LOCUS       BBa_K300988             2036 bp    DNA     linear       24-Nov-2024
DEFINITION  pSB1A2 without Prefix and Suffix
ACCESSION   BBa_K300988
VERSION     BBa_K300988.1  
FEATURES             Location/Qualifiers
     primer_bind     58..77
                     /label=VR
     CDS             162..776
                     /label=rep (pMB1) (rev)
     misc_feature    177..177
                     /label=ORI
     CDS             936..1796
                     /label=APr (rev)
     misc_feature    1831..1831
                     /label=pBLA (rev)
     primer_bind     1919..1938
                     /label=VF2
     stem_loop       2008..2027
                     /label=terminator
ORIGIN
        1 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
       61 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctg
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