BBa_K300984 1 BBa_K300984 E. coli strain BW25141 (pir+) 2010-10-08T11:00:00Z 2015-05-08T01:11:52Z Coli Genetic Stock Center: strain number CGSC#7635. This E. coli strain can be used to propagate plasmids with R6K conditional replication origin (<partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K300998</partinfo>), such as <partinfo>BBa_K300000</partinfo> integrative base vector after the excision of its insert (<partinfo>BBa_I52002</partinfo>). BW23474 propagates R6K origin at a copy number comparable to medium copy plasmids (~15 plasmids per cell) [1]. '''Genotype:''' F-, &#916;(araD-araB)567, &#916;lacZ4787(::rrnB-3), &#916;(phoB-phoR)580, &#955;-, galU95, &#916;uidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, &#916;(rhaD-rhaB)568, hsdR514 false false _420_ 0 2621 9 No part sequence true This is not the only strain able to propagate R6K at medium copy, but it has been mentioned in several papers and it is easily retrievable from the CGSC. false Manuel Lupotto, Lorenzo Pasotti, Paolo Magni igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z