LOCUS       BBa_K301001              942 bp    DNA     linear       31-Dec-2025
DEFINITION  pTAC with RFP reporter
ACCESSION   BBa_K301001
VERSION     BBa_K301001.1  
FEATURES             Location/Qualifiers
     promoter        1..73
                     /label=BBa_K301000
     misc_feature    86..89
                     /label=conserved
     RBS             82..93
                     /label=BBa_B0034
     CDS             100..774
                     /label=mrfp1
     misc_feature    781..805
                     /label=Help:Barcodes
     CDS             100..780
                     /label=BBa_E1010
     stem_loop       825..868
                     /label=stem_loop
     misc_feature    814..893
                     /label=BBa_B0010
     terminator      814..893
                     /label=BBa_B0010
     stem_loop       909..928
                     /label=T7 TE
     misc_feature    935..935
                     /label=stop
     polyA_site      929..942
                     /label=polya
     misc_feature    902..942
                     /label=BBa_B0012
     terminator      902..942
                     /label=BBa_B0012
     misc_feature    82..942
                     /label=BBa_I13507
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttcacacagg aaatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt
      121 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      181 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      241 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      301 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      361 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      421 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      481 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      541 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      601 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
      661 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
      721 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
      781 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc
      841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//