recombination site for flp recombinase in BBb
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:26Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/None
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J72001_sequence/1

AvrII
type: ComponentDefinition
persistent identity: BBa B0048
display id: BBa_B0048
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_B0048_sequence/1

AvrII site
type: SequenceAnnotation
persistent identity: annotation1822744
display id: annotation1822744
version: 1
top level: AvrII
location: https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
type: Range
persistent identity: range1822744
display id: range1822744
version: 1
top level: AvrII
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

phi80 attP
type: ComponentDefinition
persistent identity: BBa K300991
display id: BBa_K300991
version: 1
was generated by: iGEM to SBOL conversion
description: phi80 phage attP integration site
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This part is equal to <partinfo>BBa_J72004</partinfo>, but it lacks the NheI restriction site (GCTAGC) at the 3' end.
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: See <partinfo>BBa_J72004</partinfo> for more information.
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300991_sequence/1

PstI
type: ComponentDefinition
persistent identity: BBa K300981
display id: BBa_K300981
version: 1
was generated by: iGEM to SBOL conversion
description: PstI restriction enzyme site
created: 2010-10-08T11:00:00Z
modified: 2015-05-08T01:11:51Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300981_sequence/1

EcoRI
type: ComponentDefinition
persistent identity: BBa M31983
display id: BBa_M31983
version: 1
was generated by: iGEM to SBOL conversion
description: EcoRI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31983_sequence/1

https://synbiohub.org/public/igem/BBa_K300983_sequence/1
type: Sequence
persistent identity: BBa K300983 sequence
display id: BBa_K300983_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagggcgaagttcctatactttctagagaataggaacttcgctagccatgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatcagctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattctcacacaggacctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatactttctagagaataggaacttccctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J72001_sequence/1
type: Sequence
persistent identity: BBa J72001 sequence
display id: BBa_J72001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgaagttcctatactttctagagaataggaacttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0048_sequence/1
type: Sequence
persistent identity: BBa B0048 sequence
display id: BBa_B0048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300991_sequence/1
type: Sequence
persistent identity: BBa K300991 sequence
display id: BBa_K300991_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catgggcccgtgcgaatcagaaataatctcgatatcatctcttttcttgccgagcgccttcctgagctttgtgtcggtgatcattgaatgggtacacatttttgtttttgggtacacaaaagtgtacacaaagttgcccactcaaagctacacgcaatgtaacactagttcgcagagtgttatggtttacatccttgaaagcctgctggataagggtttagcgtaacagaacgtttttacgcggaattgttcgtaatatgccaaatgacaatttaagaaagtgttctaatttattagaaatttgcacttaaatcaaaaagttacggacaattcaaccaccaatcaataaattaaagggcacattaaagtacacaatatttgtgcccttctctgtttctttcccgttatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300981_sequence/1
type: Sequence
persistent identity: BBa K300981 sequence
display id: BBa_K300981_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31983_sequence/1
type: Sequence
persistent identity: BBa M31983 sequence
display id: BBa_M31983_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z