LOCUS       BBa_K300989             1541 bp    DNA     linear       01-Jun-2024
DEFINITION  [LoxP-KanMX-LoxP] excisable marker for yeast
ACCESSION   BBa_K300989
VERSION     BBa_K300989.1  
FEATURES             Location/Qualifiers
     misc_feature    1..34
                     /label=BBa_J61046 (loxP)
     promoter        35..432
                     /label=TEF2 (A. gossypii)+RBS
     CDS             433..1242
                     /label=kanR(Tn903)
     misc_feature    708..708
                     /label=A->T
     misc_feature    975..975
                     /label=T->C
     misc_feature    1243..1507
                     /label=TEF2 terminator (A. gossypii)
     misc_feature    1508..1541
                     /label=BBa_J61046 (loxP)
     misc_feature    35..1507
                     /label=kanMX cassette
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tagagatctg tttagcttgc
       61 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
      121 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
      181 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
      241 ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
      301 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
      361 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
      421 acataaacaa ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac
      481 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      541 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      601 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      661 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcttg gttactcacc
      721 actgcgatcc ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
      781 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      841 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      901 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      961 tggaaagaaa tgcacaagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1021 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1081 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1141 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1201 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa
     1261 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     1321 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     1381 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     1441 tactgctgtc gattcgatac taacgccgcc atccagtgtc gaaaacgagc tctcgagaac
     1501 ccttaatata acttcgtata atgtatgcta tacgaagtta t
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