LOCUS       BBa_K301007             3032 bp    DNA     linear       23-Dec-2024
DEFINITION  Low Oxygen Level Self-induction Genetic Circuit. mNarK promoter
ACCESSION   BBa_K301007
VERSION     BBa_K301007.1  
FEATURES             Location/Qualifiers
     promoter        76..83
                     /label=-10
     promoter        48..56
                     /label=-35
     misc_feature    42..55
                     /label=Fnr
     misc_feature    38..56
                     /label=Fis
     misc_feature    4..17
                     /label=Fnr
     misc_feature    20..26
                     /label=NarL
     misc_feature    7..13
                     /label=NarL
     promoter        1..89
                     /label=BBa_K239006
     CDS             96..1095
                     /label=BBa_K301005
     promoter        1104..1126
                     /label=promoter
     promoter        1104..1126
                     /label=BBa_K301003
     CDS             1133..2132
                     /label=BBa_K301005
     promoter        2141..2163
                     /label=promoter
     promoter        2141..2163
                     /label=BBa_K301003
     misc_feature    2176..2179
                     /label=conserved
     RBS             2172..2183
                     /label=BBa_B0034
     CDS             2190..2864
                     /label=mrfp1
     misc_feature    2871..2895
                     /label=Help:Barcodes
     CDS             2190..2870
                     /label=BBa_E1010
     stem_loop       2915..2958
                     /label=stem_loop
     misc_feature    2904..2983
                     /label=BBa_B0010
     terminator      2904..2983
                     /label=BBa_B0010
     stem_loop       2999..3018
                     /label=T7 TE
     misc_feature    3025..3025
                     /label=stop
     polyA_site      3019..3032
                     /label=polya
     misc_feature    2992..3032
                     /label=BBa_B0012
     terminator      2992..3032
                     /label=BBa_B0012
     misc_feature    2172..3032
                     /label=BBa_I13507
     misc_feature    96..3032
                     /label=BBa_K301010
ORIGIN
        1 gtattgataa atatcaatga tagataaagt tatcttatcg tttgatttac atcaaattgc
       61 ctttagctac agacactaag gtggcagact actagatgaa cacgattaac atcgctaaga
      121 acgacttctc tgacatcgaa ctggctgcta tcccgttcaa cactctggct gaccattacg
      181 gtgagcgttt agctcgcgaa cagttggccc ttgagcatga gtcttacgag atgggtgaag
      241 cacgcttccg caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg
      301 ccgccaagcc tctcatcact accctactcc ctaagatgat tgcacgcatc aacgactggt
      361 ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc cttccagttc ctgcaagaaa
      421 tcaagccgga agccgtagcg tacatcacca ttaagaccac tctggcttgc ctaaccagtg
      481 ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg
      541 ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt caagaaaaac gttgaggaac
      601 aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg
      661 acatgctctc taagggtcta ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact
      721 ctattcatgt aggagtacgc tgcatcgaga tgctcattga gtcaaccgga atggttagct
      781 tacaccgcca aaatgctggc gtagtaggtc aagactctga gactatcgaa ctcgcacctg
      841 aatacgctga ggctatcgca acccgtgcag gtgcgctggc tggcatctct ccgatgttcc
      901 aaccttgcgt agttcctcct aagccgtgga ctggcattac tggtggtggc tattgggcta
      961 acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg
     1021 aagacgttta catgcctgag gtgtacaaag cgattaacat tgcgcaaaac accgcatgga
     1081 aaatcaacaa gaaagtacta gagtaatacg actcactata gggagatact agatgaacac
     1141 gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac
     1201 tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc
     1261 ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga
     1321 ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc
     1381 acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt
     1441 ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct
     1501 ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg
     1561 ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa
     1621 gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat
     1681 gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc
     1741 gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc
     1801 aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac
     1861 tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg
     1921 catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg
     1981 tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa
     2041 agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc
     2101 gcaaaacacc gcatggaaaa tcaacaagaa agtactagag taatacgact cactataggg
     2161 agatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     2221 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     2281 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     2341 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     2401 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     2461 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     2521 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     2581 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     2641 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     2701 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     2761 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     2821 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
     2881 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2941 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     3001 ctcaccttcg ggtgggcctt tctgcgttta ta
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