##gff-version 3
##sequence-region BBa_K301006 1 3082
BBa_K301006	.	CDS	146	1145	.	+	0	ID=BBa_K301005;Name=BBa_K301005
BBa_K301006	.	promoter	1154	1176	.	+	0	ID=BBa_K301003;Name=BBa_K301003
BBa_K301006	.	promoter	1154	1176	.	+	0	ID=annotation2099978;Name=promoter;Parent=BBa_K301003
BBa_K301006	.	CDS	1183	2182	.	+	0	ID=BBa_K301005;Name=BBa_K301005
BBa_K301006	.	promoter	2191	2213	.	+	0	ID=BBa_K301003;Name=BBa_K301003
BBa_K301006	.	promoter	2191	2213	.	+	0	ID=annotation2099978;Name=promoter;Parent=BBa_K301003
BBa_K301006	.	engineered_region	2222	3082	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K301006	.	ribosome_entry_site	2222	2233	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K301006	.	conserved_region	2226	2229	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K301006	.	terminator	2954	3033	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K301006	.	engineered_region	2954	3033	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K301006	.	stem_loop	2965	3008	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K301006	.	CDS	2240	2920	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K301006	.	CDS	2240	2914	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K301006	.	engineered_tag	2921	2945	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K301006	.	terminator	3042	3082	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K301006	.	engineered_region	3042	3082	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K301006	.	polyA_site	3069	3082	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K301006	.	stop_codon	3075	3075	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K301006	.	stem_loop	3049	3068	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K301006	.	promoter	1	139	.	+	0	ID=BBa_K239005;Name=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	34	45	.	+	0	ID=annotation2006716;Name=NarL;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	47	53	.	+	0	ID=annotation2006719;Name=NarL;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	88	106	.	+	0	ID=annotation2006714;Name=Fis;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	70	76	.	+	0	ID=annotation2006715;Name=NarL;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	4	16	.	+	0	ID=annotation2006710;Name=IHF;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	54	67	.	+	0	ID=annotation2006717;Name=Fnr;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	57	63	.	+	0	ID=annotation2006718;Name=NarL;Parent=BBa_K239005
BBa_K301006	.	promoter	126	133	.	+	0	ID=annotation2006711;Name=-10;Parent=BBa_K239005
BBa_K301006	.	non_covalent_binding_site	92	105	.	+	0	ID=annotation2006713;Name=Fnr;Parent=BBa_K239005
BBa_K301006	.	promoter	98	106	.	+	0	ID=annotation2006712;Name=-35;Parent=BBa_K239005
>BBa_K301006
gcaccgtgaaaaatctcataatttttatgaagtcactgtactcactatgggtaatgataaatatcaatgatagataaag
ttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagactactagatgaacacgatta
acatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcg
tttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgt
caacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcac
gcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaa
gccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggct
gtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttca
agaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctga
catgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgc
tgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctg
agactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgtt
ccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctg
gcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgatta
acattgcgcaaaacaccgcatggaaaatcaacaagaaagtactagagtaatacgactcactatagggagatactagatg
aacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccatt
acggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagat
gtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaag
atgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgc
aagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaac
cgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagct
aagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttg
tcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgt
aggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggt
caagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatct
ctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcg
tcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtac
aaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtactagagtaatacgactcactatagggag
atactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgta
tggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgc
taaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaa
gcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgtta
tgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaa
actgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgt
atgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctg
aagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcac
ctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgc
tgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a