BBa_K300985 1 BBa_K300985 E. coli strain BW23474 (pir-116) 2010-10-08T11:00:00Z 2015-05-08T01:11:52Z Coli Genetic Stock Center: strain number CGSC#7838. This E. coli strain can be used to propagate plasmids with R6K conditional replication origin (<partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K300998</partinfo>), such as <partinfo>BBa_K300000</partinfo> integrative base vector after the excision of its insert (<partinfo>BBa_I52002</partinfo>). BW23474 propagates R6K origin at a copy number comparable to high copy plasmids (~250 plasmids per cell) [1]. '''Genotype:''' F-, &#916;(argF-lac)169, &#916;uidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510 false false _420_ 0 2621 9 No part sequence true This is not the only strain able to propagate R6K at high copy, but it has been mentioned in several papers and it is easily retrievable from the CGSC. false Manuel Lupotto, Lorenzo Pasotti, Paolo Magni igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z